RNA modification

Definition

RNA modifications are changes to the chemical composition of ribonucleic acid (RNA) molecules post-synthesis that have the potential to alter function or stability. An example of RNA modification is the addition of a methylated guanine nucleotide “cap” to the 5’-end of messenger RNAs (mRNAs).

Latest Research and Reviews

  • Research | | open

    N 6-methyladenosine (m6A) is a conserved RNA modification that has recently emerged as an important regulator of messenger RNA processing and activity. Here, the authors provide evidence that m6A pathway facilitates female-specific splicing of Sxl, regulating sex determination in Drosophila.

    • Lijuan Kan
    • , Anya V. Grozhik
    • , Jeffrey Vedanayagam
    • , Deepak P. Patil
    • , Nan Pang
    • , Kok-Seong Lim
    • , Yi-Chun Huang
    • , Brian Joseph
    • , Ching-Jung Lin
    • , Vladimir Despic
    • , Jian Guo
    • , Dong Yan
    • , Shu Kondo
    • , Wu-Min Deng
    • , Peter C. Dedon
    • , Samie R. Jaffrey
    •  & Eric C. Lai
  • Research |

    Microprocessor components Dgcr8 and Drosha associate with transcriptionally active coding and noncoding genes in a Mettl3-dependent manner and, upon temperature stress, relocate to heat-shock genes, where they mark mRNAs for subsequent degradation.

    • Philip Knuckles
    • , Sarah H Carl
    • , Michael Musheev
    • , Christof Niehrs
    • , Alice Wenger
    •  & Marc Bühler
  • Research |

    Methylation at the 6 position of adenosine (m6A) in RNA is rapidly and transiently induced at DNA damage sites in response to ultraviolet irradiation.

    • Yang Xiang
    • , Benoit Laurent
    • , Chih-Hung Hsu
    • , Sigrid Nachtergaele
    • , Zhike Lu
    • , Wanqiang Sheng
    • , Chuanyun Xu
    • , Hao Chen
    • , Jian Ouyang
    • , Siqing Wang
    • , Dominic Ling
    • , Pang-Hung Hsu
    • , Lee Zou
    • , Ashwini Jambhekar
    • , Chuan He
    •  & Yang Shi
    Nature 543, 573–576
  • Research |

    The C-to-U deamination at position 32 of tRNAThr in Trypanosoma brucei requires two enzymatic activities and proceeds via formation of a 3-methylcytosine intermediate, supporting the notion of a coupled modification system.

    • Mary Anne T. Rubio
    • , Kirk W. Gaston
    • , Katherine M. McKenney
    • , Ian M. C. Fleming
    • , Zdeněk Paris
    • , Patrick A. Limbach
    •  & Juan D. Alfonzo
    Nature 542, 494–497
  • Reviews |

    Although it has been known for decades that RNA is subjected to numerous covalent modifications, there has been a recent surge in interest driven by sequencing-based transcriptome-wide detection methods and the realization that RNA modifications have important roles in diverse biological processes. This Review describes the range of detection strategies for RNA modifications, their particular strengths and limitations, and how responsible and complementary application of these techniques will be required to ensure the quality and interpretability of the rapidly accumulating data sets.

    • Mark Helm
    •  & Yuri Motorin

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