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Sun et al. describe how to image and quantify mitophagy in both living cells and tissues, using the pH-sensitive fluorescent reporter mt-Keima. This protocol provides information for analysis by both confocal and super-resolution microscopy.
To quantify transcription factor (TF)–DNA binding dynamics in vivo, this protocol describes how to perform photoactivatable fluorescence correlation spectroscopy in live mouse embryos via selective photoactivation of a subpopulation of TF molecules.
Genetically encoded iNap sensors allow imaging of NADPH with high spatiotemporal resolution in living systems. The iNaps cover physiologically relevant NADPH concentrations and are demonstrated in mammalian cells and live zebrafish.