Sensors and probes articles from across Nature Portfolio

Trait correlations impact evolvability as selection on one trait can influence others. Here, the authors examine trait correlation in two proteins, a fluorescent protein & an antibiotic resistance enzyme, observing rapid evolution of trait correlations through changes in the biophysical properties of these proteins.

For accurate single cell analyses of glucose uptake ex vivo and in vivo, the authors developed a method using glucose transporter-dependent import of clickable sugars, coupled with fluorescent post-labeling and multicolor flow cytometry.

Modulating amyloid protein phase separation and fibrilization may help in addressing neurodegenerative diseases. This study demonstrates that halogen-doped graphene quantum dots can modulate these processes in TDP-43 in both nucleus and cytoplasm.

The exceptionally photostable green fluorescent protein StayGold has been monomerized in different laboratories, which has generated three unique monomeric variants that will enable new imaging applications.

Many questions on the activity of the Ras proto-oncogene are unanswered due to the lack of tools for detecting active Ras in living cells. Here, we used protein design and structure prediction algorithms to develop biosensors that detect the activity and environment of endogenous Ras.

Sensing changes in ionic current as barcoded DNA translocates through solid-state nanopores allows the study of how nucleotide sequences alter the DNA-binding specificity of the catalytically inactive Cas9 ribonucleoprotein complex.

Developments in nanopore-based peptide detection and sequencing show promise of a breakthrough.

New twists on established methods and multimodal imaging are poised to bridge gaps between cellular and organismal imaging.