Nucleolus

Definition

The nucleolus is a region within the nucleus that primarily functions as the site for the transcription of ribosomal RNA (rRNA) genes, the processing of precursor rRNAs into mature components of ribosomes, and the initial steps of ribosome assembly.

Latest Research and Reviews

  • Reviews |

    In addition to membrane-bound organelles, eukaryotic cells feature various membraneless compartments, including the centrosome, the nucleolus and various granules. Many of these compartments form through liquid–liquid phase separation, and the principles, mechanisms and regulation of their assembly as well as their cellular functions are now beginning to emerge.

    • Salman F. Banani
    • , Hyun O. Lee
    • , Anthony A. Hyman
    •  & Michael K. Rosen
  • Research | | open

    Nucleoplasmic translocation of NPM1 is integral to nucleolar stress sensing. Here, the authors show that nucleolar oxidation is a general cellular stress response, and that oxidation-related glutathionylation of NPM1 triggers its translocation and facilitates p53 activation.

    • Kai Yang
    • , Ming Wang
    • , Yuzheng Zhao
    • , Xuxu Sun
    • , Yi Yang
    • , Xie Li
    • , Aiwu Zhou
    • , Huilin Chu
    • , Hu Zhou
    • , Jianrong Xu
    • , Mian Wu
    • , Jie Yang
    •  & Jing Yi
  • Research | | open

    The nucleolus is a specialized functional domain of the nucleus where ribosome biogenesis is initiated and also implicated in a p53-dependent anti-tumor surveillance. Here the authors use a quantitative imaging approach to detail the role of each ribosomal protein on the structural integrity of the nucleolus and p53 homeostasis.

    • Emilien Nicolas
    • , Pascaline Parisot
    • , Celina Pinto-Monteiro
    • , Roxane de Walque
    • , Christophe De Vleeschouwer
    •  & Denis L. J. Lafontaine
  • Research |

    DNA damage induces silencing of ribosomal RNA (rRNA) transcription. Stucki and colleagues reveal that rRNA silencing is an ATM-dependent pan-nuclear response to irradiation, in which the nucleolar protein Treacle targets DNA-damage protein NBS1 to nucleoli.

    • Dorthe H. Larsen
    • , Flurina Hari
    • , Julie A. Clapperton
    • , Myriam Gwerder
    • , Katrin Gutsche
    • , Matthias Altmeyer
    • , Stephanie Jungmichel
    • , Luis I. Toledo
    • , Daniel Fink
    • , Maj-Britt Rask
    • , Merete Grøfte
    • , Claudia Lukas
    • , Michael L. Nielsen
    • , Stephen J. Smerdon
    • , Jiri Lukas
    •  & Manuel Stucki
    Nature Cell Biology 16, 792–803
  • Research |

    Structurally polymorphic C9orf72 hexanucleotide repeats cause an impairment in transcriptional processivity and lead to accumulation of truncated repeat-containing transcripts that bind to specific ribonucleoproteins, such as nucleolin, in a conformation-dependent manner resulting in nucleolar stress and C9orf72-linked pathology in amyotrophic lateral sclerosis and frontotemporal dementia.

    • Aaron R. Haeusler
    • , Christopher J. Donnelly
    • , Goran Periz
    • , Eric A. J. Simko
    • , Patrick G. Shaw
    • , Min-Sik Kim
    • , Nicholas J. Maragakis
    • , Juan C. Troncoso
    • , Akhilesh Pandey
    • , Rita Sattler
    • , Jeffrey D. Rothstein
    •  & Jiou Wang
    Nature 507, 195–200