Oligo delivery articles within Nature Communications

Featured

• Article
  25 February 2023 | Open Access

  Single-cell quantification and dose-response of cytosolic siRNA delivery

  Endosomal escape and subsequent cytosolic delivery of siRNA therapeutics is inefficient, and quantification is difficult. Here the authors report a confocal microscopy-based method to quantify cytosolic delivery of fluorescently labelled siRNA during lipid-mediated delivery.

  • Hampus Hedlund
  • , Hampus Du Rietz
  •  & Anders Wittrup

• Article
  14 April 2020 | Open Access

  Imaging small molecule-induced endosomal escape of siRNA

  Therapeutic siRNA becomes trapped in endosomes, limiting its efficacy. Here the authors use fluorescently-tagged galectin-9 as a biosensor for membrane damage to monitor endosomal escape of cholesterol-conjugated siRNA following treatment of small molecule membrane-destabilising drugs.

  • Hampus Du Rietz
  • , Hampus Hedlund
  •  & Anders Wittrup

• Article
  20 February 2020 | Open Access

  Naturally-occurring cholesterol analogues in lipid nanoparticles induce polymorphic shape and enhance intracellular delivery of mRNA

  Endosomal sequestration of lipid-based nanoparticles is a barrier to delivery of nucleic acids. Here the authors test an array of cholesterol variants and perform in-depth investigation of nanoparticle shape, internal structure and intracellular trafficking.

  • Siddharth Patel
  • , N. Ashwanikumar
  •  & Gaurav Sahay

• Article
  24 April 2019 | Open Access

  In vivo rendezvous of small nucleic acid drugs with charge-matched block catiomers to target cancers

  Nanoparticle delivery of siRNA has problems with penetration and off target accumulation. Here, the authors report on the development of Y-shaped block catiomers which dynamically wrap around siRNA; demonstrate increased circulation times and delivery into hard to reach brain and pancreas tumour models.

  • Sumiyo Watanabe
  • , Kotaro Hayashi
  •  & Kazunori Kataoka

• Article
  11 June 2018 | Open Access

  Modular cell-internalizing aptamer nanostructure enables targeted delivery of large functional RNAs in cancer cell lines

  Large RNAs and ribonucleoprotein complexes have shown potential as novel therapeutic agents, but their targeted delivery to cells is still challenging. Here the authors present a modular aptamer nanostructure for intracellular delivery of RNAs up to 250 nucleotides to cancer cells.

  • David Porciani
  • , Leah N. Cardwell
  •  & Donald H. Burke

• Article
  24 November 2016 | Open Access

  A DNA dual lock-and-key strategy for cell-subtype-specific siRNA delivery

  Delivery of siRNA to target cells is essential for in vivogene therapy. Here the authors demonstrate an oligonucleotide aptamer that targets therapeutic siRNA to a specific cell type.

  • Kewei Ren
  • , Ying Liu
  •  & Huangxian Ju