Structure determination

Structure determination is a procedure by which the three-dimensional atomic coordinates of a molecule or biomolecule are solved using an analytical technique. Many techniques are used in structure determination, most commonly X-ray crystallography, NMR spectroscopy, electron microscopy and molecular modelling.

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Latest Research and Reviews

  • Research | | open

    The main components of tight junctions (TJ) are claudins that polymerize and form meshwork architectures called TJ strands. Here the authors present the 3.6 Å crystal structure of murine claudin-3 and show that residue P134 causes a bending of the third transmembrane helix which affects the morphology and adhesiveness of the TJ strands.

    • Shun Nakamura
    • , Katsumasa Irie
    • , Hiroo Tanaka
    • , Kouki Nishikawa
    • , Hiroshi Suzuki
    • , Yasunori Saitoh
    • , Atsushi Tamura
    • , Sachiko Tsukita
    •  & Yoshinori Fujiyoshi
  • Research | | open

    Allosteric interactions are an important contributor to the catalytic properties of enzyme. Here the authors demonstrate—using the prototypical protein kinase PKA—that the allosteric cooperativity underscoring substrate recognition and product release are directly linked to changes in conformational entropy.

    • Yingjie Wang
    • , Manu V.S.
    • , Jonggul Kim
    • , Geoffrey Li
    • , Lalima G. Ahuja
    • , Philip Aoto
    • , Susan S. Taylor
    •  & Gianluigi Veglia
  • Research | | open

    Bacterial O antigen polysaccharides play a role in innate immune response evasion. Here, the authors uncover the ATP-bound structure of the Aquifex aeolicus WzmWzt O antigen ABC transporter, shedding light onto the mechanism of lipid-linked polysaccharide translocation.

    • Christopher A. Caffalette
    • , Robin A. Corey
    • , Mark S. P. Sansom
    • , Phillip J. Stansfeld
    •  & Jochen Zimmer
  • Research | | open

    SecB homologs can be associated with stress-responsive type II toxin–antitoxin (TA) systems and form tripartite toxin-antitoxin-chaperone systems (TAC). Here the authors provide structural insights into TACs by presenting the crystal structure of the M. tuberculosis TA-associated SecB chaperone in complex with the C-terminal ChAD (chaperone addiction) extension of the antitoxin HigA1.

    • Valérie Guillet
    • , Patricia Bordes
    • , Cécile Bon
    • , Julien Marcoux
    • , Virginie Gervais
    • , Ambre Julie Sala
    • , Suzana Dos Reis
    • , Nawel Slama
    • , Israel Mares-Mejía
    • , Anne-Marie Cirinesi
    • , Laurent Maveyraud
    • , Pierre Genevaux
    •  & Lionel Mourey
  • Research | | open

    Serine/threonine phosphatases such as PP1 associate with a large array of subunit proteins, such as ASPP (apoptosis-stimulating protein of p53) to achieve selective targeting. Here authors solved the crystal structure of the human ASPP2/PP1 complex and explain how ASPP2 can distinguish between PP1 isoforms.

    • M. Teresa Bertran
    • , Stéphane Mouilleron
    • , Yanxiang Zhou
    • , Rakhi Bajaj
    • , Federico Uliana
    • , Ganesan Senthil Kumar
    • , Audrey van Drogen
    • , Rebecca Lee
    • , Jennifer J. Banerjee
    • , Simon Hauri
    • , Nicola O’Reilly
    • , Matthias Gstaiger
    • , Rebecca Page
    • , Wolfgang Peti
    •  & Nicolas Tapon
  • Research | | open

    The fusion peptide (FP) of HIV envelope (Env) is critical in the cell entry process. Here, Kumar et al. present crystal structures of B41 SOSIP.664 Env trimer and show the dynamic nature of the FP and proximal region, which likely relates to conformational rearrangements required for membrane fusion.

    • Sonu Kumar
    • , Anita Sarkar
    • , Pavel Pugach
    • , Rogier W. Sanders
    • , John P. Moore
    • , Andrew B. Ward
    •  & Ian A. Wilson

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