Electron microscopy

Definition

Electron microscopy uses a beam of electrons to illuminate a sample and achieve much higher spatial resolution than light microscopy. Transmission electron microscopy generates an image of the internal structure of a thin sample. Scanning electron microscopy generates a topological image of a sample.

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Latest Research and Reviews

  • Research |

    Cryo-electron microscopy analysis of yeast Rad26 bound to RNA polymerase II provides insight into the initiation of the transcription-coupled DNA repair mechanism in eukaryotes.

    • Jun Xu
    • , Indrajit Lahiri
    • , Wei Wang
    • , Adam Wier
    • , Michael A. Cianfrocco
    • , Jenny Chong
    • , Alissa A. Hare
    • , Peter B. Dervan
    • , Frank DiMaio
    • , Andres E. Leschziner
    •  & Dong Wang
  • Research | | open

    Immunoproteasome selective inhibitors might lead to less toxic drugs for treatment of multiple myeloma and graft rejection. Here, the authors develop immunoproteasome specific asparagine-ethylenediamine (AsnEDA)-based inhibitory compounds, demonstrate their efficacy and present the AsnEDA bound immunoproteasome cryo-EM structure.

    • Ruda de Luna Almeida Santos
    • , Lin Bai
    • , Pradeep K. Singh
    • , Naoka Murakami
    • , Hao Fan
    • , Wenhu Zhan
    • , Yingrong Zhu
    • , Xiuju Jiang
    • , Kaiming Zhang
    • , Jean Pierre Assker
    • , Carl F. Nathan
    • , Huilin Li
    • , Jamil Azzi
    •  & Gang Lin
  • Research |

    A high-resolution structure of the human ribosome determined by cryo-electron microscopy visualizes numerous RNA modifications that are concentrated at functional sites with an extended shell, and suggests the possibility of designing more specific ribosome-targeting drugs.

    • S. Kundhavai Natchiar
    • , Alexander G. Myasnikov
    • , Hanna Kratzat
    • , Isabelle Hazemann
    •  & Bruno P. Klaholz
    Nature 551, 472–477
  • Research | | open

    Leishmaniasis is a parasitic disease transmitted by the bite of infected sand flies. Here the authors describe an atomic resolution cryo-EM structure of the Leishmania ribosome in complex with the recently approved drug paromomycin (PAR) and highlight conserved elements in the drug binding pocket that mediate PAR deleterious effects on the parasite.

    • Moran Shalev-Benami
    • , Yan Zhang
    • , Haim Rozenberg
    • , Yuko Nobe
    • , Masato Taoka
    • , Donna Matzov
    • , Ella Zimmerman
    • , Anat Bashan
    • , Toshiaki Isobe
    • , Charles L. Jaffe
    • , Ada Yonath
    •  & Georgios Skiniotis
  • Research | | open

    The transcription co-activator complex SAGA is recruited to promoters by transcriptional activators and promotes the formation of the pre-initiation complex. Here, the authors present the cryo-EM structure of the SAGA complex and resolve the major target of activator binding, the 430‚ÄČkDa Tra1 protein.

    • Grigory Sharov
    • , Karine Voltz
    • , Alexandre Durand
    • , Olga Kolesnikova
    • , Gabor Papai
    • , Alexander G. Myasnikov
    • , Annick Dejaegere
    • , Adam Ben Shem
    •  & Patrick Schultz
  • Research | | open

    tRNA-dependent cysteine biosynthesis is catalyzed by the transsulfursome protein complex. Here, the authors use a multidisciplinary approach to structurally characterize the archaeal transsulfursome and propose a model for tRNA channeling in the complex.

    • Meirong Chen
    • , Koji Kato
    • , Yume Kubo
    • , Yoshikazu Tanaka
    • , Yuchen Liu
    • , Feng Long
    • , William B. Whitman
    • , Pascal Lill
    • , Christos Gatsogiannis
    • , Stefan Raunser
    • , Nobutaka Shimizu
    • , Akira Shinoda
    • , Akiyoshi Nakamura
    • , Isao Tanaka
    •  & Min Yao

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