Biological techniques

Definition

Biological techniques are methods or procedures that are used to study living things. They include experimental and computational methods, approaches, protocols and tools for biological research.

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Latest Research and Reviews

  • Protocols |

    The levels of monoamines and their cofactors in cerebrospinal fluid are strong indicators for dopamine and serotonin biosynthesis and turnover. This protocol describes a set of HPLC-based approaches for the quantitative detection of these molecules.

    • Marta Batllori
    • , Marta Molero-Luis
    • , Aida Ormazabal
    • , Mercedes Casado
    • , Cristina Sierra
    • , Angels García-Cazorla
    • , Manju Kurian
    • , Simon Pope
    • , Simon J Heales
    •  & Rafael Artuch
    Nature Protocols 12, 2359–2375
  • Research |

    Small molecules are identified that inhibit the ubiquitin-specific protease USP7 with high affinity and specificity as explained by co-crystal structures, and are shown to reduce tumour growth in mice.

    • Andrew P. Turnbull
    • , Stephanos Ioannidis
    • , Wojciech W. Krajewski
    • , Adan Pinto-Fernandez
    • , Claire Heride
    • , Agnes C. L. Martin
    • , Louise M. Tonkin
    • , Elizabeth C. Townsend
    • , Shane M. Buker
    • , David R. Lancia
    • , Justin A. Caravella
    • , Angela V. Toms
    • , Thomas M. Charlton
    • , Johanna Lahdenranta
    • , Erik Wilker
    • , Bruce C. Follows
    • , Nicola J. Evans
    • , Lucy Stead
    • , Cristina Alli
    • , Vladislav V. Zarayskiy
    • , Adam C. Talbot
    • , Alexandre J. Buckmelter
    • , Minghua Wang
    • , Crystal L. McKinnon
    • , Fabienne Saab
    • , Joanna F. McGouran
    • , Hannah Century
    • , Malte Gersch
    • , Marc S. Pittman
    • , C. Gary Marshall
    • , Tony M. Raynham
    • , Mary Simcox
    • , Lorna M. D. Stewart
    • , Sheila B. McLoughlin
    • , Jaime A. Escobedo
    • , Kenneth W. Bair
    • , Christopher J. Dinsmore
    • , Tim R. Hammonds
    • , Sunkyu Kim
    • , Sylvie Urbé
    • , Michael J. Clague
    • , Benedikt M. Kessler
    •  & David Komander
  • Research |

    Single-particle cryo-electron microscopy is used to resolve the structure of the phycobilisome, a 16.8-megadalton light-harvesting megacomplex, from the red alga Griffithsia pacifica at a resolution of 3.5 Å.

    • Jun Zhang
    • , Jianfei Ma
    • , Desheng Liu
    • , Song Qin
    • , Shan Sun
    • , Jindong Zhao
    •  & Sen-Fang Sui
  • Research | | open

    Copper-dependent lytic polysaccharide monooxygenases (LPMOs) oxidatively cleave polysaccharides. Here the authors present a structure-function characterization of fungal LPMOs, showing that a particular LPMO cleaves xylan using a mechanism that involves an alternative copper coordination geometry.

    • T. J. Simmons
    • , K. E. H. Frandsen
    • , L. Ciano
    • , T. Tryfona
    • , N. Lenfant
    • , J. C. Poulsen
    • , L. F. L. Wilson
    • , T. Tandrup
    • , M. Tovborg
    • , K. Schnorr
    • , K. S. Johansen
    • , B. Henrissat
    • , P. H. Walton
    • , L Lo Leggio
    •  & P. Dupree

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