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Chromatography is a technique used to separate mixtures of chemical compounds or biomolecules based on differences in their relative affinities for mobile (gas or liquid) and stationary (solid or liquid) phases. Chromatography is used for both preparative and analytical purposes and can be performed in column or planar format.
A two-laboratory study of the reproducibility of affinity purification–mass spectrometry shows that a standardized protocol results in highly reproducible interactome data.
