Structure determination

Definition

Structure determination is a procedure by which the three-dimensional atomic coordinates of a molecule or biomolecule are solved using an analytical technique. Many techniques are used in structure determination, most commonly X-ray crystallography, NMR spectroscopy, electron microscopy and molecular modelling.

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Latest Research and Reviews

  • Research |

    A high-resolution structure of the human ribosome determined by cryo-electron microscopy visualizes numerous RNA modifications that are concentrated at functional sites with an extended shell, and suggests the possibility of designing more specific ribosome-targeting drugs.

    • S. Kundhavai Natchiar
    • , Alexander G. Myasnikov
    • , Hanna Kratzat
    • , Isabelle Hazemann
    •  & Bruno P. Klaholz
  • Research | | open

    Neutralizing antibodies to oligomannose glycans on HIV Env are difficult to elicit, possibly due to B cell tolerance. Here, Pantophlet et al. synthesize mimetics based on a bacterial oligosaccharide and show that they evoke HIV-neutralizing antibody responses in animals with a human Ig repertoire.

    • Ralph Pantophlet
    • , Nino Trattnig
    • , Sasha Murrell
    • , Naiomi Lu
    • , Dennis Chau
    • , Caitlin Rempel
    • , Ian A. Wilson
    •  & Paul Kosma
  • Research | | open

    Leishmaniasis is a parasitic disease transmitted by the bite of infected sand flies. Here the authors describe an atomic resolution cryo-EM structure of the Leishmania ribosome in complex with the recently approved drug paromomycin (PAR) and highlight conserved elements in the drug binding pocket that mediate PAR deleterious effects on the parasite.

    • Moran Shalev-Benami
    • , Yan Zhang
    • , Haim Rozenberg
    • , Yuko Nobe
    • , Masato Taoka
    • , Donna Matzov
    • , Ella Zimmerman
    • , Anat Bashan
    • , Toshiaki Isobe
    • , Charles L. Jaffe
    • , Ada Yonath
    •  & Georgios Skiniotis
  • Research | | open

    Using photosensitive caged-compound for femtosecond crystallography at X-ray free electron lasers would allow the structure determination of reaction intermediates. Here the authors demonstrate the feasibility of this approach with a caged NO-compound and present the initial NO-bound intermediate structure of cytochrome P450 nitric oxide reductase.

    • Takehiko Tosha
    • , Takashi Nomura
    • , Takuma Nishida
    • , Naoya Saeki
    • , Kouta Okubayashi
    • , Raika Yamagiwa
    • , Michihiro Sugahara
    • , Takanori Nakane
    • , Keitaro Yamashita
    • , Kunio Hirata
    • , Go Ueno
    • , Tetsunari Kimura
    • , Tamao Hisano
    • , Kazumasa Muramoto
    • , Hitomi Sawai
    • , Hanae Takeda
    • , Eiichi Mizohata
    • , Ayumi Yamashita
    • , Yusuke Kanematsu
    • , Yu Takano
    • , Eriko Nango
    • , Rie Tanaka
    • , Osamu Nureki
    • , Osami Shoji
    • , Yuka Ikemoto
    • , Hironori Murakami
    • , Shigeki Owada
    • , Kensuke Tono
    • , Makina Yabashi
    • , Masaki Yamamoto
    • , Hideo Ago
    • , So Iwata
    • , Hiroshi Sugimoto
    • , Yoshitsugu Shiro
    •  & Minoru Kubo
  • Research | | open

    Plasmodium falciparum protein Pfs25 is a promising malaria transmission blocking vaccine antigen. Here, Scally et al. determine the crystal structure of Pfs25 and identify antigenic sites that are recognized by transmission-blocking antibodies elicited in human immunoglobulin loci transgenic mice.

    • Stephen W. Scally
    • , Brandon McLeod
    • , Alexandre Bosch
    • , Kazutoyo Miura
    • , Qi Liang
    • , Sean Carroll
    • , Sini Reponen
    • , Ngan Nguyen
    • , Eldar Giladi
    • , Sebastian Rämisch
    • , Vidadi Yusibov
    • , Allan Bradley
    • , Franck Lemiale
    • , William R. Schief
    • , Daniel Emerling
    • , Paul Kellam
    • , C. Richter King
    •  & Jean-Philippe Julien

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