Methylases

Definition

Methylases are transfer enzymes that catalyze transfer of a methyl group (CH3 or Me) – termed methylation – from a donor molecule to an acceptor molecule.

Latest Research and Reviews

  • Research |

    Genome-wide localization and activity analysis of the de novo DNA methyltransferases DNMT3A and DNMT3B in mouse embryonic stem cells identifies overlapping and individual targeting preferences to the genome, including a role for DNMT3B in gene body methylation.

    • Tuncay Baubec
    • , Daniele F. Colombo
    • , Christiane Wirbelauer
    • , Juliane Schmidt
    • , Lukas Burger
    • , Arnaud R. Krebs
    • , Altuna Akalin
    •  & Dirk Schübeler
    Nature 520, 243–247
  • Research |

    Various biological processes are entrained by the day–night cycle to occur at a specific time of day. One way the circadian system exerts these effects is through post-transcriptional regulation. These authors show that a protein that transfers methyl groups onto several spliceosome subunits, PRMT5, is regulated by the light–dark cycle. Methylation of these subunits affects alternative splicing of some genes, thus making them subject to circadian control.

    • Sabrina E. Sanchez
    • , Ezequiel Petrillo
    • , Esteban J. Beckwith
    • , Xu Zhang
    • , Matias L. Rugnone
    • , C. Esteban Hernando
    • , Juan C. Cuevas
    • , Micaela A. Godoy Herz
    • , Ana Depetris-Chauvin
    • , Craig G. Simpson
    • , John W. S. Brown
    • , Pablo D. Cerdán
    • , Justin O. Borevitz
    • , Paloma Mas
    • , M. Fernanda Ceriani
    • , Alberto R. Kornblihtt
    •  & Marcelo J. Yanovsky
    Nature 468, 112–116
  • Research |

    A conserved protein from enteropathogenic Escherichia coli, NleE, inhibits innate immune defence against infection by disrupting the NF-κB signalling pathway through methylation of ubiquitin-chain sensing proteins.

    • Li Zhang
    • , Xiaojun Ding
    • , Jixin Cui
    • , Hao Xu
    • , Jing Chen
    • , Yi-Nan Gong
    • , Liyan Hu
    • , Yan Zhou
    • , Jianning Ge
    • , Qiuhe Lu
    • , Liping Liu
    • , She Chen
    •  & Feng Shao
    Nature 481, 204–208
  • Research |

    α-N-methylation is an unusual post-translational modification in which the amino-terminal residues of proteins are methylated. One example is the Ran guanine nucleotide-exchange factor, RCC1, which requires methylation for its association with chromatin. These authors describe the first α-N-methyltransferase, named N-terminal RCC1 methyltransferase (NRMT). They identify the NRMT recognition sequence and several new methylation targets, and demonstrate the importance of α-N-methylation for normal bipolar spindle formation and chromosome segregation.

    • Christine E. Schaner Tooley
    • , Janusz J. Petkowski
    • , Tara L. Muratore-Schroeder
    • , Jeremy L. Balsbaugh
    • , Jeffrey Shabanowitz
    • , Michal Sabat
    • , Wladek Minor
    • , Donald F. Hunt
    •  & Ian G. Macara
    Nature 466, 1125–1128

News and Comment