Chemical biology

  • Article
    | Open Access

    The Lys48-linked polyubiquitin-mediated proteasomal degradation in yeast depends on Cdc48 and its cofactors Ufd1 and Npl4. Here, the authors present crystal structures of Npl4 bound to Lys48-linked diubiquitin and the Npl4-binding motif of Ufd1, providing insights into the reaction mechanism of the Cdc48- Ufd1/Npl4 complex.

    • Yusuke Sato
    • , Hikaru Tsuchiya
    • , Atsushi Yamagata
    • , Kei Okatsu
    • , Keiji Tanaka
    • , Yasushi Saeki
    •  & Shuya Fukai
  • Article
    | Open Access

    The design of protein assemblies is a major thrust for biomolecular engineering and nanobiotechnology. Here the authors demonstrate a general mechanism for designing allosteric macromolecular assemblies and showcase a proof of concept for engineered allosteric protein assembly.

    • Luis A. Campos
    • , Rajendra Sharma
    • , Sara Alvira
    • , Federico M. Ruiz
    • , Beatriz Ibarra-Molero
    • , Mourad Sadqi
    • , Carlos Alfonso
    • , Germán Rivas
    • , Jose M. Sanchez-Ruiz
    • , Antonio Romero Garrido
    • , José M. Valpuesta
    •  & Victor Muñoz
  • Article
    | Open Access

    miRNA142-3p and Tet2 are separately known to regulate Treg. Here the authors show that miRNA142-3p targets Tet2 and by this opposes Treg differentiation in autoimmune diabetes.

    • Martin G. Scherm
    • , Isabelle Serr
    • , Adam M. Zahm
    • , Jonathan Schug
    • , Saverio Bellusci
    • , Rossella Manfredini
    • , Victoria K. Salb
    • , Katharina Gerlach
    • , Benno Weigmann
    • , Anette-Gabriele Ziegler
    • , Klaus H. Kaestner
    •  & Carolin Daniel
  • Article
    | Open Access

    Graft versus host disease (GvHD) still hinders allogeneic hematopoietic stem cell transplantation. Here, the authors use metabolomics to analyze two cohorts of paired transplant recipients and donors, identifying significant differences in both host- and microbiota-derived metabolites.

    • David Michonneau
    • , Eleonora Latis
    • , Emmanuel Curis
    • , Laetitia Dubouchet
    • , Sivapriya Ramamoorthy
    • , Brian Ingram
    • , Régis Peffault de Latour
    • , Marie Robin
    • , Flore Sicre de Fontbrune
    • , Sylvie Chevret
    • , Lars Rogge
    •  & Gérard Socié
  • Article
    | Open Access

    Elctrochemical water splitting is of vital significance for energy conversion and storage. Here the authors show an electrocatalyst based on amorphous ruthenium-tellurium porous nanorods which exhibit significantly improved OER performance than its crystalline counterparts

    • Juan Wang
    • , Lili Han
    • , Bolong Huang
    • , Qi Shao
    • , Huolin L. Xin
    •  & Xiaoqing Huang
  • Article
    | Open Access

    Cell senescence involves stable arrest of cell proliferation and changes in gene expression and 3D genome reorganization. Here, the authors show that human condensin II complex participates in reorganization of the chromatin compartments, primarily through switching from heterochromatic B to euchromatic A compartments.

    • Osamu Iwasaki
    • , Hideki Tanizawa
    • , Kyoung-Dong Kim
    • , Andrew Kossenkov
    • , Timothy Nacarelli
    • , Sanki Tashiro
    • , Sonali Majumdar
    • , Louise C. Showe
    • , Rugang Zhang
    •  & Ken-ichi Noma
  • Article
    | Open Access

    Single-molecule in vitro assays require dedicated confocal microscopes equipped with fluorescence correlation spectroscopy (FCS) modules. Here the authors present a compact, cheap and open-source 3D-printed confocal microscope for single photon counting and FCS measurements, and use it to detect α-synuclein aggregation.

    • James W. P. Brown
    • , Arnaud Bauer
    • , Mark E Polinkovsky
    • , Akshay Bhumkar
    • , Dominic J. B. Hunter
    • , Katharina Gaus
    • , Emma Sierecki
    •  & Yann Gambin
  • Article
    | Open Access

    XACT is a primate-specific TE-derived lncRNA that coats active X chromosomes in pluripotent cells and may contribute to species-specific regulation of X chromosome inactivation. Here, the authors investigate TEs associated with the XACT locus and identify a critical enhancer for its regulation, which evolved from an ancestral group of mammalian endogenous retroviruses, prior to the emergence of XACT.

    • Miguel Casanova
    • , Madeleine Moscatelli
    • , Louis Édouard Chauvière
    • , Christophe Huret
    • , Julia Samson
    • , Tharvesh Moideen Liyakat Ali
    • , Olga Rosspopoff
    •  & Claire Rougeulle
  • Article
    | Open Access

    While the cellular recycling process autophagy has been linked to aging, the impact of selective autophagy on lifespan remains unclear. Here Kumsta et al. show that the autophagy receptor p62/SQSTM1 is required for hormetic benefits and p62/SQSTM1 overexpression is sufficient to extend C. elegans lifespan and improve proteostasis.

    • Caroline Kumsta
    • , Jessica T. Chang
    • , Reina Lee
    • , Ee Phie Tan
    • , Yongzhi Yang
    • , Rute Loureiro
    • , Elizabeth H. Choy
    • , Shaun H. Y. Lim
    • , Isabel Saez
    • , Alexander Springhorn
    • , Thorsten Hoppe
    • , David Vilchez
    •  & Malene Hansen
  • Article
    | Open Access

    Multiple sequence alignments of proteins carry information about evolution, the protein’s fitness landscape and its stability in the face of mutations. Here, the authors demonstrate the utility of latent space models learned using variational autoencoders to infer these properties from sequences.

    • Xinqiang Ding
    • , Zhengting Zou
    •  & Charles L. Brooks III
  • Article
    | Open Access

    Plasmodium growth is adapted to the reproductive cycle of mosquitoes, but underlying mechanisms are unclear. Here, Lampe et al. show that the blood-meal induced miR-276 balances the termination of the mosquito amino acid catabolism and egg development, providing nutrients for Plasmodium sporozoite development.

    • Lena Lampe
    • , Marius Jentzsch
    • , Sylwia Kierszniowska
    •  & Elena A. Levashina
  • Article
    | Open Access

    Myeloid-derived growth factor (MYDGF) is an endoplasmic reticulum protein of therapeutic interest because it promotes tissue repair in a murine model of myocardial infarction. Here the authors present the NMR structure of human MYDGF and attribute function to a set of residues conserved in MYDGFs but not the vanin base domain, which has a similar fold.

    • Valeriu Bortnov
    • , Marco Tonelli
    • , Woonghee Lee
    • , Ziqing Lin
    • , Douglas S. Annis
    • , Omar N. Demerdash
    • , Alex Bateman
    • , Julie C. Mitchell
    • , Ying Ge
    • , John L. Markley
    •  & Deane F. Mosher
  • Article
    | Open Access

    Here, Bhattacharya et al. chemically mutagenize Leishmania and identify genes associated with resistance to miltefosine and paromomycin by next generation sequencing. The study shows that a protein kinase (CDPK1) can mediate resistance to paromomycin by affecting translation.

    • Arijit Bhattacharya
    • , Philippe Leprohon
    • , Sophia Bigot
    • , Prasad Kottayil Padmanabhan
    • , Angana Mukherjee
    • , Gaétan Roy
    • , Hélène Gingras
    • , Anais Mestdagh
    • , Barbara Papadopoulou
    •  & Marc Ouellette
  • Article
    | Open Access

    The mRNA-surveillance pathway of no-go decay (NGD) is a eukaryotic ribosome-based-quality-control process that targets transcripts that stall the ribosome. Here the authors show no-go decay (NGD) and ribosome-quality control (RQC) pathways are activated by mRNAs damaged by alkylation and oxidation stress.

    • Liewei L. Yan
    • , Carrie L. Simms
    • , Fionn McLoughlin
    • , Richard D. Vierstra
    •  & Hani S. Zaher
  • Article
    | Open Access

    Glycolysis is elevated in many cancers. In this study, the authors show that lactoylglutathione, a by-product of methylglyoxal produced from increased glycolysis, is elevated in lung cancer in mouse models and humans, arguing reactive metabolite production can be a liability for cancers.

    • Alba Luengo
    • , Keene L. Abbott
    • , Shawn M. Davidson
    • , Aaron M. Hosios
    • , Brandon Faubert
    • , Sze Ham Chan
    • , Elizaveta Freinkman
    • , Lauren G. Zacharias
    • , Thomas P. Mathews
    • , Clary B. Clish
    • , Ralph J. DeBerardinis
    • , Caroline A. Lewis
    •  & Matthew G. Vander Heiden
  • Article
    | Open Access

    Bacterial tRNA is modified by thiolation of nucleosides. Here the authors identify 2-methylthiocytidine in bacterial tRNA using nucleic acid isotope labeling coupled mass spectrometry. Exposure to methylating agents converts 2-thiocytidine to 2-methylthiocytidine, which is repaired by demethylase AlkB in vivo.

    • Valentin F. Reichle
    • , Dimitar P. Petrov
    • , Verena Weber
    • , Kirsten Jung
    •  & Stefanie Kellner
  • Article
    | Open Access

    Cysteinyl leukotriene G protein-coupled receptors CysLT1 and CysLT2 regulate pro-inflammatory responses associated with allergic disorders. Here, authors describe four crystal structures of CysLT2R in complex with three dual CysLT1R/CysLT2R antagonists, which shed light on CysLTR ligand selectivity.

    • Anastasiia Gusach
    • , Aleksandra Luginina
    • , Egor Marin
    • , Rebecca L. Brouillette
    • , Élie Besserer-Offroy
    • , Jean-Michel Longpré
    • , Andrii Ishchenko
    • , Petr Popov
    • , Nilkanth Patel
    • , Taku Fujimoto
    • , Toru Maruyama
    • , Benjamin Stauch
    • , Margarita Ergasheva
    • , Daria Romanovskaia
    • , Anastasiia Stepko
    • , Kirill Kovalev
    • , Mikhail Shevtsov
    • , Valentin Gordeliy
    • , Gye Won Han
    • , Vsevolod Katritch
    • , Valentin Borshchevskiy
    • , Philippe Sarret
    • , Alexey Mishin
    •  & Vadim Cherezov
  • Article
    | Open Access

    Trypanosoma brucei relies on uptake and conversion of purines from the host, which constitutes a potential drug target. Here, Hulpia et al. combine structural elements from known trypanocidal nucleoside analogues and develop a potent trypanocide with curative activity in animal models of acute and late stage sleeping sickness.

    • Fabian Hulpia
    • , Dorien Mabille
    • , Gustavo D. Campagnaro
    • , Gabriela Schumann
    • , Louis Maes
    • , Isabel Roditi
    • , Anders Hofer
    • , Harry P. de Koning
    • , Guy Caljon
    •  & Serge Van Calenbergh
  • Article
    | Open Access

    SPOR domains are present in bacterial proteins that recognize cell-wall peptidoglycan strands stripped of the peptide stems. Here, Alcorlo et al. show that, indeed, the presence of peptide stems abrogates binding to a SPOR domain, and provide insights into the molecular basis for recognition.

    • Martín Alcorlo
    • , David A. Dik
    • , Stefania De Benedetti
    • , Kiran V. Mahasenan
    • , Mijoon Lee
    • , Teresa Domínguez-Gil
    • , Dusan Hesek
    • , Elena Lastochkin
    • , Daniel López
    • , Bill Boggess
    • , Shahriar Mobashery
    •  & Juan A. Hermoso
  • Article
    | Open Access

    Tightly controlling cell output is challenging, which has limited development and applications of bacterial sensors. Here the authors develop tunable, fast-responding sensors to control production of metabolic pigments and use them to assess zinc deficiency in a low-cost, minimal equipment fashion.

    • Monica P. McNerney
    • , Cirstyn L. Michel
    • , Krishi Kishore
    • , Janet Standeven
    •  & Mark P. Styczynski
  • Article
    | Open Access

    DNA composite materials have potential for biomedical sciences; however, control over the materials can be an issue. Here, the authors report on a carbon-nanotube reinforced DNA-silica gel with controllable mechanical properties to steer the attachment, proliferation, migration and release of cells.

    • Yong Hu
    • , Carmen M. Domínguez
    • , Jens Bauer
    • , Simone Weigel
    • , Alessa Schipperges
    • , Claude Oelschlaeger
    • , Norbert Willenbacher
    • , Stephan Keppler
    • , Martin Bastmeyer
    • , Stefan Heißler
    • , Christof Wöll
    • , Tim Scharnweber
    • , Kersten S. Rabe
    •  & Christof M. Niemeyer
  • Article
    | Open Access

    CRISPR-Cas systems have well characterized, modular structures. Here the authors use that architecture to design a Cas12a library of 560 synthetic chimeras, with altered PAM preferences and specificities.

    • R. M. Liu
    • , L. L. Liang
    • , E. Freed
    • , H. Chang
    • , E. Oh
    • , Z. Y. Liu
    • , A. Garst
    • , C. A. Eckert
    •  & R. T. Gill
  • Article
    | Open Access

    The conformational heterogeneity of RNA molecules makes their structure determination by X-ray crystallography and NMR challenging. Here the authors show that RNA structures can be solved by cryo-EM and present the structures of a 40 kDa SAM-IV riboswitch in the apo form and bound to its ligand S-adenosylmethionine.

    • Kaiming Zhang
    • , Shanshan Li
    • , Kalli Kappel
    • , Grigore Pintilie
    • , Zhaoming Su
    • , Tung-Chung Mou
    • , Michael F. Schmid
    • , Rhiju Das
    •  & Wah Chiu
  • Article
    | Open Access

    LRIG1 is a tumour suppressor in several cancers. Here, the authors show that androgen receptor directly transactivates LRIG1 which then antagonises ERBB signalling and c-Myc expression to inhibit prostate tumorigenesis.

    • Qiuhui Li
    • , Bigang Liu
    • , Hsueh-Ping Chao
    • , Yibing Ji
    • , Yue Lu
    • , Rashid Mehmood
    • , Collene Jeter
    • , Taiping Chen
    • , John R. Moore
    • , Wenqian Li
    • , Can Liu
    • , Kiera Rycaj
    • , Amanda Tracz
    • , Jason Kirk
    • , Tammy Calhoun-Davis
    • , Jie Xiong
    • , Qu Deng
    • , Jiaoti Huang
    • , Barbara A. Foster
    • , Abhiram Gokhale
    • , Xin Chen
    •  & Dean G. Tang
  • Article
    | Open Access

    Type VI secretion systems (T6SSs) are used by bacteria to inject toxic effector proteins into neighbouring cells. Here, Mariano et al. show that an antibacterial effector from Serratia marcescens forms cation-selective pores that lead to inner-membrane depolarisation and increased outer-membrane permeability.

    • Giuseppina Mariano
    • , Katharina Trunk
    • , David J. Williams
    • , Laura Monlezun
    • , Henrik Strahl
    • , Samantha J. Pitt
    •  & Sarah J. Coulthurst
  • Article
    | Open Access

    The activation mechanism of integrin αVβ8 differs from other integrins. Combining X-ray crystallography, hydrogen deuterium exchange mass spectrometry and mutation, the authors reveal structural features responsible for these differences and provide insights into how typical integrins are regulated.

    • Jianchuan Wang
    • , Yang Su
    • , Roxana E. Iacob
    • , John R. Engen
    •  & Timothy A. Springer
  • Article
    | Open Access

    Crosstalk between protein oxidation and other post-translational modifications remains unexplored. Here, the authors map the phosphoproteome, cysteine redox proteome and total proteome of adipocytes under acute oxidative stress and reveal crosstalk between cysteine oxidation and phosphorylation-based signalling.

    • Zhiduan Su
    • , James G. Burchfield
    • , Pengyi Yang
    • , Sean J. Humphrey
    • , Guang Yang
    • , Deanne Francis
    • , Sabina Yasmin
    • , Sung-Young Shin
    • , Dougall M. Norris
    • , Alison L. Kearney
    • , Miro A. Astore
    • , Jonathan Scavuzzo
    • , Kelsey H. Fisher-Wellman
    • , Qiao-Ping Wang
    • , Benjamin L. Parker
    • , G. Gregory Neely
    • , Fatemeh Vafaee
    • , Joyce Chiu
    • , Reichelle Yeo
    • , Philip J. Hogg
    • , Daniel J. Fazakerley
    • , Lan K. Nguyen
    • , Serdar Kuyucak
    •  & David E. James
  • Article
    | Open Access

    Many cancer cells have increased glucose consumption compared to normal cells, a feature that can be exploited therapeutically. Here, the authors carry out a chemical screen and identify compounds that selectively blocks glucose metabolism in non-small-cell lung cancer cell lines.

    • Chiara Ghezzi
    • , Alicia Wong
    • , Bao Ying Chen
    • , Bernard Ribalet
    • , Robert Damoiseaux
    •  & Peter M. Clark
  • Article
    | Open Access

    Liquid-liquid phase separation (LLPS) of intrinsically disordered proteins plays an important part in the formation of extracellular biological materials. Here, the authors show that repeats of the peptide motif GHGLY are necessary for the LLPS of pH-responsive histidine-rich squid beak proteins.

    • Bartosz Gabryelczyk
    • , Hao Cai
    • , Xiangyan Shi
    • , Yue Sun
    • , Piet J. M. Swinkels
    • , Stefan Salentinig
    • , Konstantin Pervushin
    •  & Ali Miserez
  • Article
    | Open Access

    Viral genomic DNA is often modified to evade the host bacterial restriction system. Here the authors identified 2′-deoxy-7-deazaguanine modifications on phage DNA by comparative genomics and experimental validation, showing their role in genome protection.

    • Geoffrey Hutinet
    • , Witold Kot
    • , Liang Cui
    • , Roman Hillebrand
    • , Seetharamsingh Balamkundu
    • , Shanmugavel Gnanakalai
    • , Ramesh Neelakandan
    • , Alexander B. Carstens
    • , Chuan Fa Lui
    • , Denise Tremblay
    • , Deborah Jacobs-Sera
    • , Mandana Sassanfar
    • , Yan-Jiun Lee
    • , Peter Weigele
    • , Sylvain Moineau
    • , Graham F. Hatfull
    • , Peter C. Dedon
    • , Lars H. Hansen
    •  & Valérie de Crécy-Lagard
  • Article
    | Open Access

    How genome stability is maintained at regions of active transcription is currently not entirely clear. Here, the authors reveal an association between base excision repair factors and transcription elongation to modulate DNA repair.

    • Nicola P. Montaldo
    • , Diana L. Bordin
    • , Alessandro Brambilla
    • , Marcel Rösinger
    • , Sarah L. Fordyce Martin
    • , Karine Øian Bjørås
    • , Stefano Bradamante
    • , Per Arne Aas
    • , Antonia Furrer
    • , Lene C. Olsen
    • , Nicolas Kunath
    • , Marit Otterlei
    • , Pål Sætrom
    • , Magnar Bjørås
    • , Leona D. Samson
    •  & Barbara van Loon
  • Article
    | Open Access

    DNA ligases catalyze the joining of DNA strands to complete DNA replication, recombination and repair transactions. Here the authors present X-ray structures and kinetic analyses of LIG1 complexes with undamaged and oxidatively damaged DNA that unveil determinants of LIG1 substrate recognition and enzymatic fidelity.

    • Percy P. Tumbale
    • , Thomas J. Jurkiw
    • , Matthew J. Schellenberg
    • , Amanda A. Riccio
    • , Patrick J O’Brien
    •  & R. Scott Williams
  • Article
    | Open Access

    Established bacterial glycoengineering platforms limit access to protein and glycan substrates. Here the authors design a cytoplasmic protein glycosylation system, Glycoli, to generate a variety of multivalent glycostructures.

    • Hanne L. P. Tytgat
    • , Chia-wei Lin
    • , Mikail D. Levasseur
    • , Markus B. Tomek
    • , Christoph Rutschmann
    • , Jacqueline Mock
    • , Nora Liebscher
    • , Naohiro Terasaka
    • , Yusuke Azuma
    • , Michael Wetter
    • , Martin F. Bachmann
    • , Donald Hilvert
    • , Markus Aebi
    •  & Timothy G. Keys
  • Article
    | Open Access

    Constructing biosynthetic pathways to study and engineer glycoprotein structures is difficult. Here, the authors use GlycoPRIME, a cell-free workflow for mixing-and-matching glycosylation enzymes, to evaluate 37 putative glycosylation pathways and discover routes to 18 new glycoprotein structures

    • Weston Kightlinger
    • , Katherine E. Duncker
    • , Ashvita Ramesh
    • , Ariel H. Thames
    • , Aravind Natarajan
    • , Jessica C. Stark
    • , Allen Yang
    • , Liang Lin
    • , Milan Mrksich
    • , Matthew P. DeLisa
    •  & Michael C. Jewett
  • Article
    | Open Access

    Cytotoxic T lymphocytes (CTLs) eliminate virus-infected and cancerous cells by secreting the pore-forming protein (perforin) and pro-apoptotic serine proteases (granzymes). Here authors show that two mechanisms protect the membranes of CTLs from disruption by perforin and granzymes.

    • Jesse A. Rudd-Schmidt
    • , Adrian W. Hodel
    • , Tahereh Noori
    • , Jamie A. Lopez
    • , Hyun-Jung Cho
    • , Sandra Verschoor
    • , Annette Ciccone
    • , Joseph A. Trapani
    • , Bart W. Hoogenboom
    •  & Ilia Voskoboinik
  • Article
    | Open Access

    The coordination of interactions between multiple regulatory elements and genes within a chromatin domain remains poorly understood. Here, the authors use a method to detect multi-way chromatin interactions in a mouse model in which the α-globin domain is extended to include several additional genes, finding that the promoters do not form mutually exclusive interactions with the enhancers, but all interact simultaneously in a single complex.

    • A. Marieke Oudelaar
    • , Caroline L. Harrold
    • , Lars L. P. Hanssen
    • , Jelena M. Telenius
    • , Douglas R. Higgs
    •  & Jim R. Hughes
  • Article
    | Open Access

    There is disproportionally high cancer prevalence in males. Here, the authors analyse the tumour suppressor p53 in sporadic cancers, highlighting a higher incidence of its mutation in males. Males are further disadvantaged by a failure to shield against the expression of damaged X-linked genes in p53-networks. These factors likely contribute to sex-disparity.

    • Sue Haupt
    • , Franco Caramia
    • , Alan Herschtal
    • , Thierry Soussi
    • , Guillermina Lozano
    • , Hu Chen
    • , Han Liang
    • , Terence P. Speed
    •  & Ygal Haupt
  • Article
    | Open Access

    ISG15 is a ubiquitin-like modifier that can be upregulated in response to bacterial infections. Here, the authors use proteomics to identify endogenous ISGylation substrates in the liver of Listeria monocytogenes infected mice and show that ISGylation alters basal and infection-induced autophagy.

    • Yifeng Zhang
    • , Fabien Thery
    • , Nicholas C. Wu
    • , Emma K. Luhmann
    • , Olivier Dussurget
    • , Mariko Foecke
    • , Clara Bredow
    • , Daniel Jiménez-Fernández
    • , Kevin Leandro
    • , Antje Beling
    • , Klaus-Peter Knobeloch
    • , Francis Impens
    • , Pascale Cossart
    •  & Lilliana Radoshevich
  • Article
    | Open Access

    Several cancers harbour mutant p53 and express higher levels of p53-derived peptide-MHCs. Here, the authors report affinity matured human antibody, P1C1TM, specific for the p53125-134 peptide in complex with the HLA-A24 class I MHC molecule and show its efficacy and specificity for mutant p53 expressing tumours.

    • Lionel Low
    • , Angeline Goh
    • , Joanna Koh
    • , Samantha Lim
    •  & Cheng-I Wang
  • Article
    | Open Access

    Several functions have been proposed for m6A in RNA metabolism, yet little is known regarding the specific functions of individual m6A readers. Here, the authors observe that the m6A reader YTHDC2 — which contains an RNA helicase domain — acts on the coding region to promotes mRNA translation by resolving secondary structures.

    • Yuanhui Mao
    • , Leiming Dong
    • , Xiao-Min Liu
    • , Jiayin Guo
    • , Honghui Ma
    • , Bin Shen
    •  & Shu-Bing Qian
  • Article
    | Open Access

    In Drosophila, dosage compensation involves a twofold transcriptional upregulation of the single male chromosome X. Here the authors show that global conformational differences are specifically present in the male X chromosome and detectable using Hi-C data, indicating that dosage compensation affects global chromosome structure.

    • Koustav Pal
    • , Mattia Forcato
    • , Daniel Jost
    • , Thomas Sexton
    • , Cédric Vaillant
    • , Elisa Salviato
    • , Emilia Maria Cristina Mazza
    • , Enrico Lugli
    • , Giacomo Cavalli
    •  & Francesco Ferrari
  • Article
    | Open Access

    The chromosomal kinase JIL-1 is responsible for interphase histone H3S10 phosphorylation and has been proposed to protect active chromatin from heterochromatinisation. Here, the authors show that JIL-1 is stabilized and anchored to active genes and telomeric transposons by JASPer, which binds to H3K36me3 nucleosomes via its PWWP domain.

    • Christian Albig
    • , Chao Wang
    • , Geoffrey P. Dann
    • , Felix Wojcik
    • , Tamás Schauer
    • , Silke Krause
    • , Sylvain Maenner
    • , Weili Cai
    • , Yeran Li
    • , Jack Girton
    • , Tom W. Muir
    • , Jørgen Johansen
    • , Kristen M. Johansen
    • , Peter B. Becker
    •  & Catherine Regnard
  • Article
    | Open Access

    RNA-interacting proteome can be identified by RNA affinity purification followed by mass spectrometry. Here the authors developed a different RNA-centric technology that combines high-throughput immunoprecipitation of RNA binding proteins and luciferase-based detection of their interaction with the RNA.

    • Antoine Graindorge
    • , Inês Pinheiro
    • , Anna Nawrocka
    • , Allison C. Mallory
    • , Peter Tsvetkov
    • , Noa Gil
    • , Carlo Carolis
    • , Frank Buchholz
    • , Igor Ulitsky
    • , Edith Heard
    • , Mikko Taipale
    •  & Alena Shkumatava
  • Article
    | Open Access

    The endoplasmic reticulum (ER) is an intracellular network characterized by highly dynamic behavior whose control mechanisms are unclear. Here, the authors show that the ER-membrane protein Reticulon (Rtnl1) can constrict ER bilayers and lead to ER fission.

    • Javier Espadas
    • , Diana Pendin
    • , Rebeca Bocanegra
    • , Artur Escalada
    • , Giulia Misticoni
    • , Tatiana Trevisan
    • , Ariana Velasco del Olmo
    • , Aldo Montagna
    • , Sergio Bova
    • , Borja Ibarra
    • , Peter I. Kuzmin
    • , Pavel V. Bashkirov
    • , Anna V. Shnyrova
    • , Vadim A. Frolov
    •  & Andrea Daga