Biolistics articles from across Nature Portfolio

Latest Research and Reviews

• 

  Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells

  Ballistic delivery with micro/nano-particles has been successfully used to transfect plant cells, however, has failed in mammalian cells due to toxic effects. Here, the authors report on a self-assembled nano-ballistic delivery system for the delivery of functional macromolecules and demonstrate efficient transfection of mammalian cells.

  • Juan C. Fraire
  • Elnaz Shaabani
  • Kevin Braeckmans

  ResearchOpen Access14 Apr 2022 Nature Communications

  Volume: 13, P: 1996
• 

  Complex consisting of antisense DNA and β-glucan promotes internalization into cell through Dectin-1 and hybridizes with target mRNA in cytosol

  • Nobuaki Fujiwara
  • Hiroto Izumi
  • Shinichi Mochizuki

  Research04 Jul 2018 Cancer Gene Therapy

  Volume: 26, P: 32-40
• 

  Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 in vitro transcripts or ribonucleoproteins

  This protocol extension describes DNA-free genome editing of bread wheat by delivering in vitro transcripts (IVTs) or ribonucleoprotein complexes (RNPs) of CRISPR/Cas9 by particle bombardment. The authors' previously published protocol for genome editing of wheat used CRISPR/Cas9 plasmids.

  • Zhen Liang
  • Kunling Chen
  • Caixia Gao

  Protocols01 Feb 2018 Nature Protocols

  Volume: 13, P: 413-430
• 

  Transient expression and analysis of fluorescent reporter proteins in plant pollen tubes

  • Hao Wang
  • Liwen Jiang

  Protocols10 Mar 2011 Nature Protocols

  Volume: 6, P: 419-426