Industrial microbiology

  • Article
    | Open Access

    In lignocellulose biomass, microbes prefer consuming glucose over xylose, which affects target compound production. Here, the authors achieve simultaneous utilization of glucose and xylose for target chemical production and cell growth, respectively, and realize high-level production of shikimate pathway derivatives.

    • Ryosuke Fujiwara
    • , Shuhei Noda
    • , Tsutomu Tanaka
    •  & Akihiko Kondo
  • Article
    | Open Access

    Accounting for the effects of genetic expression in genome-scale metabolic models is challenging. Here, the authors introduce a model formulation that efficiently simulates thermodynamic-compliant fluxes, enzyme and mRNA concentration levels, allowing omics integration and broad analysis of in silico cellular physiology.

    • Pierre Salvy
    •  & Vassily Hatzimanikatis
  • Article
    | Open Access

    Spread of antimicrobial-resistant (AR) bacteria is a global concern, but contributing factors remain unclear. Here, authors analyze distribution of AR bacteria in households from three ethnic groups in Tanzania and find that livelihood factors are more strongly associated with AR prevalence than antibiotic use.

    • Murugan Subbiah
    • , Mark A. Caudell
    • , Colette Mair
    • , Margaret A. Davis
    • , Louise Matthews
    • , Robert J. Quinlan
    • , Marsha B. Quinlan
    • , Beatus Lyimo
    • , Joram Buza
    • , Julius Keyyu
    •  & Douglas R. Call
  • Article
    | Open Access

    Remdesivir (RDV) is a broad-spectrum antiviral drug with activity against MERS coronavirus, but in vivo efficacy has not been evaluated. Here, the authors show that RDV has superior anti-MERS activity in vitro and in vivo compared to combination therapy with lopinavir, ritonavir and interferon beta and reduces severe lung pathology.

    • Timothy P. Sheahan
    • , Amy C. Sims
    • , Sarah R. Leist
    • , Alexandra Schäfer
    • , John Won
    • , Ariane J. Brown
    • , Stephanie A. Montgomery
    • , Alison Hogg
    • , Darius Babusis
    • , Michael O. Clarke
    • , Jamie E. Spahn
    • , Laura Bauer
    • , Scott Sellers
    • , Danielle Porter
    • , Joy Y. Feng
    • , Tomas Cihlar
    • , Robert Jordan
    • , Mark R. Denison
    •  & Ralph S. Baric
  • Article
    | Open Access

    Bacterial biofilms rely on shared extracellular polymeric substances (EPS) and are often highly tolerant to antibiotics. Here, the authors show in in vitro experiments that Salmonella does not evolve resistance to EPS inhibition because such strains are outcompeted by a susceptible strain under inhibitor treatment.

    • Lise Dieltjens
    • , Kenny Appermans
    • , Maries Lissens
    • , Bram Lories
    • , Wook Kim
    • , Erik V. Van der Eycken
    • , Kevin R. Foster
    •  & Hans P. Steenackers
  • Article
    | Open Access

    The authors show that resistance to the antifungal 5-fluorocytosine in Cryptococcus deuterogattii is acquired more frequently in isolates with elevated mutation rate, and is associated with alterations in capsule biosynthesis and nucleotide metabolism.

    • R. Blake Billmyre
    • , Shelly Applen Clancey
    • , Lucy X. Li
    • , Tamara L. Doering
    •  & Joseph Heitman
  • Article
    | Open Access

    A robust platform to study modular polyketide synthases (PKSs) in vitro is still unavailable. Here, the authors report the reconstitution of the venemycin PKS, engineer hybrid venemycin/pikromycin PKSs, and obtain much improved yields through employing the updated module boundaries.

    • Takeshi Miyazawa
    • , Melissa Hirsch
    • , Zhicheng Zhang
    •  & Adrian T. Keatinge-Clay
  • Article
    | Open Access

    The secretory pathway is used in the production of most biopharmaceuticals, but the associated biosynthetic costs are little understood. Here, the authors integrate the core secretory pathway into genome-scale metabolic models of human, mouse, and CHO cells, enabling in silico analysis.

    • Jahir M. Gutierrez
    • , Amir Feizi
    • , Shangzhong Li
    • , Thomas B. Kallehauge
    • , Hooman Hefzi
    • , Lise M. Grav
    • , Daniel Ley
    • , Deniz Baycin Hizal
    • , Michael J. Betenbaugh
    • , Bjorn Voldborg
    • , Helene Faustrup Kildegaard
    • , Gyun Min Lee
    • , Bernhard O. Palsson
    • , Jens Nielsen
    •  & Nathan E. Lewis
  • Article
    | Open Access

    Newly acquired plasmids are frequently lost due to fitness costs. Here, Zhang et al. show that the evolution of satellite plasmids with gene deletions can reduce fitness costs by driving down the copy number of full plasmids and thus favor maintenance of the full plasmid and its novel accessory genes.

    • Xue Zhang
    • , Daniel E. Deatherage
    • , Hao Zheng
    • , Stratton J. Georgoulis
    •  & Jeffrey E. Barrick
  • Article
    | Open Access

    Genome-scale engineering is generally limited to single methods of alteration such as overexpression, repression or deletion. Here the authors present a tri-functional CRISPR system that can engineer complex synergistic interactions in a genome-wide manner.

    • Jiazhang Lian
    • , Carl Schultz
    • , Mingfeng Cao
    • , Mohammad HamediRad
    •  & Huimin Zhao
  • Article
    | Open Access

    The genetics underlying bacterial susceptibility to antimicrobial peptides (AMPs) remain unclear. Here, using a genome-wide chemical-genetic approach, the authors map the diversity of resistance determinants across AMPs in Escherichia coli and provide evidence that cross-resistance is prevalent only between AMPs with similar modes of action.

    • Bálint Kintses
    • , Pramod K. Jangir
    • , Gergely Fekete
    • , Mónika Számel
    • , Orsolya Méhi
    • , Réka Spohn
    • , Lejla Daruka
    • , Ana Martins
    • , Ali Hosseinnia
    • , Alla Gagarinova
    • , Sunyoung Kim
    • , Sadhna Phanse
    • , Bálint Csörgő
    • , Ádám Györkei
    • , Eszter Ari
    • , Viktória Lázár
    • , István Nagy
    • , Mohan Babu
    • , Csaba Pál
    •  & Balázs Papp
  • Article
    | Open Access

    Here, Bhattacharya et al. chemically mutagenize Leishmania and identify genes associated with resistance to miltefosine and paromomycin by next generation sequencing. The study shows that a protein kinase (CDPK1) can mediate resistance to paromomycin by affecting translation.

    • Arijit Bhattacharya
    • , Philippe Leprohon
    • , Sophia Bigot
    • , Prasad Kottayil Padmanabhan
    • , Angana Mukherjee
    • , Gaétan Roy
    • , Hélène Gingras
    • , Anais Mestdagh
    • , Barbara Papadopoulou
    •  & Marc Ouellette
  • Article
    | Open Access

    Tightly controlling cell output is challenging, which has limited development and applications of bacterial sensors. Here the authors develop tunable, fast-responding sensors to control production of metabolic pigments and use them to assess zinc deficiency in a low-cost, minimal equipment fashion.

    • Monica P. McNerney
    • , Cirstyn L. Michel
    • , Krishi Kishore
    • , Janet Standeven
    •  & Mark P. Styczynski
  • Article
    | Open Access

    The folding-dependent catalytic behavior of enzymes is of fundamental biological importance, yet mechanistically underexplored. Here, the authors show that an α-helix, commonly found in proteins, can facilitate its own growth through the self-catalyzed polymerization of N-carboxyanhydride in solvents with low polarity.

    • Ziyuan Song
    • , Hailin Fu
    • , Ryan Baumgartner
    • , Lingyang Zhu
    • , Kuo-Chih Shih
    • , Yingchun Xia
    • , Xuetao Zheng
    • , Lichen Yin
    • , Christophe Chipot
    • , Yao Lin
    •  & Jianjun Cheng
  • Article
    | Open Access

    Established bacterial glycoengineering platforms limit access to protein and glycan substrates. Here the authors design a cytoplasmic protein glycosylation system, Glycoli, to generate a variety of multivalent glycostructures.

    • Hanne L. P. Tytgat
    • , Chia-wei Lin
    • , Mikail D. Levasseur
    • , Markus B. Tomek
    • , Christoph Rutschmann
    • , Jacqueline Mock
    • , Nora Liebscher
    • , Naohiro Terasaka
    • , Yusuke Azuma
    • , Michael Wetter
    • , Martin F. Bachmann
    • , Donald Hilvert
    • , Markus Aebi
    •  & Timothy G. Keys
  • Article
    | Open Access

    The pathogenic bacterium Legionella pneumophila infects lung macrophages and environmental amoebae. Here, Personnic et al. show that the pathogen reversibly forms virulent, antibiotic-tolerant subpopulations during infection of macrophages and amoebae, in a process regulated by the Lqs quorum-sensing system.

    • Nicolas Personnic
    • , Bianca Striednig
    • , Emmanuelle Lezan
    • , Christian Manske
    • , Amanda Welin
    • , Alexander Schmidt
    •  & Hubert Hilbi
  • Article
    | Open Access

    Cytochrome bd oxidases couple quinol oxidation and the release of protons to the periplasmic side with proton uptake from the cytoplasmic side to reduce dioxygen to water and they are the terminal reductases in bacterial and archaeal respiratory chains. Here the authors present the cryo-EM structure of Escherichia coli bd oxidase and discuss mechanistic implications.

    • Alexander Theßeling
    • , Tim Rasmussen
    • , Sabrina Burschel
    • , Daniel Wohlwend
    • , Jan Kägi
    • , Rolf Müller
    • , Bettina Böttcher
    •  & Thorsten Friedrich
  • Article
    | Open Access

    Individual bacteria within isogenic populations can differ in antibiotic tolerance. Here, Libby et al. show that antibiotic tolerance variability can be driven by ‘noisy’ expression of a gene encoding a (p)ppGpp synthetase, which is in turn regulated by multisite phosphorylation of a transcription factor.

    • Elizabeth A. Libby
    • , Shlomi Reuveni
    •  & Jonathan Dworkin
  • Article
    | Open Access

    Existing efforts have been focused on one of the elements in the automation of the design, build, test, and learn (DBTL) cycle for biosystems design. Here, the authors integrate a robotic system with machine learning algorithms to fully automate the DBTL cycle and apply it in optimizing the lycopene biosynthetic pathway.

    • Mohammad HamediRad
    • , Ran Chao
    • , Scott Weisberg
    • , Jiazhang Lian
    • , Saurabh Sinha
    •  & Huimin Zhao
  • Article
    | Open Access

    Existing heavy metal bioremediation systems are mainly based on plants, which require long growing time in specific conditions. Here, the authors mimic the characteristics of plant hyperaccumulators to engineer more tractable baker’s yeast and achieve 10–100-fold higher accumulation of chromium, arsenic, or cadmium.

    • George L. Sun
    • , Erin. E. Reynolds
    •  & Angela M. Belcher
  • Article
    | Open Access

    Flexizymes have been used to expand the scope of chemical substrates for ribosome-directed polymerization in vitro. Here the authors deduce design rules of Flexizyme-mediated tRNA acylation that more effectively predict the incorporation of new monomers into peptides.

    • Joongoo Lee
    • , Kenneth E. Schwieter
    • , Andrew M. Watkins
    • , Do Soon Kim
    • , Hao Yu
    • , Kevin J. Schwarz
    • , Jongdoo Lim
    • , Jaime Coronado
    • , Michelle Byrom
    • , Eric V. Anslyn
    • , Andrew D. Ellington
    • , Jeffrey S. Moore
    •  & Michael C. Jewett
  • Article
    | Open Access

    Microbial production of aromatic amino acid (AAA)-derived chemicals remains an outstanding metabolic engineering challenge. Here, the authors engineer baker’s yeast for high levels p-coumaric acid production by rewiring the central carbon metabolism and channeling more flux to the AAA biosynthetic pathway.

    • Quanli Liu
    • , Tao Yu
    • , Xiaowei Li
    • , Yu Chen
    • , Kate Campbell
    • , Jens Nielsen
    •  & Yun Chen
  • Article
    | Open Access

    Engineering cell lines often requires multiple plasmids with different selection markers. Here the authors present SiMPl, a method based on rationally engineered split enzymes which get reconstituted via intein-mediated protein splicing to maintain two plasmids using a single antibiotic.

    • Navaneethan Palanisamy
    • , Anna Degen
    • , Anna Morath
    • , Jara Ballestin Ballestin
    • , Claudia Juraske
    • , Mehmet Ali Öztürk
    • , Georg A. Sprenger
    • , Jung-Won Youn
    • , Wolfgang W. Schamel
    •  & Barbara Di Ventura
  • Article
    | Open Access

    Engineering Taxol pathway in microbes needs to overcome the difficulty of expressing plant P450 enzymes. Here, the authors use a compartmentalized metabolic engineering strategy to construct the taxanes production pathway in chloroplasts of Nicotiana benthamiana and realize the production of taxadience-5α-ol.

    • Jianhua Li
    • , Ishmael Mutanda
    • , Kaibo Wang
    • , Lei Yang
    • , Jiawei Wang
    •  & Yong Wang
  • Article
    | Open Access

    Shigella sonnei is one of the main species causing shigellosis worldwide. Here the authors analyse nearly 400 S. sonnei genome sequences and carry out experimental evolution experiments to shed light into the evolutionary processes underlying the recent emergence of fluoroquinolone resistance in this pathogen.

    • Hao Chung The
    • , Christine Boinett
    • , Duy Pham Thanh
    • , Claire Jenkins
    • , Francois-Xavier Weill
    • , Benjamin P. Howden
    • , Mary Valcanis
    • , Niall De Lappe
    • , Martin Cormican
    • , Sonam Wangchuk
    • , Ladaporn Bodhidatta
    • , Carl J. Mason
    • , To Nguyen Thi Nguyen
    • , Tuyen Ha Thanh
    • , Vinh Phat Voong
    • , Vu Thuy Duong
    • , Phu Huong Lan Nguyen
    • , Paul Turner
    • , Ryan Wick
    • , Pieter-Jan Ceyssens
    • , Guy Thwaites
    • , Kathryn E. Holt
    • , Nicholas R. Thomson
    • , Maia A. Rabaa
    •  & Stephen Baker
  • Article
    | Open Access

    The authors report the synthesis of an enantiomeric block co-beta-peptide that kills methicillin-resistant Staphylococcus aureus, including biofilm and persister bacterial cells, and disperses biofilms. The copolymer displays antibacterial activity in human ex vivo and mouse in vivo infection models without toxicity.

    • Kaixi Zhang
    • , Yu Du
    • , Zhangyong Si
    • , Yang Liu
    • , Michelle E. Turvey
    • , Cheerlavancha Raju
    • , Damien Keogh
    • , Lin Ruan
    • , Subramanion L. Jothy
    • , Sheethal Reghu
    • , Kalisvar Marimuthu
    • , Partha Pratim De
    • , Oon Tek Ng
    • , José R. Mediavilla
    • , Barry N. Kreiswirth
    • , Yonggui Robin Chi
    • , Jinghua Ren
    • , Kam C. Tam
    • , Xue-Wei Liu
    • , Hongwei Duan
    • , Yabin Zhu
    • , Yuguang Mu
    • , Paula T. Hammond
    • , Guillermo C. Bazan
    • , Kevin Pethe
    •  & Mary B. Chan-Park
  • Article
    | Open Access

    The authors investigate the burden and spatiotemporal dynamics of multidrug-resistant bacteria on intensive care unit surfaces from two hospitals in the US and Pakistan over a year. They find Acinetobacter baumannii and Enterococcus faecium co-association, likely due to synergistic biofilm formation.

    • Alaric W. D’Souza
    • , Robert F. Potter
    • , Meghan Wallace
    • , Angela Shupe
    • , Sanket Patel
    • , Xiaoqing Sun
    • , Danish Gul
    • , Jennie H. Kwon
    • , Saadia Andleeb
    • , Carey-Ann D. Burnham
    •  & Gautam Dantas
  • Article
    | Open Access

    CRISPR nucleases can be programmed to cleave sequences in specific bacteria to induce cell death. Here, Hamilton et al. present an optimized method for conjugative delivery of CRISPR nucleases, consisting of a single plasmid that encodes both the conjugative machinery and the nuclease.

    • Thomas A. Hamilton
    • , Gregory M. Pellegrino
    • , Jasmine A. Therrien
    • , Dalton T. Ham
    • , Peter C. Bartlett
    • , Bogumil J. Karas
    • , Gregory B. Gloor
    •  & David R. Edgell
  • Article
    | Open Access

    Antimicrobial peptides (AMPs) are emerging as drug candidates, but the risk of pathogen resistance is not well understood. Here, the authors investigate AMP resistance evolution in E. coli, finding physicochemical features that make AMPs less prone to resistance and no cross- or horizontally-acquired resistance.

    • Réka Spohn
    • , Lejla Daruka
    • , Viktória Lázár
    • , Ana Martins
    • , Fanni Vidovics
    • , Gábor Grézal
    • , Orsolya Méhi
    • , Bálint Kintses
    • , Mónika Számel
    • , Pramod K. Jangir
    • , Bálint Csörgő
    • , Ádám Györkei
    • , Zoltán Bódi
    • , Anikó Faragó
    • , László Bodai
    • , Imre Földesi
    • , Diána Kata
    • , Gergely Maróti
    • , Bernadett Pap
    • , Roland Wirth
    • , Balázs Papp
    •  & Csaba Pál
  • Article
    | Open Access

    Livestock play a role in maintaining antibiotic resistance reservoirs. Here, Liu et al. use metagenomics to study the dynamics of antibiotic resistance genes (ARGs) in the gut of dairy cattle during early life, showing that colostrum is a potential source of ARGs and ARG abundance declines during nursing covarying with dietary transition.

    • Jinxin Liu
    • , Diana H. Taft
    • , Maria X. Maldonado-Gomez
    • , Daisy Johnson
    • , Michelle L. Treiber
    • , Danielle G. Lemay
    • , Edward J. DePeters
    •  & David A. Mills
  • Article
    | Open Access

    Antibiotic resistance is a major global health challenge. Here, the authors report on a thermoresponsive delivery system for combined photothermal and antibiotic delivery with fluorescent tracking abilities and demonstrate application against antibiotic resistant bacteria in vitro and in vivo.

    • Guangchao Qing
    • , Xianxian Zhao
    • , Ningqiang Gong
    • , Jing Chen
    • , Xianlei Li
    • , Yaling Gan
    • , Yongchao Wang
    • , Zhen Zhang
    • , Yuxuan Zhang
    • , Weisheng Guo
    • , Yang Luo
    •  & Xing-Jie Liang
  • Article
    | Open Access

    Here, a cross of Plasmodium vivax malaria parasites links a chloroquine resistance (CQR) phenotype to a 76 kb region of chromosome 1 and greater expression of pvcrt, an ortholog of the Plasmodium falciparum CQR transporter gene.

    • Juliana M. Sá
    • , Sarah R. Kaslow
    • , Roberto R. Moraes Barros
    • , Nicholas F. Brazeau
    • , Christian M. Parobek
    • , Dingyin Tao
    • , Rebecca E. Salzman
    • , Tyler J. Gibson
    • , Soundarapandian Velmurugan
    • , Michael A. Krause
    • , Viviana Melendez-Muniz
    • , Whitney A. Kite
    • , Paul K. Han
    • , Richard T. Eastman
    • , Adam Kim
    • , Evan G. Kessler
    • , Yonas Abebe
    • , Eric R. James
    • , Sumana Chakravarty
    • , Sachy Orr-Gonzalez
    • , Lynn E. Lambert
    • , Theresa Engels
    • , Marvin L. Thomas
    • , Pius S. Fasinu
    • , David Serre
    • , Robert W. Gwadz
    • , Larry Walker
    • , Derrick K. DeConti
    • , Jianbing Mu
    • , Jeffrey A. Bailey
    • , B. Kim Lee Sim
    • , Stephen L. Hoffman
    • , Michael P. Fay
    • , Rhoel R. Dinglasan
    • , Jonathan J. Juliano
    •  & Thomas E. Wellems
  • Article
    | Open Access

    Power densities of existing microbial biophotovoltaics (BPV) are low and unendurable. Here, the authors develop a BPV based on d-lactate mediated microbial consortium, which can generate an average power density of 135 mW·m−2 for over 40 days in a spatial-temporal separation setup with medium replenishment.

    • Huawei Zhu
    • , Hengkai Meng
    • , Wei Zhang
    • , Haichun Gao
    • , Jie Zhou
    • , Yanping Zhang
    •  & Yin Li
  • Article
    | Open Access

    Invasive non-typhoidal Salmonella (iNTS) infections are dominated by antibiotic resistant isolates of the sequence type (ST) 313. Here, the authors identify the ST313 sublineage II.1 in the Democratic Republic of the Congo exhibiting extensive drug resistance and genetic signatures potentially associated with host adaptation.

    • Sandra Van Puyvelde
    • , Derek Pickard
    • , Koen Vandelannoote
    • , Eva Heinz
    • , Barbara Barbé
    • , Tessa de Block
    • , Simon Clare
    • , Eve L. Coomber
    • , Katherine Harcourt
    • , Sushmita Sridhar
    • , Emily A. Lees
    • , Nicole E. Wheeler
    • , Elizabeth J. Klemm
    • , Laura Kuijpers
    • , Lisette Mbuyi Kalonji
    • , Marie-France Phoba
    • , Dadi Falay
    • , Dauly Ngbonda
    • , Octavie Lunguya
    • , Jan Jacobs
    • , Gordon Dougan
    •  & Stijn Deborggraeve
  • Article
    | Open Access

    FK506 is a potential antifungal compound that inhibits calcineurin, but it also has immunosuppressive activity. Here, Juvvadi et al. report the structure of FK506 in complex with the FK506-binding protein FKPB12 and calcineurin, and design a less immunosuppresive FK506 analog with antifungal activity in mice.

    • Praveen R. Juvvadi
    • , David Fox III
    • , Benjamin G. Bobay
    • , Michael J. Hoy
    • , Sophie M. C. Gobeil
    • , Ronald A. Venters
    • , Zanetta Chang
    • , Jackie J. Lin
    • , Anna Floyd Averette
    • , D. Christopher Cole
    • , Blake C. Barrington
    • , Joshua D. Wheaton
    • , Maria Ciofani
    • , Michael Trzoss
    • , Xiaoming Li
    • , Soo Chan Lee
    • , Ying-Lien Chen
    • , Mitchell Mutz
    • , Leonard D. Spicer
    • , Maria A. Schumacher
    • , Joseph Heitman
    •  & William J. Steinbach
  • Article
    | Open Access

    Metabolic enzymes often form supramolecular complexes to improve product yield. Here the authors use short peptide tags to create scaffold-free assemblies and synthetic metabolic nodes.

    • Wei Kang
    • , Tian Ma
    • , Min Liu
    • , Jiale Qu
    • , Zhenjun Liu
    • , Huawei Zhang
    • , Bin Shi
    • , Shuai Fu
    • , Juncai Ma
    • , Louis Tung Faat Lai
    • , Sicong He
    • , Jianan Qu
    • , Shannon Wing-Ngor Au
    • , Byung Ho Kang
    • , Wilson Chun Yu Lau
    • , Zixin Deng
    • , Jiang Xia
    •  & Tiangang Liu
  • Article
    | Open Access

    Alkaline lakes have some of the highest productivity rates in freshwater ecosystems. Here the authors report amplicon, metagenome, and proteome sequencing from microbial mat communities of four alkaline lakes in Canada, and compare these lakes to central Asian soda lakes, revealing a shared core microbiome despite the geographical distance.

    • Jackie K. Zorz
    • , Christine Sharp
    • , Manuel Kleiner
    • , Paul M. K. Gordon
    • , Richard T. Pon
    • , Xiaoli Dong
    •  & Marc Strous
  • Article
    | Open Access

    D-cycloserine (DCS) has been used for decades to treat Mycobacterium tuberculosis (Mtb) but resistance is rarely observed in clinical isolates. Here, the authors report ultra-low rate of emergence of resistance mutations as the underlying mechanism of DCS resistance evasion in Mtb.

    • Dimitrios Evangelopoulos
    • , Gareth A. Prosser
    • , Angela Rodgers
    • , Belinda M. Dagg
    • , Bhagwati Khatri
    • , Mei Mei Ho
    • , Maximiliano G. Gutierrez
    • , Teresa Cortes
    •  & Luiz Pedro S. de Carvalho
  • Article
    | Open Access

    Anthropogenic CO2 emissions are causing ocean acidification, which can affect the physiology of some organisms. Here, Botté et al. use metagenomics to show differences in metabolic potential between sponge microbiomes sampled at a shallow volcanic CO2 seep and those from nearby control sites.

    • Emmanuelle S. Botté
    • , Shaun Nielsen
    • , Muhammad Azmi Abdul Wahab
    • , John Webster
    • , Steven Robbins
    • , Torsten Thomas
    •  & Nicole S. Webster
  • Article
    | Open Access

    Microbial communities of plant leaf surfaces are ecologically important, but how they assemble and vary in time is unclear. Here, the authors identify core leaf microbiomes and seasonal patterns for two biofuel crops and show with source-sink models that soil is a reservoir of phyllosphere diversity.

    • Keara L. Grady
    • , Jackson W. Sorensen
    • , Nejc Stopnisek
    • , John Guittar
    •  & Ashley Shade
  • Article
    | Open Access

    The authors present multifunctional nanoagents that can be used for imaging and treatment of bacterial infections. The nanoagents are made of fluorescent silicon nanoparticles functionalized with a glucose polymer and loaded with chlorin e6, and are taken up by bacteria via ABC transporters.

    • Jiali Tang
    • , Binbin Chu
    • , Jinhua Wang
    • , Bin Song
    • , Yuanyuan Su
    • , Houyu Wang
    •  & Yao He
  • Article
    | Open Access

    Sustainable sources are needed to meet the demand for long-chain polyunsaturated fatty acids. Here the authors construct an artificial biosynthetic gene cluster in Y. lipolytica capable of producing a high concentration of PUFAs.

    • Katja Gemperlein
    • , Demian Dietrich
    • , Michael Kohlstedt
    • , Gregor Zipf
    • , Hubert S. Bernauer
    • , Christoph Wittmann
    • , Silke C. Wenzel
    •  & Rolf Müller
  • Article
    | Open Access

    The International Space Station is a unique habitat for humans and microbes. Here, Mora et al. analyze microbial communities from several areas aboard, finding similarities with those of ground-based indoor environments, as well as adaptations towards biofilm formation but not necessarily relevant to human health.

    • Maximilian Mora
    • , Lisa Wink
    • , Ines Kögler
    • , Alexander Mahnert
    • , Petra Rettberg
    • , Petra Schwendner
    • , René Demets
    • , Charles Cockell
    • , Tatiana Alekhova
    • , Andreas Klingl
    • , Robert Krause
    • , Anna Zolotariof
    • , Alina Alexandrova
    •  & Christine Moissl-Eichinger
  • Article
    | Open Access

    Here, Williamson et al. combine epidemiological and genomic analysis of 2,186 Neisseria gonorrhoeae isolates from Australia and show that men who have sex with men and women are a possible ‘bridging’ population between men who have sex with men and heterosexuals.

    • Deborah A. Williamson
    • , Eric P. F. Chow
    • , Claire L. Gorrie
    • , Torsten Seemann
    • , Danielle J. Ingle
    • , Nasra Higgins
    • , Marion Easton
    • , George Taiaroa
    • , Yonatan H. Grad
    • , Jason C. Kwong
    • , Christopher K. Fairley
    • , Marcus Y. Chen
    •  & Benjamin P. Howden
  • Article
    | Open Access

    The wealth of complex polyketides is an essential source for drug discovery. Here, the authors report an evolution-guided rational morphing of modular polyketide synthases (PKSs) for aurothin and neoaurothin biosynthesis, and reveal engineering site suitable for diversifying PKS systems.

    • Huiyun Peng
    • , Keishi Ishida
    • , Yuki Sugimoto
    • , Holger Jenke-Kodama
    •  & Christian Hertweck