Electron microscopy

Electron microscopy uses a beam of electrons to illuminate a sample and achieve much higher spatial resolution than light microscopy. Transmission electron microscopy generates an image of the internal structure of a thin sample. Scanning electron microscopy generates a topological image of a sample.

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Latest Research and Reviews

  • Research | | open

    During stress, protein synthesis is inhibited through phosphorylation of the initiation factor eIF2 on its alpha subunit and its interaction with eIF2B. Here the authors describe a structure of the yeast eIF2B in complex with its substrate - the GDP-bound phosphorylated eIF2, providing insights into how phosphorylation results in a tighter interaction with eIF2B.

    • Yuliya Gordiyenko
    • , José Luis Llácer
    •  & V. Ramakrishnan
  • Research | | open

    Multidrug efflux pumps actively expel a wide range of toxic substrates from bacteria and play a major role in drug resistance. Here authors show the in situ structure of the efflux pump AcrAB-TolC obtained by electron cryo-tomography and subtomogram averaging.

    • Xiaodong Shi
    • , Muyuan Chen
    • , Zhili Yu
    • , James M. Bell
    • , Hans Wang
    • , Isaac Forrester
    • , Heather Villarreal
    • , Joanita Jakana
    • , Dijun Du
    • , Ben F. Luisi
    • , Steven J. Ludtke
    •  & Zhao Wang
  • Research | | open

    Epsilon toxin (Etx) is a potent pore forming toxin (PFT) produced by Clostridium perfringens. Here authors show the cryo-EM structure of the Etx pore assembled on the membrane of susceptible cells and shed light on pore formation and mutant phenotypes.

    • Christos G. Savva
    • , Alice R. Clark
    • , Claire E. Naylor
    • , Michel R. Popoff
    • , David S. Moss
    • , Ajit K. Basak
    • , Richard W. Titball
    •  & Monika Bokori-Brown
  • Research | | open

    Ribonucleotide reductase (RNR) catalyzes the conversion of ribonucleotides to deoxyribonucleotides, which is an essential step in DNA synthesis. Here the authors use small-angle X-ray scattering, X-ray crystallography, and cryo-electron microscopy to capture active and inactive forms of the Bacillus subtilis RNR and provide mechanistic insights into a convergent form of allosteric regulation.

    • William C. Thomas
    • , F. Phil Brooks III
    • , Audrey A. Burnim
    • , John-Paul Bacik
    • , JoAnne Stubbe
    • , Jason T. Kaelber
    • , James Z. Chen
    •  & Nozomi Ando
  • Research | | open

    Ribonulease P is a conserved ribozyme present in all kingdoms of life that is involved in the 5′ maturation step of tRNAs. Here the authors determine the structure of an archaeal RNase P holoenzyme that reveals how archaeal RNase P recognizes its tRNA substrate and suggest a conserved catalytic mechanism amongst RNase Ps despite structural variability.

    • Futang Wan
    • , Qianmin Wang
    • , Jing Tan
    • , Ming Tan
    • , Juan Chen
    • , Shaohua Shi
    • , Pengfei Lan
    • , Jian Wu
    •  & Ming Lei
  • Research | | open

    Translation termination is under strong selection pressure for high speed and accuracy. Here the authors provide a 3D view of the dynamics of a translating bacterial ribosome as it recruits a class-1 release factor (RF1 or RF2) upon encountering a stop codon, and propose a structure-based kinetic model for the early steps in bacterial translation termination.

    • Ziao Fu
    • , Gabriele Indrisiunaite
    • , Sandip Kaledhonkar
    • , Binita Shah
    • , Ming Sun
    • , Bo Chen
    • , Robert A. Grassucci
    • , Måns Ehrenberg
    •  & Joachim Frank

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