Nanocrystallography

Nanocrystallography (serial femtosecond crystallography) is a protein X-ray crystallography technique that uses femtosecond pulses from an X-ray free-electron laser to collect diffraction snapshots from a stream of single nano-sized crystals of a macromolecule, in order to solve a high-resolution three-dimensional macromolecular structure.

Latest Research and Reviews

  • Research |

    Crystallography and spectroscopy are used to solve high-resolution structures of the intermediates of Kok’s S-state clock in photosystem II.

    • Jan Kern
    • , Ruchira Chatterjee
    • , Iris D. Young
    • , Franklin D. Fuller
    • , Louise Lassalle
    • , Mohamed Ibrahim
    • , Sheraz Gul
    • , Thomas Fransson
    • , Aaron S. Brewster
    • , Roberto Alonso-Mori
    • , Rana Hussein
    • , Miao Zhang
    • , Lacey Douthit
    • , Casper de Lichtenberg
    • , Mun Hon Cheah
    • , Dmitry Shevela
    • , Julia Wersig
    • , Ina Seuffert
    • , Dimosthenis Sokaras
    • , Ernest Pastor
    • , Clemens Weninger
    • , Thomas Kroll
    • , Raymond G. Sierra
    • , Pierre Aller
    • , Agata Butryn
    • , Allen M. Orville
    • , Mengning Liang
    • , Alexander Batyuk
    • , Jason E. Koglin
    • , Sergio Carbajo
    • , Sébastien Boutet
    • , Nigel W. Moriarty
    • , James M. Holton
    • , Holger Dobbek
    • , Paul D. Adams
    • , Uwe Bergmann
    • , Nicholas K. Sauter
    • , Athina Zouni
    • , Johannes Messinger
    • , Junko Yano
    •  & Vittal K. Yachandra
    Nature 563, 421-425
  • Research | | open

    The new European X-Ray Free-Electron Laser (EuXFEL) is the first XFEL that generates X-ray pulses with a megahertz inter-pulse spacing. Here the authors demonstrate that high-quality and damage-free protein structures can be obtained with the currently available 1.1 MHz repetition rate pulses using lysozyme as a test case and furthermore present a β-lactamase structure.

    • Max O. Wiedorn
    • , Dominik Oberthür
    • , Richard Bean
    • , Robin Schubert
    • , Nadine Werner
    • , Brian Abbey
    • , Martin Aepfelbacher
    • , Luigi Adriano
    • , Aschkan Allahgholi
    • , Nasser Al-Qudami
    • , Jakob Andreasson
    • , Steve Aplin
    • , Salah Awel
    • , Kartik Ayyer
    • , Saša Bajt
    • , Imrich Barák
    • , Sadia Bari
    • , Johan Bielecki
    • , Sabine Botha
    • , Djelloul Boukhelef
    • , Wolfgang Brehm
    • , Sandor Brockhauser
    • , Igor Cheviakov
    • , Matthew A. Coleman
    • , Francisco Cruz-Mazo
    • , Cyril Danilevski
    • , Connie Darmanin
    • , R. Bruce Doak
    • , Martin Domaracky
    • , Katerina Dörner
    • , Yang Du
    • , Hans Fangohr
    • , Holger Fleckenstein
    • , Matthias Frank
    • , Petra Fromme
    • , Alfonso M. Gañán-Calvo
    • , Yaroslav Gevorkov
    • , Klaus Giewekemeyer
    • , Helen Mary Ginn
    • , Heinz Graafsma
    • , Rita Graceffa
    • , Dominic Greiffenberg
    • , Lars Gumprecht
    • , Peter Göttlicher
    • , Janos Hajdu
    • , Steffen Hauf
    • , Michael Heymann
    • , Susannah Holmes
    • , Daniel A. Horke
    • , Mark S. Hunter
    • , Siegfried Imlau
    • , Alexander Kaukher
    • , Yoonhee Kim
    • , Alexander Klyuev
    • , Juraj Knoška
    • , Bostjan Kobe
    • , Manuela Kuhn
    • , Christopher Kupitz
    • , Jochen Küpper
    • , Janine Mia Lahey-Rudolph
    • , Torsten Laurus
    • , Karoline Le Cong
    • , Romain Letrun
    • , P. Lourdu Xavier
    • , Luis Maia
    • , Filipe R. N. C. Maia
    • , Valerio Mariani
    • , Marc Messerschmidt
    • , Markus Metz
    • , Davide Mezza
    • , Thomas Michelat
    • , Grant Mills
    • , Diana C. F. Monteiro
    • , Andrew Morgan
    • , Kerstin Mühlig
    • , Anna Munke
    • , Astrid Münnich
    • , Julia Nette
    • , Keith A. Nugent
    • , Theresa Nuguid
    • , Allen M. Orville
    • , Suraj Pandey
    • , Gisel Pena
    • , Pablo Villanueva-Perez
    • , Jennifer Poehlsen
    • , Gianpietro Previtali
    • , Lars Redecke
    • , Winnie Maria Riekehr
    • , Holger Rohde
    • , Adam Round
    • , Tatiana Safenreiter
    • , Iosifina Sarrou
    • , Tokushi Sato
    • , Marius Schmidt
    • , Bernd Schmitt
    • , Robert Schönherr
    • , Joachim Schulz
    • , Jonas A. Sellberg
    • , M. Marvin Seibert
    • , Carolin Seuring
    • , Megan L. Shelby
    • , Robert L. Shoeman
    • , Marcin Sikorski
    • , Alessandro Silenzi
    • , Claudiu A. Stan
    • , Xintian Shi
    • , Stephan Stern
    • , Jola Sztuk-Dambietz
    • , Janusz Szuba
    • , Aleksandra Tolstikova
    • , Martin Trebbin
    • , Ulrich Trunk
    • , Patrik Vagovic
    • , Thomas Ve
    • , Britta Weinhausen
    • , Thomas A. White
    • , Krzysztof Wrona
    • , Chen Xu
    • , Oleksandr Yefanov
    • , Nadia Zatsepin
    • , Jiaguo Zhang
    • , Markus Perbandt
    • , Adrian P. Mancuso
    • , Christian Betzel
    • , Henry Chapman
    •  & Anton Barty
  • Research | | open

    The European X-ray free-electron laser (EuXFEL) in Hamburg is the first megahertz (MHz) repetition rate XFEL. Here the authors use lysozyme crystals and microcrystals from jack bean proteins and demonstrate that damage-free high quality data can be collected at a MHz repetition rate.

    • Marie Luise Grünbein
    • , Johan Bielecki
    • , Alexander Gorel
    • , Miriam Stricker
    • , Richard Bean
    • , Marco Cammarata
    • , Katerina Dörner
    • , Lars Fröhlich
    • , Elisabeth Hartmann
    • , Steffen Hauf
    • , Mario Hilpert
    • , Yoonhee Kim
    • , Marco Kloos
    • , Romain Letrun
    • , Marc Messerschmidt
    • , Grant Mills
    • , Gabriela Nass Kovacs
    • , Marco Ramilli
    • , Christopher M. Roome
    • , Tokushi Sato
    • , Matthias Scholz
    • , Michel Sliwa
    • , Jolanta Sztuk-Dambietz
    • , Martin Weik
    • , Britta Weinhausen
    • , Nasser Al-Qudami
    • , Djelloul Boukhelef
    • , Sandor Brockhauser
    • , Wajid Ehsan
    • , Moritz Emons
    • , Sergey Esenov
    • , Hans Fangohr
    • , Alexander Kaukher
    • , Thomas Kluyver
    • , Max Lederer
    • , Luis Maia
    • , Maurizio Manetti
    • , Thomas Michelat
    • , Astrid Münnich
    • , Florent Pallas
    • , Guido Palmer
    • , Gianpietro Previtali
    • , Natascha Raab
    • , Alessandro Silenzi
    • , Janusz Szuba
    • , Sandhya Venkatesan
    • , Krzysztof Wrona
    • , Jun Zhu
    • , R. Bruce Doak
    • , Robert L. Shoeman
    • , Lutz Foucar
    • , Jacques-Philippe Colletier
    • , Adrian P. Mancuso
    • , Thomas R. M. Barends
    • , Claudiu A. Stan
    •  & Ilme Schlichting
  • Research | | open

    The transient receptor potential channel TRPV6 mediates calcium uptake in epithelia and its expression is increased in several cancer types. Here, authors present structures of TRPV6 bound to 2-APB, a TRPV6 inhibitor, and show that 2-APB induces TRPV6 channel closure by modulating protein–lipid interactions.

    • Appu K. Singh
    • , Kei Saotome
    • , Luke L. McGoldrick
    •  & Alexander I. Sobolevsky

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