Protein delivery

Definition

Protein delivery is the suite of methods by which proteins can be introduced into cells. Proteins can be exploited to produce potentially highly specific drugs. Protein delivery methods must maintain bioavailability and activity of proteins and should not be toxic.

Latest Research and Reviews

  • Research | | open

    • Valérie Deckert
    • , Stéphanie Lemaire
    • , Pierre-Jean Ripoll
    • , Jean-Paul Pais Barros
    • , Jérôme Labbé
    • , Caroline Chabert-Le Borgne
    • , Véronique Turquois
    • , Guillaume Maquart
    • , Delphine Larose
    • , Nicolas Desroche
    • , Franck Ménétrier
    • , Naig Guern
    • , Lorène J. Lebrun
    • , Catherine Desrumaux
    • , Thomas Gautier
    • , Jacques Grober
    • , Charles Thomas
    • , David Masson
    • , Louis-Marie Houdebine
    •  & Laurent Lagrost
  • Research | | open

    Third-generation base editors consist of a catalytically disabled Cas9 fused to a cytidine deaminase and a base excision repair inhibitor, enabling efficient, precise editing of individual base pairs in DNA. Here the authors describe engineering and protein delivery of base editors to improve their DNA specificity and enable specific base editing in live animals.

    • Holly A. Rees
    • , Alexis C. Komor
    • , Wei-Hsi Yeh
    • , Joana Caetano-Lopes
    • , Matthew Warman
    • , Albert S. B. Edge
    •  & David R. Liu
  • Research | | open

    The depletion of antigen-specific, deleterious antibodies during therapy and diagnosis remains an unsolved challenge. Here the authors develop ‘Seldegs’, antigens linked to human Fc fragments with high affinity for the neonatal Fc receptor FcRn, to deplete MOG- and HER2-specific antibodies in mice.

    • Siva Charan Devanaboyina
    • , Priyanka Khare
    • , Dilip K. Challa
    • , Raimund J. Ober
    •  & E. Sally Ward
  • Research |

    The trapping of antibodies in endosomes often limits their use for intracellular targeting. Now, a single amino acid substitution on a spider-venom peptide has been shown to attenuate the cell membrane lytic activity and enables the selective rupturing of endosomal membranes. The peptide can be used to facilitate the escape of antibodies from endosomes into the cytosol.

    • Misao Akishiba
    • , Toshihide Takeuchi
    • , Yoshimasa Kawaguchi
    • , Kentarou Sakamoto
    • , Hao-Hsin Yu
    • , Ikuhiko Nakase
    • , Tomoka Takatani-Nakase
    • , Fatemeh Madani
    • , Astrid Gräslund
    •  & Shiroh Futaki
  • Research | | open

    The type III secretion system is a needle-like molecular machine under tight regulatory control. Here the authors construct a synthetic type III secretion system gene cluster by deconstructing and rebuilding the wild-type Salmonella pathogenicity island 1.

    • Miryoung Song
    • , David J. Sukovich
    • , Luciano Ciccarelli
    • , Julia Mayr
    • , Jesus Fernandez-Rodriguez
    • , Ethan A. Mirsky
    • , Alex C. Tucker
    • , D. Benjamin Gordon
    • , Thomas C. Marlovits
    •  & Christopher A. Voigt

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