Model vertebrates articles from across Nature Portfolio

In this study, the authors show that a single dose of a combination of two human-like monoclonal antibodies protect mice in therapeutic treatment against challenges with ectromelia virus and monkeypox virus. Combination treatment provided more effective viral clearance than single antibody treatment.

Generation of an endogenous Cyp1a1 luciferase reporter mouse model to dynamically and longitudinally visualize AHR activity throughout lifespan and in response to ligand exposure, reveals prolonged AHR activation in the lung.

Co-infections are much less studied than single pathogen infections. Here, the authors show that co-infection with two unrelated viruses, neurotropic Semliki Forest virus and influenza A virus, exacerbates influenza-related lung pathology and prolongs lung virus replication in a mouse model.

RoboEM enables automated proofreading of electron microscopy datasets using a strategy akin to that of self-steering cars. This decreases the need for manual proofreading of segmented datasets and facilitates connectomic analyses.

Improved green cAMP and red calcium sensors were developed to facilitate dual-color imaging in vivo. These sensors will allow studying the relationship between calcium and cAMP signaling.

Facemap tracks keypoints on the mouse face and feeds the information into a deep neural network to predict neural activity.

The requirement for a protospacer adjacent motif (PAM) is a well-known limitation of the CRISPR–Cas9 system, as it restricts the range of sequences that can be targeted. To address this limitation, we demonstrate a phage-assisted evolution approach for engineering a compact SlugCas9, simplifying its PAM requirement and broadening its DNA targeting scope.

Grid cells develop in rats soon after they leave the nest. Here, Ulsaker-Janke et al. show that preventing exposure to straight boundaries from birth delays, but does not prevent, grid cell maturation in adult rats.

In this Tools of the Trade article, Sarah Paramore (from the Devenport and Nelson labs) discusses the use of mouse strains carrying genomic alterations in PCP genes and how they can increase our understanding of mammalian planar cell polarity.