Proteomic analysis

Proteomic analysis (proteomics) refers to the systematic identification and quantification of the complete complement of proteins (the proteome) of a biological system (cell, tissue, organ, biological fluid, or organism) at a specific point in time. Mass spectrometry is the technique most often used for proteomic analysis.

Latest Research and Reviews

  • Research | | open

    Jingshu Xu et al. did a comprehensive post-mortem proteomics analysis across six brain regions obtained from Alzheimer’s disease patients and controls. With this spatial proteomics approach, they identified new disease-related pathways and a gradient of protein expression changes that correlates with the pathology of the affected regions.

    • Jingshu Xu
    • , Stefano Patassini
    • , Nitin Rustogi
    • , Isabel Riba-Garcia
    • , Benjamin D. Hale
    • , Alexander M Phillips
    • , Henry Waldvogel
    • , Robert Haines
    • , Phil Bradbury
    • , Adam Stevens
    • , Richard L. M. Faull
    • , Andrew W. Dowsey
    • , Garth J. S. Cooper
    •  & Richard D. Unwin
  • Research | | open

    Spatial proteomics allows studying cellular protein localisations at system-wide scale. Here, the authors show that combining the previously developed hyperLOPIT method with differential centrifugation yields protein localisation maps at suborganellar resolution while reducing analysis time and input material.

    • Aikaterini Geladaki
    • , Nina Kočevar Britovšek
    • , Lisa M. Breckels
    • , Tom S. Smith
    • , Owen L. Vennard
    • , Claire M. Mulvey
    • , Oliver M. Crook
    • , Laurent Gatto
    •  & Kathryn S. Lilley
  • Research |

    Theralin, which simultaneously fixes and decalcifies bone tissue, was compared with formalin fixation with acid decalcification in primary bone cancer cases. Use of theralin improved (a) sample processing time, (b) tissue histomorphology, (c) protein and DNA extractability, and (d) enabled standard FISH staining in bone. This unlocks the molecular archive within bone for the standard tissue analysis pipeline.

    • Claudius Mueller
    • , Marco Gambarotti
    • , Stefania Benini
    • , Piero Picci
    • , Alberto Righi
    • , Monica Stevanin
    • , Sabine Hombach-Klonisch
    • , Dana Henderson
    • , Lance Liotta
    •  & Virginia Espina
  • Research | | open

    AMPK is regulates cellular energy and has been extensively studied, although our knowledge of downstream substrates is incomplete. Here, Chen et al. perform global quantitative analysis for AMPK-dependent sites and validate one hit, ARMC10, as a direct AMPK effector of mitochondrial dynamics.

    • Zhen Chen
    • , Caoqi Lei
    • , Chao Wang
    • , Nan Li
    • , Mrinal Srivastava
    • , Mengfan Tang
    • , Huimin Zhang
    • , Jong Min Choi
    • , Sung Yun Jung
    • , Jun Qin
    •  & Junjie Chen

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