Protein design

Protein design (or protein engineering) is a technique by which proteins with enhanced or novel functional properties are created. Proteins can be engineered by rational design, which typically uses computational tools to identify useful mutations, or by directed evolution, which uses random mutagenesis coupled with a selection process to identify desired variants.

Featured

Latest Research and Reviews

News and Comment

  • News and Views |

    A new method introduces ubiquitin or ubiquitin-like proteins at specific sites in any protein without the requirement of the cellular ubiquitylation machinery. This will help decipher the code by which these modifications control cellular processes.

    • Amit Kumar Singh Gautam
    •  & Andreas Matouschek
  • News and Views |

    Faster-than-transcription control of cellular activities is an important but challenging engineering target. Using split ferredoxins and induced dimerization or conformational changes, newly developed metalloprotein switches provide a fast method to control electron flux.

    • Michaela TerAvest
  • News and Views |

    Two protein circuit systems, split-protease-cleavable orthogonal coiled-coil logic (SPOC logic) and circuits of hacked orthogonal modular proteases (CHOMP), have been developed to permit rapid and logic function-based control of mammalian cellular signaling.

    • Yiqian Wu
    •  & Yingxiao Wang
  • Comments and Opinion |

    The rich repertoire of T cell receptors is awaiting large-scale profiling.

    • Nicole Rusk