Checkpoints

Definition

Checkpoints are transition points in the cell cycle that can be negatively regulated. Checkpoints ensure that the cell only divides if conditions are favourable, and that the events take place in the correct order, for example that mitosis does not happen until DNA replication is complete.

Latest Research and Reviews

  • Reviews |

    Proteins regulating cell cycle progression are involved in the formation of most cancer types. This Review discusses the role of cell cycle proteins in cancer, the rationale for targeting them in cancer treatment, results of clinical trials, as well as future therapeutic potential of various cell cycle inhibitors.

    • Tobias Otto
    •  & Piotr Sicinski
  • Research |

    The near-complete in vitro reconstitution of the mitotic spindle assembly checkpoint reveals how the assembly of its effector, the mitotic checkpoint complex, is catalysed.

    • Alex C. Faesen
    • , Maria Thanasoula
    • , Stefano Maffini
    • , Claudia Breit
    • , Franziska Müller
    • , Suzan van Gerwen
    • , Tanja Bange
    •  & Andrea Musacchio
    Nature 542, 498–502
  • Research | | open

    The mitotic exit network (MEN) triggers mitotic exit and can be blocked by the spindle position checkpoint (SPOC). Here the authors show that SPOC kinase Kin4 counterbalances MEN activation by the Cdc fourteen early anaphase release (FEAR) network in the mother cell and that in the absence of FEAR mitotic exit requires daughter cell-confined factors.

    • Ayse Koca Caydasi
    • , Anton Khmelinskii
    • , Rafael Duenas-Sanchez
    • , Bahtiyar Kurtulmus
    • , Michael Knop
    •  & Gislene Pereira
  • Research |

    A high-resolution structure of a complex between the anaphase-promoting complex (APC/C) and the mitotic checkpoint complex (MCC) reveals how MCC interacts with and represses APC/C by obstructing substrate recognition and suppressing E3 ligase activity.

    • Claudio Alfieri
    • , Leifu Chang
    • , Ziguo Zhang
    • , Jing Yang
    • , Sarah Maslen
    • , Mark Skehel
    •  & David Barford
    Nature 536, 431–436
  • Research | | open

    End-resection of double strand DNA breaks is essential for pathway choice between non-homologous end-joining and homologous recombination. Here the authors show that phosphorylation of WRN helicase by CDK1 is essential for resection at replication-related breaks.

    • Valentina Palermo
    • , Sara Rinalducci
    • , Massimo Sanchez
    • , Francesca Grillini
    • , Joshua A. Sommers
    • , Robert M. Brosh Jr
    • , Lello Zolla
    • , Annapaola Franchitto
    •  & Pietro Pichierri

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