The house mouse, Mus musculus, is a small rodent and a classic vertebrate model organism for basic and biomedical research. It is attractive due to the existence of many well-characterized strains, the ability to make transgenics and to perform gene targeting by homologous recombination.

Latest Research and Reviews

  • Protocols |

    In this protocol, the authors present an experimental metastasis assay in which cancer cells are injected into the tail vein of a mouse, and the resulting secondary organ colonization is assessed, primarily in the lung, 10 d later.

    • Anneliese O Speak
    • , Agnieszka Swiatkowska
    • , Natasha A Karp
    • , Mark J Arends
    • , David J Adams
    •  & Louise van der Weyden
    Nature Protocols 12, 2465–2477
  • Research |

    Characterizing the essentiality of human genes provides insights into gene function and genome evolution and facilitates the clinical interpretation of genetic variants. This article analyses essentiality metrics based on the statistical intolerance to loss-of-function mutations in human population sequencing studies and discusses commonalities and distinctions relative to data sets from knockout mice and functional genomics screens in human cell culture. Implications for disease genetics and extrapolation to non-coding regions are also discussed.

    • István Bartha
    • , Julia di Iulio
    • , J. Craig Venter
    •  & Amalio Telenti
  • Protocols |

    In this protocol, the skull overlying the cerebellum is removed and a window is applied, enabling intravital imaging to provide a detailed characterization of dynamic processes in this region of the mouse brain.

    • Vasileios Askoxylakis
    • , Mark Badeaux
    • , Sylvie Roberge
    • , Ana Batista
    • , Ned Kirkpatrick
    • , Matija Snuderl
    • , Zohreh Amoozgar
    • , Giorgio Seano
    • , Gino B Ferraro
    • , Sampurna Chatterjee
    • , Lei Xu
    • , Dai Fukumura
    • , Dan G Duda
    •  & Rakesh K Jain
    Nature Protocols 12, 2251–2262

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