Genomic engineering

Genomic engineering is the synthetic assembly of complete chromosomal DNA that is more or less derived from natural genomic sequences. Genomic engineering is the top-down, global approach to synthetic biology; to be distinguished from bottom-up, local genetic circuit engineering.

Latest Research and Reviews

  • Research | | open

    Genome structural variation can play an important functional role in phenotypic diversity. Here the authors use the SCRaMbLE system on a ring synthetic chromosome V to generate complex rearrangements distinct from a rearranged linear chromosome.

    • Juan Wang
    • , Ze-Xiong Xie
    • , Yuan Ma
    • , Xiang-Rong Chen
    • , Yao-Qing Huang
    • , Bo He
    • , Bin Jia
    • , Bing-Zhi Li
    •  & Ying-Jin Yuan
  • Research | | open

    The Sc2.0 project has built the Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) system into their synthetic chromosomes. Here the authors use SCRaMbLE to rapidly develop, diversify and screen strains for diverse production and growth characteristics.

    • B. A. Blount
    • , G-O. F. Gowers
    • , J. C. H. Ho
    • , R. Ledesma-Amaro
    • , D. Jovicevic
    • , R. M. McKiernan
    • , Z. X. Xie
    • , B. Z. Li
    • , Y. J. Yuan
    •  & T. Ellis
  • Research | | open

    Pathway optimization and chassis engineering are usually carried out in a step-wise and trial-and-error manner. Here the authors present ’SCRaMbLE-in’ that combines in-vitro pathway rapid prototyping with in-vivo genome integration and optimization.

    • Wei Liu
    • , Zhouqing Luo
    • , Yun Wang
    • , Nhan T. Pham
    • , Laura Tuck
    • , Irene Pérez-Pi
    • , Longying Liu
    • , Yue Shen
    • , Chris French
    • , Manfred Auer
    • , Jon Marles-Wright
    • , Junbiao Dai
    •  & Yizhi Cai
  • Research | | open

    SCRaMbLE has been used to rearrange synthetic chromosomes that have been introduced into host yeast. Here the authors produce semi-synthetic heterozygous diploid strains for rapid selection of phenotypes and map the rearrangements underlying selected phenotypes such as thermoresistance and caffeine resistance.

    • Michael J. Shen
    • , Yi Wu
    • , Kun Yang
    • , Yunxiang Li
    • , Hui Xu
    • , Haoran Zhang
    • , Bing-Zhi Li
    • , Xia Li
    • , Wen-Hai Xiao
    • , Xiao Zhou
    • , Leslie A. Mitchell
    • , Joel S. Bader
    • , Yingjin Yuan
    •  & Jef D. Boeke

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