Genetic techniques

Genetic techniques include all methods used to study genetic phenomena such as heredity, variation and DNA structure and function.

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Latest Research and Reviews

  • Research | | open

    Dimitre Simeonov, Alexander Brandt et al. report a pathogenic bystander mutation caused by unintended repair of a CRISPR-Cas9-mediated deletion in mice. They generate mice lacking an IL2RA intronic enhancer previously associated with human disease risk and find that one line of edited mice show unexpected disease features due to a bystander mutation.

    • Dimitre R. Simeonov
    • , Alexander J. Brandt
    • , Alice Y. Chan
    • , Jessica T. Cortez
    • , Zhongmei Li
    • , Jonathan M. Woo
    • , Youjin Lee
    • , Claudia M. B. Carvalho
    • , Alyssa C. Indart
    • , Theodore L. Roth
    • , James Zou
    • , Andrew P. May
    • , James R. Lupski
    • , Mark S. Anderson
    • , F. William Buaas
    • , Daniel S. Rokhsar
    •  & Alexander Marson
  • Research |

    Fate-mapping and clonal analysis show that bronchioalveolar stem cells (BASCs) become activated and respond distinctly to different lung injuries and differentiate into multiple cell types. Single-cell RNA-seq analysis identifies new BASC markers.

    • Qiaozhen Liu
    • , Kuo Liu
    • , Guizhong Cui
    • , Xiuzhen Huang
    • , Shun Yao
    • , Wenke Guo
    • , Zhen Qin
    • , Yan Li
    • , Rui Yang
    • , Wenjuan Pu
    • , Libo Zhang
    • , Lingjuan He
    • , Huan Zhao
    • , Wei Yu
    • , Muxue Tang
    • , Xueying Tian
    • , Dongqing Cai
    • , Yu Nie
    • , Shengshou Hu
    • , Tao Ren
    • , Zengyong Qiao
    • , Hefeng Huang
    • , Yi Arial Zeng
    • , Naihe Jing
    • , Guangdun Peng
    • , Hongbin Ji
    •  & Bin Zhou
  • Research | | open

    Analogue regulation of gene expression is important for normal function in mammals but existing genetic technologies are designed to achieve ON/OFF control. Here the authors develop synthetic microRNA silencing-mediated fine-tuners (miSFITs) to precisely control target gene expression levels.

    • Yale S. Michaels
    • , Mike B. Barnkob
    • , Hector Barbosa
    • , Toni A. Baeumler
    • , Mary K. Thompson
    • , Violaine Andre
    • , Huw Colin-York
    • , Marco Fritzsche
    • , Uzi Gileadi
    • , Hilary M. Sheppard
    • , David J. H. F. Knapp
    • , Thomas A. Milne
    • , Vincenzo Cerundolo
    •  & Tudor A. Fulga
  • Research | | open

    Triple negative breast cancers (TNBC) disseminate and metastasise, but the clonal relationship of metastases to primary tumours is poorly understood. Here, the authors use cellular barcoding of TNBC patient-derived xenografts and track the fate of barcoded clones in primary tumours and their metastases, including after resection or chemotherapy.

    • D. Merino
    • , T. S. Weber
    • , A. Serrano
    • , F. Vaillant
    • , K. Liu
    • , B. Pal
    • , L. Di Stefano
    • , J. Schreuder
    • , D. Lin
    • , Y. Chen
    • , M. L. Asselin-Labat
    • , T. N. Schumacher
    • , D. Cameron
    • , G. K. Smyth
    • , A. T. Papenfuss
    • , G. J. Lindeman
    • , J. E. Visvader
    •  & S. H. Naik

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