PCR-based techniques

Definition

PCR-based techniques are methods that rely on the polymerase chain reaction (PCR) to amplify stretches of DNA by creating many identical or near-identical copies. For example, PCR amplification can be used to isolate the sequence at a specific location in the genome (genotyping).

Latest Research and Reviews

News and Comment

  • News |

    PCR duplicates—sequencing reads from the same original genomic fragment—can cause headaches. But there are remedies.

    • Vivien Marx
    Nature Methods 14, 473–476
  • News |

    Infidelity is painful in life and in the lab. The former is better left to other publications; the latter is best not ignored, especially in the context of PCR-based DNA amplification.

    • Vivien Marx
    Nature Methods 13, 475–479
  • Comments and Opinion |

    Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

    • Stephen A Bustin
    • , Vladimir Benes
    • , Jeremy Garson
    • , Jan Hellemans
    • , Jim Huggett
    • , Mikael Kubista
    • , Reinhold Mueller
    • , Tania Nolan
    • , Michael W Pfaffl
    • , Gregory Shipley
    • , Carl T Wittwer
    • , Peter Schjerling
    • , Philip J Day
    • , Mónica Abreu
    • , Begoña Aguado
    • , Jean-François Beaulieu
    • , Anneleen Beckers
    • , Sara Bogaert
    • , John A Browne
    • , Fernando Carrasco-Ramiro
    • , Liesbeth Ceelen
    • , Kate Ciborowski
    • , Pieter Cornillie
    • , Stephanie Coulon
    • , Ann Cuypers
    • , Sara De Brouwer
    • , Leentje De Ceuninck
    • , Jurgen De Craene
    • , Hélène De Naeyer
    • , Ward De Spiegelaere
    • , Kato Deckers
    • , Annelies Dheedene
    • , Kaat Durinck
    • , Margarida Ferreira-Teixeira
    • , Annelies Fieuw
    • , Jack M Gallup
    • , Sandra Gonzalo-Flores
    • , Karen Goossens
    • , Femke Heindryckx
    • , Elizabeth Herring
    • , Hans Hoenicka
    • , Laura Icardi
    • , Rolf Jaggi
    • , Farzad Javad
    • , Michael Karampelias
    • , Frederick Kibenge
    • , Molly Kibenge
    • , Candy Kumps
    • , Irina Lambertz
    • , Tim Lammens
    • , Amelia Markey
    • , Peter Messiaen
    • , Evelien Mets
    • , Sofia Morais
    • , Alberto Mudarra-Rubio
    • , Justine Nakiwala
    • , Hilde Nelis
    • , Pal A Olsvik
    • , Claudina Pérez-Novo
    • , Michelle Plusquin
    • , Tony Remans
    • , Ali Rihani
    • , Paulo Rodrigues-Santos
    • , Pieter Rondou
    • , Rebecca Sanders
    • , Katharina Schmidt-Bleek
    • , Kerstin Skovgaard
    • , Karen Smeets
    • , Laura Tabera
    • , Stefan Toegel
    • , Tim Van Acker
    • , Wim Van den Broeck
    • , Joni Van der Meulen
    • , Mireille Van Gele
    • , Gert Van Peer
    • , Mario Van Poucke
    • , Nadine Van Roy
    • , Sarah Vergult
    • , Joris Wauman
    • , Marina Tshuikina-Wiklander
    • , Erik Willems
    • , Sara Zaccara
    • , Fjoralba Zeka
    •  & Jo Vandesompele
    Nature Methods 10, 1063–1067