Methylation analysis

Methylation analysis is the study of chromosomal patterns of DNA or histone modification by methyl groups. The cytosine (C) base in DNA and lysine residue in histone tails can be methylated. Methylation constitutes an epigenetic mark because its location can affect the regulation of gene expression in a heritable fashion.

Latest Research and Reviews

  • Research | | open

    Our knowledge of DNA methylation systems in prokaryotes is mostly limited to those of culturable microbes. Here, Hiraoka et al. analyse DNA methylation patterns in metagenomic data from a microbial community, revealing new methylated motifs and experimentally validating the methyltransferases’ specificities.

    • Satoshi Hiraoka
    • , Yusuke Okazaki
    • , Mizue Anda
    • , Atsushi Toyoda
    • , Shin-ichi Nakano
    •  & Wataru Iwasaki
  • Protocols |

    In addition to canonical nucleotides, DNA contains various modified bases that contribute to the developmental and disease state of the organism. UHPLC–MS/MS with isotopically labeled standards is used in this protocol to quantify modified bases.

    • Franziska R. Traube
    • , Sarah Schiffers
    • , Katharina Iwan
    • , Stefanie Kellner
    • , Fabio Spada
    • , Markus Müller
    •  & Thomas Carell
    Nature Protocols 14, 283-312
  • Reviews |

    Disruptions to the epigenome occur during the pathogenesis of various human diseases. In this Review, Berdasco and Esteller describe the challenges and progress of harnessing epigenetic changes for clinical application, including for diagnostics, disease classification and therapeutics. They discuss applications to diverse diseases, such as cancer, neurological disease, immune disorders and viral infections.

    • María Berdasco
    •  & Manel Esteller
  • Research |

    An immunoprecipitation-based protocol is developed to analyse DNA methylation in small quantities of circulating cell-free DNA, and can detect and classify cancers in plasma samples from several tumour types.

    • Shu Yi Shen
    • , Rajat Singhania
    • , Gordon Fehringer
    • , Ankur Chakravarthy
    • , Michael H. A. Roehrl
    • , Dianne Chadwick
    • , Philip C. Zuzarte
    • , Ayelet Borgida
    • , Ting Ting Wang
    • , Tiantian Li
    • , Olena Kis
    • , Zhen Zhao
    • , Anna Spreafico
    • , Tiago da Silva Medina
    • , Yadon Wang
    • , David Roulois
    • , Ilias Ettayebi
    • , Zhuo Chen
    • , Signy Chow
    • , Tracy Murphy
    • , Andrea Arruda
    • , Grainne M. O’Kane
    • , Jessica Liu
    • , Mark Mansour
    • , John D. McPherson
    • , Catherine O’Brien
    • , Natasha Leighl
    • , Philippe L. Bedard
    • , Neil Fleshner
    • , Geoffrey Liu
    • , Mark D. Minden
    • , Steven Gallinger
    • , Anna Goldenberg
    • , Trevor J. Pugh
    • , Michael M. Hoffman
    • , Scott V. Bratman
    • , Rayjean J. Hung
    •  & Daniel D. De Carvalho
    Nature 563, 579-583

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