Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer).
Glycomics is a subset of the field of glycobiology that aims to identify the structure and function of the complete set of glycans (the glycome) produced in a given cell or organism and identify all the genes that encode glycoproteins.
The comprehensive study of protein glycosylation has been complicated by the complex structural diversity of glycans. In this protocol, Yang et al. describe a solid-phase method for the sequential analysis of N-linked and O-linked glycans.
The analysis of site-specific glycosylation of HIV Envelope glycoprotein (Env) is challenging as it contains 25–30 glycosylation sites with multiple glycan forms at each site. Here the authors present a generally applicable mass spectrometry-based method for site-specific analysis of protein glycosylation that they apply to the analysis of the HIV-1 Env.