Checkpoints

Definition

Checkpoints are transition points in the cell cycle that can be negatively regulated. Checkpoints ensure that the cell only divides if conditions are favourable, and that the events take place in the correct order, for example that mitosis does not happen until DNA replication is complete.

Latest Research and Reviews

  • Research | | open

    A G1 cell cycle regulatory kinase Cip1 has been identified in budding yeast but how this is regulated is unclear. Here the authors identify cell cycle (Mcm1) and stress-mediated (Msn 2/4) transcription factors as regulating Cip1, causing stress induced CDK inhibition and delay in cell cycle progression.

    • Ya-Lan Chang
    • , Shun-Fu Tseng
    • , Yu-Ching Huang
    • , Zih-Jie Shen
    • , Pang-Hung Hsu
    • , Meng-Hsun Hsieh
    • , Chia-Wei Yang
    • , Silvia Tognetti
    • , Berta Canal
    • , Laia Subirana
    • , Chien-Wei Wang
    • , Hsiao-Tan Chen
    • , Chi-Ying Lin
    • , Francesc Posas
    •  & Shu-Chun Teng
  • Research | | open

    The spindle assembly checkpoint ensures correct chromosome segregation and relies on kinetochore localization of the Bub1 and Mad1/Mad2 checkpoint proteins. Here the authors show that main function of Bub1 is to position Mad1 close to KNL1 MELT repeats in human cells.

    • Gang Zhang
    • , Thomas Kruse
    • , Blanca López-Méndez
    • , Kathrine Beck Sylvestersen
    • , Dimitriya H. Garvanska
    • , Simone Schopper
    • , Michael Lund Nielsen
    •  & Jakob Nilsson
  • Research | | open

    Precise chromosome segregation during mitosis requires coordination of stable chromosome bi-orientation with anaphase onset, however the underlying mechanism is not clear. Here the authors show that inner centromere localization of the chromosomal passenger complex maintains centromeric cohesion on bi-oriented chromosomes and allows mitotic checkpoint silencing.

    • Rutger C. C. Hengeveld
    • , Martijn J. M. Vromans
    • , Mathijs Vleugel
    • , Michael A. Hadders
    •  & Susanne M. A. Lens
  • Reviews |

    Proteins regulating cell cycle progression are involved in the formation of most cancer types. This Review discusses the role of cell cycle proteins in cancer, the rationale for targeting them in cancer treatment, results of clinical trials, as well as future therapeutic potential of various cell cycle inhibitors.

    • Tobias Otto
    •  & Piotr Sicinski
  • Research |

    The near-complete in vitro reconstitution of the mitotic spindle assembly checkpoint reveals how the assembly of its effector, the mitotic checkpoint complex, is catalysed.

    • Alex C. Faesen
    • , Maria Thanasoula
    • , Stefano Maffini
    • , Claudia Breit
    • , Franziska Müller
    • , Suzan van Gerwen
    • , Tanja Bange
    •  & Andrea Musacchio
    Nature 542, 498–502

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