Structural biology

  • Article
    | Open Access

    The Lys48-linked polyubiquitin-mediated proteasomal degradation in yeast depends on Cdc48 and its cofactors Ufd1 and Npl4. Here, the authors present crystal structures of Npl4 bound to Lys48-linked diubiquitin and the Npl4-binding motif of Ufd1, providing insights into the reaction mechanism of the Cdc48- Ufd1/Npl4 complex.

    • Yusuke Sato
    • , Hikaru Tsuchiya
    • , Atsushi Yamagata
    • , Kei Okatsu
    • , Keiji Tanaka
    • , Yasushi Saeki
    •  & Shuya Fukai
  • Article
    | Open Access

    The design of protein assemblies is a major thrust for biomolecular engineering and nanobiotechnology. Here the authors demonstrate a general mechanism for designing allosteric macromolecular assemblies and showcase a proof of concept for engineered allosteric protein assembly.

    • Luis A. Campos
    • , Rajendra Sharma
    • , Sara Alvira
    • , Federico M. Ruiz
    • , Beatriz Ibarra-Molero
    • , Mourad Sadqi
    • , Carlos Alfonso
    • , Germán Rivas
    • , Jose M. Sanchez-Ruiz
    • , Antonio Romero Garrido
    • , José M. Valpuesta
    •  & Victor Muñoz
  • Article
    | Open Access

    Myeloid-derived growth factor (MYDGF) is an endoplasmic reticulum protein of therapeutic interest because it promotes tissue repair in a murine model of myocardial infarction. Here the authors present the NMR structure of human MYDGF and attribute function to a set of residues conserved in MYDGFs but not the vanin base domain, which has a similar fold.

    • Valeriu Bortnov
    • , Marco Tonelli
    • , Woonghee Lee
    • , Ziqing Lin
    • , Douglas S. Annis
    • , Omar N. Demerdash
    • , Alex Bateman
    • , Julie C. Mitchell
    • , Ying Ge
    • , John L. Markley
    •  & Deane F. Mosher
  • Article
    | Open Access

    Cysteinyl leukotriene G protein-coupled receptors CysLT1 and CysLT2 regulate pro-inflammatory responses associated with allergic disorders. Here, authors describe four crystal structures of CysLT2R in complex with three dual CysLT1R/CysLT2R antagonists, which shed light on CysLTR ligand selectivity.

    • Anastasiia Gusach
    • , Aleksandra Luginina
    • , Egor Marin
    • , Rebecca L. Brouillette
    • , Élie Besserer-Offroy
    • , Jean-Michel Longpré
    • , Andrii Ishchenko
    • , Petr Popov
    • , Nilkanth Patel
    • , Taku Fujimoto
    • , Toru Maruyama
    • , Benjamin Stauch
    • , Margarita Ergasheva
    • , Daria Romanovskaia
    • , Anastasiia Stepko
    • , Kirill Kovalev
    • , Mikhail Shevtsov
    • , Valentin Gordeliy
    • , Gye Won Han
    • , Vsevolod Katritch
    • , Valentin Borshchevskiy
    • , Philippe Sarret
    • , Alexey Mishin
    •  & Vadim Cherezov
  • Article
    | Open Access

    SPOR domains are present in bacterial proteins that recognize cell-wall peptidoglycan strands stripped of the peptide stems. Here, Alcorlo et al. show that, indeed, the presence of peptide stems abrogates binding to a SPOR domain, and provide insights into the molecular basis for recognition.

    • Martín Alcorlo
    • , David A. Dik
    • , Stefania De Benedetti
    • , Kiran V. Mahasenan
    • , Mijoon Lee
    • , Teresa Domínguez-Gil
    • , Dusan Hesek
    • , Elena Lastochkin
    • , Daniel López
    • , Bill Boggess
    • , Shahriar Mobashery
    •  & Juan A. Hermoso
  • Article
    | Open Access

    MLL family histone methyltransferases deposit histone H3 Lys4 mono-/di-/tri-methylation and regulate gene expression in mammals. Here the authors report the single-particle cryo-EM structure of the NCP-bound human MLL1 core complex, shedding light on how the MLL1 complex engages chromatin and how chromatin binding promotes MLL1 tri-methylation activity.

    • Sang Ho Park
    • , Alex Ayoub
    • , Young-Tae Lee
    • , Jing Xu
    • , Hanseong Kim
    • , Wei Zheng
    • , Biao Zhang
    • , Liang Sha
    • , Sojin An
    • , Yang Zhang
    • , Michael A. Cianfrocco
    • , Min Su
    • , Yali Dou
    •  & Uhn-Soo Cho
  • Article
    | Open Access

    RNA polymerase I (Pol I) catalyses the transcription of pre-ribosomal RNA and for transcription initiation Pol I assembles with core factor and Rrn3 on the rDNA core promoter. Here the authors provide insights into the molecular mechanism of promoter opening by Pol I by determining the cryo-EM structures of two closed, two intermediate and two open Pol I pre-initiation complexes.

    • Yashar Sadian
    • , Florence Baudin
    • , Lucas Tafur
    • , Brice Murciano
    • , Rene Wetzel
    • , Felix Weis
    •  & Christoph W. Müller
  • Article
    | Open Access

    Parkinson’s disease (PD) and Multiple System Atrophy (MSA) are characterized by the pathological accumulation of α-synuclein. Here the authors employ fluorescent probes, electron microscopy and NMR spectroscopy to study the properties of α-synuclein aggregates that were amplified from patient brain extracts and observe a greater structural diversity among PD patients compared to MSA patients.

    • Timo Strohäker
    • , Byung Chul Jung
    • , Shu-Hao Liou
    • , Claudio O. Fernandez
    • , Dietmar Riedel
    • , Stefan Becker
    • , Glenda M. Halliday
    • , Marina Bennati
    • , Woojin S. Kim
    • , Seung-Jae Lee
    •  & Markus Zweckstetter
  • Article
    | Open Access

    The conformational heterogeneity of RNA molecules makes their structure determination by X-ray crystallography and NMR challenging. Here the authors show that RNA structures can be solved by cryo-EM and present the structures of a 40 kDa SAM-IV riboswitch in the apo form and bound to its ligand S-adenosylmethionine.

    • Kaiming Zhang
    • , Shanshan Li
    • , Kalli Kappel
    • , Grigore Pintilie
    • , Zhaoming Su
    • , Tung-Chung Mou
    • , Michael F. Schmid
    • , Rhiju Das
    •  & Wah Chiu
  • Article
    | Open Access

    The activation mechanism of integrin αVβ8 differs from other integrins. Combining X-ray crystallography, hydrogen deuterium exchange mass spectrometry and mutation, the authors reveal structural features responsible for these differences and provide insights into how typical integrins are regulated.

    • Jianchuan Wang
    • , Yang Su
    • , Roxana E. Iacob
    • , John R. Engen
    •  & Timothy A. Springer
  • Article
    | Open Access

    DNA ligases catalyze the joining of DNA strands to complete DNA replication, recombination and repair transactions. Here the authors present X-ray structures and kinetic analyses of LIG1 complexes with undamaged and oxidatively damaged DNA that unveil determinants of LIG1 substrate recognition and enzymatic fidelity.

    • Percy P. Tumbale
    • , Thomas J. Jurkiw
    • , Matthew J. Schellenberg
    • , Amanda A. Riccio
    • , Patrick J O’Brien
    •  & R. Scott Williams
  • Article
    | Open Access

    Bacterial type II secretion systems (T2SSs) translocate virulence factors, toxins and enzymes across the cell outer membrane. Here, Chernyatina and Low use negative stain and cryo-electron microscopy to reveal the core architecture of an assembled T2SS from the pathogen Klebsiella pneumoniae.

    • Anastasia A. Chernyatina
    •  & Harry H. Low
  • Article
    | Open Access

    Polar-faceted nanocrystals often have complex atomic surface configurations that are very difficult to characterize. Here, the authors are able to determine detailed structural information about the polar facets of ceria nanocubes by using 17O and 1H solid-state NMR spectroscopy, in combination with density functional theory calculations.

    • Junchao Chen
    • , Xin-Ping Wu
    • , Michael A. Hope
    • , Kun Qian
    • , David M. Halat
    • , Tao Liu
    • , Yuhong Li
    • , Li Shen
    • , Xiaokang Ke
    • , Yujie Wen
    • , Jia-Huan Du
    • , Pieter C. M. M. Magusin
    • , Subhradip Paul
    • , Weiping Ding
    • , Xue-Qing Gong
    • , Clare P. Grey
    •  & Luming Peng
  • Article
    | Open Access

    Cytotoxic T lymphocytes (CTLs) eliminate virus-infected and cancerous cells by secreting the pore-forming protein (perforin) and pro-apoptotic serine proteases (granzymes). Here authors show that two mechanisms protect the membranes of CTLs from disruption by perforin and granzymes.

    • Jesse A. Rudd-Schmidt
    • , Adrian W. Hodel
    • , Tahereh Noori
    • , Jamie A. Lopez
    • , Hyun-Jung Cho
    • , Sandra Verschoor
    • , Annette Ciccone
    • , Joseph A. Trapani
    • , Bart W. Hoogenboom
    •  & Ilia Voskoboinik
  • Article
    | Open Access

    Myeloid-derived growth factor (MYDGF) is an angiogenic growth factor with therapeutic potential for ischemic tissue repair and the receptor is still unknown. Here the authors present the crystal structure of human MYDGF and identify its functional epitope through mutagenesis studies.

    • Rebecca Ebenhoch
    • , Abbas Akhdar
    • , Marc R. Reboll
    • , Mortimer Korf-Klingebiel
    • , Priyanka Gupta
    • , Julie Armstrong
    • , Yining Huang
    • , Lee Frego
    • , Irina Rybina
    • , John Miglietta
    • , Anton Pekcec
    • , Kai C. Wollert
    •  & Herbert Nar
  • Article
    | Open Access

    Here, the authors identify a broadly neutralizing antibody from an HIV-infected person that recognizes the membrane-proximal external region (MPER) of HIV envelope glycoprotein (Env) and has a short CDRH3 and low polyreactivity. Structural analysis shows how the antibody binds the MPER and Env on the viral membrane.

    • Lei Zhang
    • , Adriana Irimia
    • , Lingling He
    • , Elise Landais
    • , Kimmo Rantalainen
    • , Daniel P. Leaman
    • , Thomas Vollbrecht
    • , Armando Stano
    • , Daniel I. Sands
    • , Arthur S. Kim
    • , George Miiro
    • , Jennifer Serwanga
    • , Anton Pozniak
    • , Dale McPhee
    • , Oliver Manigart
    • , Lawrence Mwananyanda
    • , Etienne Karita
    • , André Inwoley
    • , Walter Jaoko
    • , Jack DeHovitz
    • , Linda-Gail Bekker
    • , Punnee Pitisuttithum
    • , Robert Paris
    • , Susan Allen
    • , Pascal Poignard
    • , Dennis R. Burton
    • , Ben Murrell
    • , Andrew B. Ward
    • , Jiang Zhu
    • , Ian A. Wilson
    •  & Michael B. Zwick
  • Article
    | Open Access

    Pif1 plays multiple roles in maintaining genome stability and preferentially unwinds forked dsDNA. Here the authors solve the structure of Bacteroides sp Pif1 (BaPif1) in complex with a symmetrical double forked dsDNA and provide unprecedented insights into forked dsDNA unwinding by BaPif1.

    • Nannan Su
    • , Alicia K. Byrd
    • , Sakshibeedu R. Bharath
    • , Olivia Yang
    • , Yu Jia
    • , Xuhua Tang
    • , Taekjip Ha
    • , Kevin D. Raney
    •  & Haiwei Song
  • Article
    | Open Access

    Human Herpesvirus 6B (HHV-6) can cause fever, diarrhea and roseola rash. Here the authors present a cryoEM approach to image crude, minimally purified virus samples and employ it to determine the atomic structures of HHV-6B capsid and capsid-associated tegument protein complexes.

    • Yibo Zhang
    • , Wei Liu
    • , Zihang Li
    • , Vinay Kumar
    • , Ana L. Alvarez-Cabrera
    • , Emily C. Leibovitch
    • , Yanxiang Cui
    • , Ye Mei
    • , Guo-Qiang Bi
    • , Steve Jacobson
    •  & Z. Hong Zhou
  • Article
    | Open Access

    The bacterial flagellar hook is a molecular universal joint that connects the rotary motor and long helical propeller of the bacterial flagellum. Here the authors present the 3.6 Å resolution cryo-EM structure of the native supercoiled Salmonella hook that provides insights into the dynamic changes of subunit conformations and intermolecular interactions of the hook protein FlgE.

    • Takayuki Kato
    • , Fumiaki Makino
    • , Tomoko Miyata
    • , Péter Horváth
    •  & Keiichi Namba
  • Article
    | Open Access

    In Myxococcus xanthus, directed movement is controlled by pole-to-pole oscillations of the small GTPase MglA and its GAP MglB. Here authors report the complete MglA/MglB structural cycle and uncover that MglA is a three-state GTPase that adopts an atypical GTP-bound state that is refractory to inactivation by MglB.

    • Christian Galicia
    • , Sébastien Lhospice
    • , Paloma Fernández Varela
    • , Stefano Trapani
    • , Wenhua Zhang
    • , Jorge Navaza
    • , Julien Herrou
    • , Tâm Mignot
    •  & Jacqueline Cherfils
  • Article
    | Open Access

    The cofilin family proteins are actin disassembly factors but the disassembly mechanism is poorly understood. Here authors show that cyclase-associated-protein (CAP) works in synergy with cofilin to accelerate actin filament depolymerization by nearly 100-fold and reveal how CAP destabilizes the interface between terminal actin subunits.

    • Tommi Kotila
    • , Hugo Wioland
    • , Giray Enkavi
    • , Konstantin Kogan
    • , Ilpo Vattulainen
    • , Antoine Jégou
    • , Guillaume Romet-Lemonne
    •  & Pekka Lappalainen
  • Article
    | Open Access

    CKK domain containing CAMSAP/Patronins recognise and regulate microtubule (MT) minus end dynamics. Here the authors compare cryo-EM structures of MT-bound human CKK and Naegleria gruberi CKK which lacks minus-end binding preference, finding NgCKK has a different interaction with, and inability to remodel, its MT binding site, shedding light on the CAMSAP/Patronin end binding mechanism.

    • Joseph Atherton
    • , Yanzhang Luo
    • , Shengqi Xiang
    • , Chao Yang
    • , Ankit Rai
    • , Kai Jiang
    • , Marcel Stangier
    • , Annapurna Vemu
    • , Alexander D. Cook
    • , Su Wang
    • , Antonina Roll-Mecak
    • , Michel O. Steinmetz
    • , Anna Akhmanova
    • , Marc Baldus
    •  & Carolyn A. Moores
  • Article
    | Open Access

    Natural killer T (NKT) cells include type I that express semi-invariant T cell receptor (TCR), and type II that cover a broader repertoire. Here the authors describe the crystal structure of a type II NKT TCR complexed with CD1d/antigen to propose that type II NKT TCRs may adapt multiple CD1d docking modes to maximise antigen recognition efficacy.

    • Catarina F. Almeida
    • , Srinivasan Sundararaj
    • , Jérôme Le Nours
    • , T. Praveena
    • , Benjamin Cao
    • , Satvika Burugupalli
    • , Dylan G. M. Smith
    • , Onisha Patel
    • , Manfred Brigl
    • , Daniel G. Pellicci
    • , Spencer J. Williams
    • , Adam P. Uldrich
    • , Dale I. Godfrey
    •  & Jamie Rossjohn
  • Article
    | Open Access

    Tc toxins are a major class of bacterial toxin translocation systems that inject toxic enzymes into target cells. Here the authors present functional and structural data showing that the toxic enzyme can be replaced by other small proteins and identify prerequisites required for successful translocation, which could facilitate the development of functional Tc-based protein injection devices.

    • Daniel Roderer
    • , Evelyn Schubert
    • , Oleg Sitsel
    •  & Stefan Raunser
  • Article
    | Open Access

    Bacterial type IV pilus-like systems catalyse the formation of pilin fibres but it is unknown how they are powered. Here, the authors present crystal and cryo-EM structures of the hexameric motor ATPases PilB and PilT from Type IVa Pilus that reveal different conformational states, classify the conformations of all PilT-like ATPase structures and propose a model for PilT function.

    • Matthew McCallum
    • , Samir Benlekbir
    • , Sheryl Nguyen
    • , Stephanie Tammam
    • , John L. Rubinstein
    • , Lori L. Burrows
    •  & P. Lynne Howell
  • Article
    | Open Access

    Nuclear spins in diamond have applications in quantum technologies and NMR methods but their performance can be limited by relaxation processes that are difficult to characterise. Ajoy et al. develop a T1 noise spectroscopy method to identify the dominant relaxation channel and propose a mitigation strategy.

    • A. Ajoy
    • , B. Safvati
    • , R. Nazaryan
    • , J. T. Oon
    • , B. Han
    • , P. Raghavan
    • , R. Nirodi
    • , A. Aguilar
    • , K. Liu
    • , X. Cai
    • , X. Lv
    • , E. Druga
    • , C. Ramanathan
    • , J. A. Reimer
    • , C. A. Meriles
    • , D. Suter
    •  & A. Pines
  • Article
    | Open Access

    Cytochrome bd oxidases couple quinol oxidation and the release of protons to the periplasmic side with proton uptake from the cytoplasmic side to reduce dioxygen to water and they are the terminal reductases in bacterial and archaeal respiratory chains. Here the authors present the cryo-EM structure of Escherichia coli bd oxidase and discuss mechanistic implications.

    • Alexander Theßeling
    • , Tim Rasmussen
    • , Sabrina Burschel
    • , Daniel Wohlwend
    • , Jan Kägi
    • , Rolf Müller
    • , Bettina Böttcher
    •  & Thorsten Friedrich
  • Article
    | Open Access

    The polarisome is a dynamic protein complex that nucleates F-actin for polarized yeast growth, but its regulation is unclear. Here, the authors report that the polarisome protein Aip5 undergoes Spa2-mediated phase separation in physiological and stress conditions, potentially for regulating actin assembly.

    • Ying Xie
    • , Jialin Sun
    • , Xiao Han
    • , Alma Turšić-Wunder
    • , Joel D. W. Toh
    • , Wanjin Hong
    • , Yong-Gui Gao
    •  & Yansong Miao
  • Article
    | Open Access

    The Staufen proteins recognize secondary structures in 3’-untranslated regions in mRNA transcripts and induce degradation of these mRNAs with the help of the RNA helicase UPF1. Here the authors report that the nonsense-mediated mRNA decay factor UPF2 mediates the interaction between Stau1 and UPF1 in Staufen-mediated mRNA decay.

    • Manjeera Gowravaram
    • , Juliane Schwarz
    • , Sana K. Khilji
    • , Henning Urlaub
    •  & Sutapa Chakrabarti
  • Article
    | Open Access

    The N6-methyladenosine (m6A) RNA modification is an evolutionarily conserved epitranscriptomic mechanism that impacts several cellular processes. Here the authors present a structure-function analysis of the ZCCHC4, 28S RNA-specific m6A methyltransferase, shedding light on its regulation and mechanisms that ensure substrate specificity.

    • Wendan Ren
    • , Jiuwei Lu
    • , Mengjiang Huang
    • , Linfeng Gao
    • , Dongxu Li
    • , Gang Greg Wang
    •  & Jikui Song
  • Article
    | Open Access

    The European X-ray free-electron laser (EuXFEL) in Hamburg is the first XFEL with a megahertz repetition rate. Here the authors present the 2.9 Å structure of the large membrane protein complex Photosystem I from T. elongatus that was determined at the EuXFEL.

    • Chris Gisriel
    • , Jesse Coe
    • , Romain Letrun
    • , Oleksandr M. Yefanov
    • , Cesar Luna-Chavez
    • , Natasha E. Stander
    • , Stella Lisova
    • , Valerio Mariani
    • , Manuela Kuhn
    • , Steve Aplin
    • , Thomas D. Grant
    • , Katerina Dörner
    • , Tokushi Sato
    • , Austin Echelmeier
    • , Jorvani Cruz Villarreal
    • , Mark S. Hunter
    • , Max O. Wiedorn
    • , Juraj Knoska
    • , Victoria Mazalova
    • , Shatabdi Roy-Chowdhury
    • , Jay-How Yang
    • , Alex Jones
    • , Richard Bean
    • , Johan Bielecki
    • , Yoonhee Kim
    • , Grant Mills
    • , Britta Weinhausen
    • , Jose D. Meza
    • , Nasser Al-Qudami
    • , Saša Bajt
    • , Gerrit Brehm
    • , Sabine Botha
    • , Djelloul Boukhelef
    • , Sandor Brockhauser
    • , Barry D. Bruce
    • , Matthew A. Coleman
    • , Cyril Danilevski
    • , Erin Discianno
    • , Zachary Dobson
    • , Hans Fangohr
    • , Jose M. Martin-Garcia
    • , Yaroslav Gevorkov
    • , Steffen Hauf
    • , Ahmad Hosseinizadeh
    • , Friederike Januschek
    • , Gihan K. Ketawala
    • , Christopher Kupitz
    • , Luis Maia
    • , Maurizio Manetti
    • , Marc Messerschmidt
    • , Thomas Michelat
    • , Jyotirmoy Mondal
    • , Abbas Ourmazd
    • , Gianpietro Previtali
    • , Iosifina Sarrou
    • , Silvan Schön
    • , Peter Schwander
    • , Megan L. Shelby
    • , Alessandro Silenzi
    • , Jolanta Sztuk-Dambietz
    • , Janusz Szuba
    • , Monica Turcato
    • , Thomas A. White
    • , Krzysztof Wrona
    • , Chen Xu
    • , Mohamed H. Abdellatif
    • , James D. Zook
    • , John C. H. Spence
    • , Henry N. Chapman
    • , Anton Barty
    • , Richard A. Kirian
    • , Matthias Frank
    • , Alexandra Ros
    • , Marius Schmidt
    • , Raimund Fromme
    • , Adrian P. Mancuso
    • , Petra Fromme
    •  & Nadia A. Zatsepin
  • Article
    | Open Access

    Systemic amyloidosis of the ATTR is one of the most abundant forms of systemic amyloidosis and caused by misfolding of the circulating blood protein transthyretin (TTR). Here the authors present the cryo-EM structure of patient-derived Val30Met ATTR amyloid fibrils which reveals that the protofilament consists of an N-terminal and a C-terminal TTR fragment and discuss implications for the mechanism of misfolding.

    • Matthias Schmidt
    • , Sebastian Wiese
    • , Volkan Adak
    • , Jonas Engler
    • , Shubhangi Agarwal
    • , Günter Fritz
    • , Per Westermark
    • , Martin Zacharias
    •  & Marcus Fändrich
  • Article
    | Open Access

    A membrane-exposed groove in Ca2+-gated TMEM16 scramblases forms the translocation pathway for ions and lipids. Here authors combine molecular dynamics with cryo-EM and functional assays to uncover the conformational transitions of the groove leading to a non-selective ion channel pore.

    • George Khelashvili
    • , Maria E. Falzone
    • , Xiaolu Cheng
    • , Byoung-Cheol Lee
    • , Alessio Accardi
    •  & Harel Weinstein
  • Article
    | Open Access

    Regulation of centriole duplication is essential for normal chromosome segregation and the maintenance of genomic stability, and Polo-like kinase 4 (Plk4) is a known regulator with unclear mechanisms. Here the authors show that Plk4 undergoes phase separation into an assembling body through autophosphorylation which drives centriole biogenesis.

    • Jung-Eun Park
    • , Liang Zhang
    • , Jeong Kyu Bang
    • , Thorkell Andresson
    • , Frank DiMaio
    •  & Kyung S. Lee
  • Article
    | Open Access

    Photosystem I (PSI) is embedded in thylakoid membranes of photosynthetic organisms, converting light energy into chemical energy, and its oligomeric state varies among different organisms. Here the authors present the 3.3 Å resolution cryo-EM structure of the PSI tetramer from the cyanobacterium Anabaena sp. PCC 7120.

    • Koji Kato
    • , Ryo Nagao
    • , Tian-Yi Jiang
    • , Yoshifumi Ueno
    • , Makio Yokono
    • , Siu Kit Chan
    • , Mai Watanabe
    • , Masahiko Ikeuchi
    • , Jian-Ren Shen
    • , Seiji Akimoto
    • , Naoyuki Miyazaki
    •  & Fusamichi Akita
  • Article
    | Open Access

    Bacterial DNA gyrase is the only type II DNA topoisomerase capable of introducing negative supercoils into DNA and is of interest as a drug target. Here the authors present the cryo-EM structure of the complete E. coli DNA gyrase bound to a 180 bp double-stranded DNA and the antibiotic gepotidacin, which reveals the connections between the functional domains and their spatial organization.

    • Arnaud Vanden Broeck
    • , Christophe Lotz
    • , Julio Ortiz
    •  & Valérie Lamour
  • Article
    | Open Access

    Rhodopsin genes have been identified in some large double-stranded DNA viruses, but the structure and functions of viral rhodopsins remain unknown. Here authors present crystal structure and characterization of an Organic Lake Phycodnavirus rhodopsin II (OLPVRII) which forms a pentamer and is a weak proton pump.

    • Dmitry Bratanov
    • , Kirill Kovalev
    • , Jan-Philipp Machtens
    • , Roman Astashkin
    • , Igor Chizhov
    • , Dmytro Soloviov
    • , Dmytro Volkov
    • , Vitaly Polovinkin
    • , Dmitrii Zabelskii
    • , Thomas Mager
    • , Ivan Gushchin
    • , Tatyana Rokitskaya
    • , Yuri Antonenko
    • , Alexey Alekseev
    • , Vitaly Shevchenko
    • , Natalya Yutin
    • , Riccardo Rosselli
    • , Christian Baeken
    • , Valentin Borshchevskiy
    • , Gleb Bourenkov
    • , Alexander Popov
    • , Taras Balandin
    • , Georg Büldt
    • , Dietmar J. Manstein
    • , Francisco Rodriguez-Valera
    • , Christoph Fahlke
    • , Ernst Bamberg
    • , Eugene Koonin
    •  & Valentin Gordeliy
  • Article
    | Open Access

    Alzheimer’s disease is characterised by the deposition of Aβ amyloid fibrils and tau protein neurofibrillary tangles. Here the authors use cryo-EM to structurally characterise brain derived Aβ amyloid fibrils and find that they are polymorphic and right-hand twisted, which differs from in vitro generated Aβ fibrils.

    • Marius Kollmer
    • , William Close
    • , Leonie Funk
    • , Jay Rasmussen
    • , Aref Bsoul
    • , Angelika Schierhorn
    • , Matthias Schmidt
    • , Christina J. Sigurdson
    • , Mathias Jucker
    •  & Marcus Fändrich
  • Article
    | Open Access

    Mitofusin-2 (MFN2) is a dynamin-like GTPase that plays a central role in regulating mitochondrial fusion and cell metabolism. Here, authors report crystal structures of truncated human MFN2 in different nucleotide-loading states and show that MFN2 forms sustained dimers even after GTP hydrolysis.

    • Yu-Jie Li
    • , Yu-Lu Cao
    • , Jian-Xiong Feng
    • , Yuanbo Qi
    • , Shuxia Meng
    • , Jie-Feng Yang
    • , Ya-Ting Zhong
    • , Sisi Kang
    • , Xiaoxue Chen
    • , Lan Lan
    • , Li Luo
    • , Bing Yu
    • , Shoudeng Chen
    • , David C. Chan
    • , Junjie Hu
    •  & Song Gao
  • Article
    | Open Access

    Aerolysin pores have potential to improve the accuracy of DNA sequencing and single-molecule proteomics. Here, the authors rationally design a set of mutated pores to achieve a more accurate detection of peptides and nucleic acids.

    • Chan Cao
    • , Nuria Cirauqui
    • , Maria Jose Marcaida
    • , Elena Buglakova
    • , Alice Duperrex
    • , Aleksandra Radenovic
    •  & Matteo Dal Peraro
  • Article
    | Open Access

    The structures and dynamics of large proteins can be studied with methyl-based NMR but peak assignment is still challenging. Here the authors present MethylFLYA that allows automated assignment of methyl groups and apply it to five proteins with molecular weights in the range from 28 to 358 kDa.

    • Iva Pritišanac
    • , Julia M. Würz
    • , T. Reid Alderson
    •  & Peter Güntert
  • Article
    | Open Access

    AspH catalyses hydroxylation of asparagine and aspartate residues in epidermal growth factor-like domains (EGFDs). Here, the authors present crystal structures of AspH with and without substrates and show that AspH uses EFGD substrates with a non-canonical disulfide pattern.

    • Inga Pfeffer
    • , Lennart Brewitz
    • , Tobias Krojer
    • , Sacha A. Jensen
    • , Grazyna T. Kochan
    • , Nadia J. Kershaw
    • , Kirsty S. Hewitson
    • , Luke A. McNeill
    • , Holger Kramer
    • , Martin Münzel
    • , Richard J. Hopkinson
    • , Udo Oppermann
    • , Penny A. Handford
    • , Michael A. McDonough
    •  & Christopher J. Schofield
  • Article
    | Open Access

    Viral assembly is a complex process that in tailed bacteriophages involves scaffolding proteins which coordinate assembly of the phage procapsid and are subsequently released during maturation. Here the authors reveal the conformational changes that accompany virion maturation, documenting how the dissociation of scaffold proteins and DNA packaging processes intersect.

    • Athanasios Ignatiou
    • , Sandrine Brasilès
    • , Mehdi El Sadek Fadel
    • , Jörg Bürger
    • , Thorsten Mielke
    • , Maya Topf
    • , Paulo Tavares
    •  & Elena V. Orlova
  • Article
    | Open Access

    Metal-mediated base pairs expand the repertoire of nucleic acid structures and dynamics. Here, the authors prepared a metallo-DNA duplex including two C-Hg(II)-T base pairs separated by six normal Watson-Crick base pairs and investigated its solution structure and dynamics using NMR spectroscopy.

    • Olivia P. Schmidt
    • , Simon Jurt
    • , Silke Johannsen
    • , Ashkan Karimi
    • , Roland K. O. Sigel
    •  & Nathan W. Luedtke
  • Article
    | Open Access

    Sialidases are glycoside hydrolases that cleave sialosides. Here the authors define the 3-D structure, alone and in complex with products and inhibitors, of the CAZy family GH156 sialidase, EnvSia156, showing it displays a catalytical (β/α) 8-barrel domain distinct from other sialidases and allowing description of its inverting catalytic mechanism.

    • Pedro Bule
    • , Léa Chuzel
    • , Elena Blagova
    • , Liang Wu
    • , Melissa A. Gray
    • , Bernard Henrissat
    • , Erdmann Rapp
    • , Carolyn R. Bertozzi
    • , Christopher H. Taron
    •  & Gideon J. Davies
  • Article
    | Open Access

    Myosin, a motor protein essential for intracellular transport to muscle contraction, requires a chaperone UNC-45 for folding and assembly. Here authors use in vitro reconstitution and structural biology to characterize the interplay between UNC-45 and muscle myosin MHC-B.

    • Doris Hellerschmied
    • , Anita Lehner
    • , Nina Franicevic
    • , Renato Arnese
    • , Chloe Johnson
    • , Antonia Vogel
    • , Anton Meinhart
    • , Robert Kurzbauer
    • , Luiza Deszcz
    • , Linn Gazda
    • , Michael Geeves
    •  & Tim Clausen
  • Article
    | Open Access

    The Bin/Amphiphysin/Rvs167 (BAR) domain superfamily, which includes FCH-BAR (F-BAR) domain proteins are membrane-sculpting proteins. Here the authors combine a range of techniques and show that the F-BAR domain of growth-arrest specific protein 7 (GAS7) forms two-dimensional sheets on flat membranes and that these oligomeric assemblies of GAS7 are required for the formation of phagocytic cups in macrophages.

    • Kyoko Hanawa-Suetsugu
    • , Yuzuru Itoh
    • , Maisarah Ab Fatah
    • , Tamako Nishimura
    • , Kazuhiro Takemura
    • , Kohei Takeshita
    • , Satoru Kubota
    • , Naoyuki Miyazaki
    • , Wan Nurul Izzati Wan Mohamad Noor
    • , Takehiko Inaba
    • , Nhung Thi Hong Nguyen
    • , Sayaka Hamada-Nakahara
    • , Kayoko Oono-Yakura
    • , Masashi Tachikawa
    • , Kenji Iwasaki
    • , Daisuke Kohda
    • , Masaki Yamamoto
    • , Akio Kitao
    • , Atsushi Shimada
    •  & Shiro Suetsugu
  • Article
    | Open Access

    Acetylation of histone H3K23 has emerged as an essential posttranslational modification, yet this epigenetic mark remains poorly understood. Here, the authors identify the native MORF complex as a histone H3K23-specific acetyltransferase and show that interaction of the MORF subunit with acylated H3K14 promotes acetylation of H3K23 by this complex to activate transcription.

    • Brianna J. Klein
    • , Suk Min Jang
    • , Catherine Lachance
    • , Wenyi Mi
    • , Jie Lyu
    • , Shun Sakuraba
    • , Krzysztof Krajewski
    • , Wesley W. Wang
    • , Simone Sidoli
    • , Jiuyang Liu
    • , Yi Zhang
    • , Xiaolu Wang
    • , Becka M. Warfield
    • , Andrew J. Kueh
    • , Anne K. Voss
    • , Tim Thomas
    • , Benjamin A. Garcia
    • , Wenshe R. Liu
    • , Brian D. Strahl
    • , Hidetoshi Kono
    • , Wei Li
    • , Xiaobing Shi
    • , Jacques Côté
    •  & Tatiana G. Kutateladze