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Invadopodia are dynamic actin-rich membrane protrusions that degrade the extracellular matrix through the local deposition of proteases and are involved in cell invasion. Invadopodia are found on cancer cells and are similar to the podosomes of normal cells.
This protocol describes a rapid (<24 h), reproducible and cheap technique for evaluating cancer cell invasiveness by quantifying in vivo rates of cancer cell extravasation in the chorioallantoic membrane (CAM) of chicken embryos.
Podosomes and invadopodia are actin-based dynamic protrusions of the plasma membrane of metazoan cells that represent sites of attachment to — and degradation of — the extracellular matrix. Progress has been made in our understanding of the regulation and function of these structures, and their role in human disease.