Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer).
Advanced diagnostic probes are required for monitoring disease progression. Here Galanzha et al. demonstrate a 22 nm plasmonic nanolaser to serve as a super-bright, biocompatible probe capable of generating stimulated emission directly inside living cells and animal tissue, while targeting cancer cells.
This protocol from Wang et al. describes a pulse–chase method to investigate autophagic protein degradation through click labeling of long-lived proteins. This is a safer alternative to similar classic methods that use radioactive labeling.
Site-specific labeling of proteins with small fluorophores is advantageous for imaging. Lemke et al. describe how to site-specifically label membrane proteins with organic fluorophores by incorporating non-canonical amino acids via Amber suppression technology.