Single-strand DNA breaks

Definition

A single-strand DNA break arises when only one strand of the DNA duplex is severed. They are typically corrected by excision repair pathways.

Latest Research and Reviews

  • Research | | open

    DNA damage response (DDR) is activated in cardiomyocytes of the failing heart, but the type of DNA damage leading to DDR is unclear. Higo et al. show that in mice heart failure is caused in part by unrepaired DNA single-strand breaks in cardiomyocytes, which activate persistent DDR and trigger an NF-κB-dependent cardiac inflammation.

    • Tomoaki Higo
    • , Atsuhiko T. Naito
    • , Tomokazu Sumida
    • , Masato Shibamoto
    • , Katsuki Okada
    • , Seitaro Nomura
    • , Akito Nakagawa
    • , Toshihiro Yamaguchi
    • , Taku Sakai
    • , Akihito Hashimoto
    • , Yuki Kuramoto
    • , Masamichi Ito
    • , Shungo Hikoso
    • , Hiroshi Akazawa
    • , Jong-Kook Lee
    • , Ichiro Shiojima
    • , Peter J. McKinnon
    • , Yasushi Sakata
    •  & Issei Komuro
  • Research | | open

    The expansion of trinucleotide repeats has been linked to several neurodegenerative disorders. Here, the authors show that the CRISPR-Cas9 nuclease induces both expansions and contractions of the repeat region, whereas the nickase leads predominantly to contractions.

    • Cinzia Cinesi
    • , Lorène Aeschbach
    • , Bin Yang
    •  & Vincent Dion
  • Research | | open

    It is recognized that cellular senescence is triggered by DNA damage as a protective mechanism against tumorigenesis. Here the authors show that DNA single-strand breaks of oxidative origin can induce a transient senescent state followed by the emergence of clonal transformed cells.

    • Joe Nassour
    • , Sébastien Martien
    • , Nathalie Martin
    • , Emeric Deruy
    • , Elisa Tomellini
    • , Nicolas Malaquin
    • , Fatima Bouali
    • , Laure Sabatier
    • , Nicolas Wernert
    • , Sébastien Pinte
    • , Eric Gilson
    • , Albin Pourtier
    • , Olivier Pluquet
    •  & Corinne Abbadie
  • Research |

    In the absence of RNase H2, ribonucleotides incorporated during DNA replication can be processed by Top1. This activity is directed to the nascent leading strand, because gaps in the lagging strand would limit torsional tension.

    • Jessica S Williams
    • , Anders R Clausen
    • , Scott A Lujan
    • , Lisette Marjavaara
    • , Alan B Clark
    • , Peter M Burgers
    • , Andrei Chabes
    •  & Thomas A Kunkel
  • Reviews |

    Many mobile genetic elements, such as transposons, plasmids and viruses, must cleave their own DNA to effect transposition, replication or conjugation. Here, Chandler and colleagues describe the HUH endonucleases, which use a unique mechanism to cleave and rejoin single-stranded DNA in order to mobilize and disseminate such elements.

    • Michael Chandler
    • , Fernando de la Cruz
    • , Fred Dyda
    • , Alison B. Hickman
    • , Gabriel Moncalian
    •  & Bao Ton-Hoang
  • Research |

    A system to reconstitute a collapsed replication fork using Xenopus laevis egg extracts is developed. The study shows that upon fork collapse, DNA Pol epsilon and the GINS complex are uncoupled from the replisome, and their reloading onto DNA requires repair proteins Rad51 and Mre11.

    • Yoshitami Hashimoto
    • , Fabio Puddu
    •  & Vincenzo Costanzo

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