Mutagenesis

Definition

Mutagenesis is the process of generating a genetic mutation. This may occur spontaneously or be induced by mutagens. Researchers also use a number of techniques to create mutations, including transposon mutagenesis to generate random gene knockouts, and site-directed mutagenesis, which utilises the polymerase chain reaction to introduce specific mutations.

Latest Research and Reviews

  • Research | | open

    The full extent of the genetic basis for hearing impairment is unknown. Here, as part of the International Mouse Phenotyping Consortium, the authors perform a hearing loss screen in 3006 mouse knockout strains and identify 52 new candidate genes for genetic hearing loss.

    • Michael R. Bowl
    • , Michelle M. Simon
    • , Neil J. Ingham
    • , Simon Greenaway
    • , Luis Santos
    • , Heather Cater
    • , Sarah Taylor
    • , Jeremy Mason
    • , Natalja Kurbatova
    • , Selina Pearson
    • , Lynette R. Bower
    • , Dave A. Clary
    • , Hamid Meziane
    • , Patrick Reilly
    • , Osamu Minowa
    • , Lois Kelsey
    • , Glauco P. Tocchini-Valentini
    • , Xiang Gao
    • , Allan Bradley
    • , William C. Skarnes
    • , Mark Moore
    • , Arthur L. Beaudet
    • , Monica J. Justice
    • , John Seavitt
    • , Mary E. Dickinson
    • , Wolfgang Wurst
    • , Martin Hrabe de Angelis
    • , Yann Herault
    • , Shigeharu Wakana
    • , Lauryl M. J. Nutter
    • , Ann M. Flenniken
    • , Colin McKerlie
    • , Stephen A. Murray
    • , Karen L. Svenson
    • , Robert E. Braun
    • , David B. West
    • , K. C. Kent Lloyd
    • , David J. Adams
    • , Jacqui White
    • , Natasha Karp
    • , Paul Flicek
    • , Damian Smedley
    • , Terrence F. Meehan
    • , Helen E. Parkinson
    • , Lydia M. Teboul
    • , Sara Wells
    • , Karen P. Steel
    • , Ann-Marie Mallon
    •  & Steve D. M. Brown
  • Research | | open

    High-throughput genetic screens in animals could benefit from an easy way to mark positive hits. Here the authors introduce photo-highlighting using a photoconvertible fluorescent protein, and in combination with stimulated Raman scattering (SRS) microscopy, define a role for BMP signaling in lipid metabolism in C. elegans.

    • Yong Yu
    • , Ayse Sena Mutlu
    • , Harrison Liu
    •  & Meng C. Wang
  • Protocols |

    Knockout Sudoku allows construction of whole-genome knockout collections for a wide range of microorganisms at a lower cost and increased speed, using combinatorial pooling, next-generation sequencing, and a Bayesian inference algorithm to process and annotate extremely large progenitor transposon insertion mutant collections.

    • Isao A Anzai
    • , Lev Shaket
    • , Oluwakemi Adesina
    • , Michael Baym
    •  & Buz Barstow
    Nature Protocols 12, 2110–2137
  • Research |

    The authors describe a two-cell-type CRISPR screen to identify tumour-intrinsic genes that regulate the sensitivity of cancer cells to effector T cell function.

    • Shashank J. Patel
    • , Neville E. Sanjana
    • , Rigel J. Kishton
    • , Arash Eidizadeh
    • , Suman K. Vodnala
    • , Maggie Cam
    • , Jared J. Gartner
    • , Li Jia
    • , Seth M. Steinberg
    • , Tori N. Yamamoto
    • , Anand S. Merchant
    • , Gautam U. Mehta
    • , Anna Chichura
    • , Ophir Shalem
    • , Eric Tran
    • , Robert Eil
    • , Madhusudhanan Sukumar
    • , Eva Perez Guijarro
    • , Chi-Ping Day
    • , Paul Robbins
    • , Steve Feldman
    • , Glenn Merlino
    • , Feng Zhang
    •  & Nicholas P. Restifo
    Nature 548, 537–542

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