CRISPR-Cas systems

CRISPR-Cas systems are prokaryotic immune systems that confer resistance to foreign genetic elements such as plasmids and phages. CRISPR-Cas systems have been exploited for targeted genome editing.

Featured

Latest Research and Reviews

  • Research |

    Genome-edited human pluripotent stem cells and genome-edited mouse models reveal that combinatorial genetic interactions contribute to the complex genetic heritability of human cardiomyopathy.

    • Dekker C. Deacon
    • , Cassandra L. Happe
    • , Chao Chen
    • , Neil Tedeschi
    • , Ana Maria Manso
    • , Ting Li
    • , Nancy D. Dalton
    • , Qian Peng
    • , Elie N. Farah
    • , Yusu Gu
    • , Kevin P. Tenerelli
    • , Vivien D. Tran
    • , Ju Chen
    • , Kirk L. Peterson
    • , Nicholas J. Schork
    • , Eric D. Adler
    • , Adam J. Engler
    • , Robert S. Ross
    •  & Neil C. Chi
  • Research | | open

    The genomic locations that can be targeted for editing by CRISPR are limited by the presence of the nuclease-specific PAM sequence. Here, the authors show PAM recognition can be expanded by replacing the key region in the PAM interaction domain of SaCas9 with the corresponding region of SaCas9 orthologs.

    • Dacheng Ma
    • , Zhimeng Xu
    • , Zhaoyu Zhang
    • , Xi Chen
    • , Xiangzhi Zeng
    • , Yiyang Zhang
    • , Tingyue Deng
    • , Mengfei Ren
    • , Zheng Sun
    • , Rui Jiang
    •  & Zhen Xie
  • Research | | open

    Jaindra Tirpathi et al. report a strategy for inactivating endogenous banana streak virus sequences in the plantain B genome using CRISPR/Cas9. They show that three-quarters of edited plants had no viral symptoms under stress conditions, providing an improved B genome germplasm for plantain and banana breeding.

    • Jaindra N. Tripathi
    • , Valentine O. Ntui
    • , Mily Ron
    • , Samwel K. Muiruri
    • , Anne Britt
    •  & Leena Tripathi
  • Research | | open

    Multiple morphological abnormalities of the sperm flagella (MMAF) is a cause of male infertility. Here the authors identify homozygous nonsense mutations of the glutamine rich 2 (QRICH2) gene in two MMAF patients from 2 consanguineous families and show using QRICH2 knockout mice that the protein is required for sperm flagellar formation and motility.

    • Ying Shen
    • , Feng Zhang
    • , Fuping Li
    • , Xiaohui Jiang
    • , Yihong Yang
    • , Xiaoliang Li
    • , Weiyu Li
    • , Xiang Wang
    • , Juan Cheng
    • , Mohan Liu
    • , Xueguang Zhang
    • , Guiping Yuan
    • , Xue Pei
    • , Kailai Cai
    • , Fengyun Hu
    • , Jianfeng Sun
    • , Lanzhen Yan
    • , Li Tang
    • , Chuan Jiang
    • , Wenling Tu
    • , Jinyan Xu
    • , Haojuan Wu
    • , Weiqi Kong
    • , Shuying Li
    • , Ke Wang
    • , Kai Sheng
    • , Xudong Zhao
    • , Huanxun Yue
    • , Xiaoyu Yang
    •  & Wenming Xu

News and Comment