Liquid chromatography articles from across Nature Portfolio

This protocol details a method for profiling the glycosylation status of antigen-specific antibodies. The protocol consists of an antibody-capture step using an immunosorbent assay similar to enzyme-linked immunosorbent assay, followed by the characterization of captured antibodies using liquid chromatography–mass spectrometry.

The microbial metabolite, d-alanine, cannot be endogenously synthesized by mice, and is instead derived from the food and gut microbiota, with gut-absorbed d-alanine accumulating in the mouse pancreas, brain, and pituitary.