Glycobiology

Definition

Glycobiology is the study of the structure, biosynthesis and biological functions of carbohydrates.

Latest Research and Reviews

  • Research | | open

    IgG glycosylation is an important factor in immune function, yet the molecular details of protein glycosylation remain poorly understood. The data-driven approach presented here uses large-scale plasma IgG mass spectrometry measurements to infer new biochemical reactions in the glycosylation pathway.

    • Elisa Benedetti
    • , Maja Pučić-Baković
    • , Toma Keser
    • , Annika Wahl
    • , Antti Hassinen
    • , Jeong-Yeh Yang
    • , Lin Liu
    • , Irena Trbojević-Akmačić
    • , Genadij Razdorov
    • , Jerko Štambuk
    • , Lucija Klarić
    • , Ivo Ugrina
    • , Maurice H. J. Selman
    • , Manfred Wuhrer
    • , Igor Rudan
    • , Ozren Polasek
    • , Caroline Hayward
    • , Harald Grallert
    • , Konstantin Strauch
    • , Annette Peters
    • , Thomas Meitinger
    • , Christian Gieger
    • , Marija Vilaj
    • , Geert-Jan Boons
    • , Kelley W. Moremen
    • , Tatiana Ovchinnikova
    • , Nicolai Bovin
    • , Sakari Kellokumpu
    • , Fabian J. Theis
    • , Gordan Lauc
    •  & Jan Krumsiek
  • Research | | open

    SIRT1 is a stress sensor whose deacetylase activity is increased during cellular stress, but the molecular mechanism is unclear. Here, the authors show that O-GlcNAcylation of SIRT1 is elevated upon different stress stimuli and increases SIRT1 deacetylase activity, protecting cells from stress-induced apoptosis.

    • Cuifang Han
    • , Yuchao Gu
    • , Hui Shan
    • , Wenyi Mi
    • , Jiahui Sun
    • , Minghui Shi
    • , Xinling Zhang
    • , Xinzhi Lu
    • , Feng Han
    • , Qianhong Gong
    •  & Wengong Yu
  • Protocols |

    Conjugation of oligosaccharides to probes and surfaces is useful in the development of biochemical assays to assess carbohydrate–protein interactions. In this protocol, conjugation is enabled by using a bifunctional oligo(ethylene glycol) linker.

    • Christian T Hjuler
    • , Nicolai N Maolanon
    • , Jørgen Sauer
    • , Jens Stougaard
    • , Mikkel B Thygesen
    •  & Knud J Jensen
    Nature Protocols 12, 2411–2422

News and Comment