Cryoelectron tomography

Definition

Cryo-electron tomography (also cryo-ET or electron cryotomography) is a technique where an electron microscope is used to record a series of two-dimensional images as a biological sample held at cryogenic temperatures is tilted. Using computational methods, the two-dimensional images can be aligned to yield a three-dimensional (tomographic) reconstruction of the sample.

Latest Research and Reviews

News and Comment

  • Comments and Opinion | | open

    We report the outcomes of the discussion initiated at the workshop entitled A 3D Cellular Context for the Macromolecular World and propose how data from emerging three-dimensional (3D) cellular imaging techniques—such as electron tomography, 3D scanning electron microscopy and soft X-ray tomography—should be archived, curated, validated and disseminated, to enable their interpretation and reuse by the biomedical community.

    • Ardan Patwardhan
    • , Alun Ashton
    • , Robert Brandt
    • , Sarah Butcher
    • , Raffaella Carzaniga
    • , Wah Chiu
    • , Lucy Collinson
    • , Pascal Doux
    • , Elizabeth Duke
    • , Mark H Ellisman
    • , Erik Franken
    • , Kay Grünewald
    • , Jean-Karim Heriche
    • , Abraham Koster
    • , Werner Kühlbrandt
    • , Ingvar Lagerstedt
    • , Carolyn Larabell
    • , Catherine L Lawson
    • , Helen R Saibil
    • , Eduardo Sanz-García
    • , Sriram Subramaniam
    • , Paul Verkade
    • , Jason R Swedlow
    •  & Gerard J Kleywegt
  • Research Highlights |

    A milling technique affords researchers a high-resolution glimpse deep into the cell using cryoelectron tomography.

    • Allison Doerr