Protein enrichment articles from across Nature Portfolio

The authors provide a detailed protocol for the isolation of four membrane protein complexes (transmembrane channel-like proteins 1 and 2, lipid transfer protein and ‘Protein S’) from transgenic C. elegans.

Lateral flow assays are valuable rapid diagnostic tests, but low sensitivity can hinder their precision. Here, the authors report an enrichment method using nanoporous AAO and red blood cell membranes, which when applied to patient samples prior to analysis can improve sensitivity up to 20-fold.

The newly transcribed RNA-bound proteome (or RNA interactome) is captured using this protocol by time-dependent incorporation of 5-ethynyluridine into newly transcribed RNAs followed by UV-mediated RNA-protein cross-linking, click-chemistry-aided biotinylation and affinity isolation of RNA–protein complexes.

The in vivo identification of proteins secreted from a specific cell type or tissue remains challenging. Here, the authors develop a proximity labeling-based method to selectively label secreted proteins and combine it with proteomics to identify liver secretory proteins in mouse plasma.

Swimming to a new way of decoding messages at the cell membrane.