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Protein enrichment is a technique by which proteins of a particular type in a biological sample are concentrated for further analysis and identification. Enrichment is used to help concentrate low-abundance proteins to improve their downstream analysis.
This protocol enables the study of protein complexes by trapping them in virus-like particles and thereby preserving cellular integrity. The Virotrap protocol is complemented by SFINX, an online data analysis tool for interactomics studies.
A platform for isolating low-abundance protein complexes from Arabidopsis seedlings and cell cultures is described. Its power resides in an improved TAP tag combined with ultrasensitive MS and filtering against a list of nonspecific proteins.