Gene targeting articles from across Nature Portfolio

The delivery of CRISPR RNPs has potential advantages over other genome editing approaches, including reduced off-target editing and reduced immunogenicity. Here the authors report self-deliverable Cas9 RNPs capable of robustly editing cultured cells in vitro and the mouse brain upon direct injections.

Existing TadA-derived CBEs exhibit residual A•T-to-G•C editing activity and suffer from lower activity at several sequence contexts and with non-SpCas9 targeting domains. Here, the authors use phage-assisted evolution to evolve CBE6 variants that address these limitations.

Exo-PE is an approach to improve prime editing efficacy for insertions while maintaining precision.

An optimized zebrafish gene-tagging strategy was developed to enable easy and high-efficiency knock-in (KI), high odds to obtain seamless KI germlines, and sensitive in vivo tracing of proteins with different abundance.

Culex mosquitoes are carriers of major diseases like West Nile virus and are a public health concern. Here the authors present a CRISPR-Cas9 gene drive as a control technology in the Culex quinquefasciatus mosquito species.

A system that edits RNA rather than DNA can give new life to exhausted CAR T cells.

Two RNA-editing therapies for genetic diseases have in the past few months gained approval for clinical trials, raising hopes for safer treatments.

Immune cells armed with a mutation first identified in cancer cells gain potency but don’t turn cancerous themselves.

Scientists in the global south use the popular technique to protect local crops against local threats.

A study published in Science develops an efficient mining algorithm to identify and then experimentally characterize many rare CRISPR systems.