Perturbations - Latest research and news

Latest Research and Reviews

Pushed to extremes: distinct effects of high temperature versus pressure on the structure of STEP

A multi-perturbation crystallographic study on the atypical phosphatase STEP reveals that temperature and pressure are powerful macromolecular perturbations, with distinct effects on protein backbone, side-chain conformations, and ordered solvent.
- Liliana Guerrero
- Ali Ebrahim
- Daniel A. Keedy
ResearchOpen Access12 Jan 2024 Communications Biology
Volume: 7, P: 59
Fast functional mapping of ligand-gated ion channels

A multi-channel solution exchange system supplies up to 30 different external solutions to ligand-gated ion channels in a membrane patch in rapid succession, allowing to determine detailed dose-response relationships and dynamics in seconds.
- Ralf Schmauder
- Thomas Eick
- Klaus Benndorf
ResearchOpen Access02 Oct 2023 Communications Biology
Volume: 6, P: 1003
Retrospective study for the universal applicability of the residue-based linear free energy relationship in the two-state exchange of protein molecules
- Daisuke Fujinami
- Seiichiro Hayashi
- Daisuke Kohda
ResearchOpen Access07 Oct 2022 Scientific Reports
Volume: 12, P: 16843
Halogenation of tyrosine perturbs large-scale protein self-organization

Sun et al. has studied the genetically encodable halotyrosines in proteins of the prokaryotic cell division machinery to elucidate the general role of halogenation in cellular lifespan and oxidative damage-induced diseases such as aging and cancer.
- Huan Sun
- Haiyang Jia
- Nediljko Budisa
ResearchOpen Access17 Aug 2022 Nature Communications
Volume: 13, P: 4843
Voltage-gated proton channels from fungi highlight role of peripheral regions in channel activation

Chang Zhao and Francesco Tombola identify and characterize distinct biophysical properties among voltage-gated proton channels in Basidiomycota and Ascomycota fungi. By generating chimeric channel proteins, the authors also report that intra- and extracellular peripheral regions are key determinants of channel properties, providing important insight on fungal physiology.
- Chang Zhao
- Francesco Tombola
ResearchOpen Access26 Feb 2021 Communications Biology
Volume: 4, P: 261
Sensing the allosteric force

Almost all allosteric systems are large multi-domain proteins which complicates the investigation of the mechanism in atomistic detail. Here authors designed a small allosteric protein system using the photocontrollable PDZ3 domain, that allows controlling allostery between the binding and allosteric site in both directions.
- Olga Bozovic
- Brankica Jankovic
- Peter Hamm
ResearchOpen Access17 Nov 2020 Nature Communications
Volume: 11, P: 5841