CRISPR-Cas systems

Definition

CRISPR-Cas systems are prokaryotic immune systems that confer resistance to foreign genetic elements such as plasmids and phages. CRISPR-Cas systems have been exploited for targeted genome editing.

Latest Research and Reviews

  • Research | | open

    RNA regulatory elements (RREs) are important post-transcriptional control features but studying them requires disrupting their activity without disturbing cellular homeostasis. Here the authors present GenERA, a CRISPR-Cas9 screening platform of in situ analysis of native RREs.

    • Qianxin Wu
    • , Quentin R. V. Ferry
    • , Toni A. Baeumler
    • , Yale S. Michaels
    • , Dimitrios M. Vitsios
    • , Omer Habib
    • , Roland Arnold
    • , Xiaowei Jiang
    • , Stefano Maio
    • , Bruno R. Steinkraus
    • , Marta Tapia
    • , Paolo Piazza
    • , Ni Xu
    • , Georg A. Holländer
    • , Thomas A. Milne
    • , Jin-Soo Kim
    • , Anton J. Enright
    • , Andrew R. Bassett
    •  & Tudor A. Fulga
  • Research | | open

    The double-strand breaks generated by CRISPR-Cas systems are the target of multiple DNA repair pathways. Here the authors find incompatibility between NHEJ and type II-A CRISPR-Cas systems due to Csn2 mediated inhibition of end-joining.

    • Aude Bernheim
    • , Alicia Calvo-Villamañán
    • , Clovis Basier
    • , Lun Cui
    • , Eduardo P. C. Rocha
    • , Marie Touchon
    •  & David Bikard
  • Research | | open

    Cpf1 is a promising addition to the CRISPR toolkit but displays wide variability of activity in different eurkaryotes. Here the authors identify temperature as a modulator of activity and use this to efficiently edit ectothermic vertebrate species.

    • Miguel A. Moreno-Mateos
    • , Juan P. Fernandez
    • , Romain Rouet
    • , Charles E. Vejnar
    • , Maura A. Lane
    • , Emily Mis
    • , Mustafa K. Khokha
    • , Jennifer A. Doudna
    •  & Antonio J. Giraldez
  • Reviews |

    The rapid development of CRISPR-based gene manipulation has enabled various approaches for high-throughput functional genomics. This Review guides users through the practicalities of CRISPR-based functional genomics screens, including study design options, best-practice approaches, pitfalls to avoid and data analysis strategies.

    • John G. Doench
  • Research | | open

    Cas9 and Cpf1 have both been adapted for genome engineering, editing and gene expression regulation. Here the authors design a fusion guide RNA that can interact with both proteins for multiple and orthogonal genome manipulation.

    • Jiyeon Kweon
    • , An-Hee Jang
    • , Da-Eun Kim
    • , Jin Wook Yang
    • , Mijung Yoon
    • , Ha Rim Shin
    • , Jin-Soo Kim
    •  & Yongsub Kim

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