Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer).
Protein enrichment is a technique by which proteins of a particular type in a biological sample are concentrated for further analysis and identification. Enrichment is used to help concentrate low-abundance proteins to improve their downstream analysis.
This protocol enables the study of protein complexes by trapping them in virus-like particles and thereby preserving cellular integrity. The Virotrap protocol is complemented by SFINX, an online data analysis tool for interactomics studies.
A platform for isolating low-abundance protein complexes from Arabidopsis seedlings and cell cultures is described. Its power resides in an improved TAP tag combined with ultrasensitive MS and filtering against a list of nonspecific proteins.
Biochemical methods have typically been underused in Drosophila research owing to technical challenges. Here Depner et al. describe a simple CNS fractionation method that yields ∼4 mg of synaptic membrane protein per 1 g of adult fly heads.