Light-sheet microscopy

Light-sheet microscopy uses a thin plane of light to illuminate a sample orthogonally to the detection objective such that the axial resolution of the microscope is determined wholly or partly by the thickness of the light sheet. It allows fast volumetric imaging with reduced sample irradiation compared to conventional light microscopy methods.

Latest Research and Reviews

  • Research |

    Light-sheet microscopy in the NIR-II window enables rapid volumetric imaging of tissues at impressive depths in vivo without invasive preparations owing to the reduced light scattering and tissue autofluorescence at these wavelengths.

    • Feifei Wang
    • , Hao Wan
    • , Zhuoran Ma
    • , Yeteng Zhong
    • , Qinchao Sun
    • , Ye Tian
    • , Liangqiong Qu
    • , Haotian Du
    • , Mingxi Zhang
    • , Lulin Li
    • , Huilong Ma
    • , Jian Luo
    • , Yongye Liang
    • , Wen Jung Li
    • , Guosong Hong
    • , Lianqing Liu
    •  & Hongjie Dai
    Nature Methods 16, 545-552
  • Research |

    Epi-illumination SPIM enables fast, volumetric, high-resolution, subcellular imaging of any sample compatible with a standard inverted fluorescence microscope.

    • Bin Yang
    • , Xingye Chen
    • , Yina Wang
    • , Siyu Feng
    • , Veronica Pessino
    • , Nico Stuurman
    • , Nathan H. Cho
    • , Karen W. Cheng
    • , Samuel J. Lord
    • , Linfeng Xu
    • , Dan Xie
    • , R. Dyche Mullins
    • , Manuel D. Leonetti
    •  & Bo Huang
    Nature Methods 16, 501-504
  • Research |

    An ex vivo 3D culture model of mycobacterial granulomas recapitulates the in vivo physiology of these structures and enables longitudinal imaging studies. The platform allows genetic and pharmacological manipulation of this key structure.

    • Mark R. Cronan
    • , Molly A. Matty
    • , Allison F. Rosenberg
    • , Landry Blanc
    • , Charlie J. Pyle
    • , Scott T. Espenschied
    • , John F. Rawls
    • , Véronique Dartois
    •  & David M. Tobin
    Nature Methods 15, 1098-1107
  • Research |

    Imaging of neuronal activity across the whole zebrafish brain in combination with online analysis allows for manipulating neuronal activity according to function. This approach is used to ablate or activate neurons in fictively swimming zebrafish larvae.

    • Nikita Vladimirov
    • , Chen Wang
    • , Burkhard Höckendorf
    • , Avinash Pujala
    • , Masashi Tanimoto
    • , Yu Mu
    • , Chao-Tsung Yang
    • , Jason D. Wittenbach
    • , Jeremy Freeman
    • , Stephan Preibisch
    • , Minoru Koyama
    • , Philipp J. Keller
    •  & Misha B. Ahrens
    Nature Methods 15, 1117-1125
  • Research | | open

    Sample orientation is crucial to ensure optimal image quality in light microscopy. Here the authors enable multi-axis orientation of fixed mouse embryos and shrimp, and live zebrafish embryos and larvae by introducing magnetic beads and rotating the sample with a magnetic field in a microscope.

    • Frederic Berndt
    • , Gopi Shah
    • , Rory M. Power
    • , Jan Brugués
    •  & Jan Huisken

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