Fluorescent labelling articles from across Nature Portfolio

Temporally sorting EV populations is hard. Here the authors report a microfluidic-based strategy to enable selective isolation of nascent EVs by using azido groups to act as a timestamp for click chemistry labelling: they apply this to mouse models of anti-PD-L1 immunotherapy.

Methods to detect and distinguish the early stage of viral infection often involve complicated and time-consuming protocols. Here, the authors disclose a class of fluorescent molecules that enable fast detection of avian influenza virus infection by selectively localizing at the endoplasmic reticulum in the cell.

Live cells and tissues are imaged over long time periods using a fast, non-toxic click chemistry.

Molecules that target mitochondria have promising applications as anticancer agents. Here quinolizinium and benzimidazolium cations are shown to deactivate mitochondria through two-photon absorption in the near infrared range.

Covalent functionalization of single-walled carbon nanotubes with luminescent sp³-defects generally produces a variety of binding configurations and emission wavelengths. The authors propose a base-mediated nucleophilic functionalization approach to selectively achieve configurations for E₁₁* and E₁₁*^- or purely E₁₁*^- defect emission.

The concept of massively parallel single-molecule protein sequencing emerges.