Cellular imaging

Definition

Cellular imaging encompasses the techniques that allow the detection and analysis of cellular organelles and macromolecules. Cellular imaging observations are obtained using light-based or electron-based microscopes and often request further analysis with computer based programming.

Latest Research and Reviews

  • Research |

    Two studies by Sugden et al. and Jin et al. show that endoglin regulates endothelial cell migration through VEGFR2 signalling and controls blood vessel diameter in response to blood flow.

    • Wade W. Sugden
    • , Robert Meissner
    • , Tinri Aegerter-Wilmsen
    • , Roman Tsaryk
    • , Elvin V. Leonard
    • , Jeroen Bussmann
    • , Mailin J. Hamm
    • , Wiebke Herzog
    • , Yi Jin
    • , Lars Jakobsson
    • , Cornelia Denz
    •  & Arndt F. Siekmann
  • Protocols |

    In DNA-PAINT, transient binding of dye-labeled oligonucleotides to their target strands creates the ‘blinking’ required for stochastic nanoscopy. This protocol describes how to apply DNA-PAINT, from sample preparation to data processing.

    • Joerg Schnitzbauer
    • , Maximilian T Strauss
    • , Thomas Schlichthaerle
    • , Florian Schueder
    •  & Ralf Jungmann
    Nature Protocols 12, 1198–1228
  • Research |

    Cancer stem cells are typically refractory to conventional treatments. Now, an unprecedented mechanism has been discovered by which salinomycin and derivatives can sequester iron in lysosomes leading to cytoplasmic iron depletion and the subsequent production of reactive oxygen species that are lethal to the cell. This discovery of the importance of iron in cancer stem cell maintenance provides an opportunity for developing new therapeutics.

    • Trang Thi Mai
    • , Ahmed Hamaï
    • , Antje Hienzsch
    • , Tatiana Cañeque
    • , Sebastian Müller
    • , Julien Wicinski
    • , Olivier Cabaud
    • , Christine Leroy
    • , Amandine David
    • , Verónica Acevedo
    • , Akihide Ryo
    • , Christophe Ginestier
    • , Daniel Birnbaum
    • , Emmanuelle Charafe-Jauffret
    • , Patrice Codogno
    • , Maryam Mehrpour
    •  & Raphaël Rodriguez
  • Research | | open

    The mechanisms linking phenotypic heterogeneity to collective cancer invasion are unclear. Here the authors develop an image-guided genomic technique to select and amplify leader and follower cells from in vitro invading cell packs and find a cooperative symbiotic relationship between these two cell populations.

    • J. Konen
    • , E. Summerbell
    • , B. Dwivedi
    • , K. Galior
    • , Y. Hou
    • , L. Rusnak
    • , A. Chen
    • , J. Saltz
    • , W. Zhou
    • , L. H. Boise
    • , P. Vertino
    • , L. Cooper
    • , K. Salaita
    • , J. Kowalski
    •  & A. I. Marcus

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