Fluorescence in situ hybridization

Fluorescence in situ hybridization (FISH) is a method to localize nucleic acid targets in fixed cells for cytogenetic or gene expression studies. It relies on fluorophore-labelled DNA or RNA probes to count and localize specific genes or regions along chromosomes, detect mutations, or analyze temporal and spatial gene expression.

Latest Research and Reviews

  • Research | | open

    Wu et al. introduce RollFISH, a method that enables quantification of single-molecule RNA with high specificity and sensitivity by combining smFISH with rolling circle amplification. RollFISH facilitated studying heterogeneity of biomarkers in formalin-fixed and paraffin-embedded breast cancer tissue, demonstrating its clinical application.

    • Chenglin Wu
    • , Michele Simonetti
    • , Carla Rossell
    • , Marco Mignardi
    • , Reza Mirzazadeh
    • , Laura Annaratone
    • , Caterina Marchiò
    • , Anna Sapino
    • , Magda Bienko
    • , Nicola Crosetto
    •  & Mats Nilsson
  • Research | | open

    Automated analysis of RNA localisation in smFISH data has been elusive. Here, the authors simulate and use a large dataset of images to design and validate a framework for highly accurate classification of sub-cellular RNA localisation patterns from smFISH experiments.

    • Aubin Samacoits
    • , Racha Chouaib
    • , Adham Safieddine
    • , Abdel-Meneem Traboulsi
    • , Wei Ouyang
    • , Christophe Zimmer
    • , Marion Peter
    • , Edouard Bertrand
    • , Thomas Walter
    •  & Florian Mueller
  • Research | | open

    FISH-based techniques to image and count mRNA in single cells can be limited by the photophysical properties of organic dyes. Here the authors develop photostable quantum dot FISH probes for multiplexed imaging.

    • Yang Liu
    • , Phuong Le
    • , Sung Jun Lim
    • , Liang Ma
    • , Suresh Sarkar
    • , Zhiyuan Han
    • , Stephen J. Murphy
    • , Farhad Kosari
    • , George Vasmatzis
    • , John C. Cheville
    •  & Andrew M. Smith
  • Research |

    The ProximID approach generates single-cell expression profiles and a network of enriched physical cellular interactions within a tissue.

    • Jean-Charles Boisset
    • , Judith Vivié
    • , Dominic Grün
    • , Mauro J. Muraro
    • , Anna Lyubimova
    •  & Alexander van Oudenaarden
    Nature Methods 15, 547-553

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