Biochemistry

  • Article
    | Open Access

    The Lys48-linked polyubiquitin-mediated proteasomal degradation in yeast depends on Cdc48 and its cofactors Ufd1 and Npl4. Here, the authors present crystal structures of Npl4 bound to Lys48-linked diubiquitin and the Npl4-binding motif of Ufd1, providing insights into the reaction mechanism of the Cdc48- Ufd1/Npl4 complex.

    • Yusuke Sato
    • , Hikaru Tsuchiya
    • , Atsushi Yamagata
    • , Kei Okatsu
    • , Keiji Tanaka
    • , Yasushi Saeki
    •  & Shuya Fukai
  • Article
    | Open Access

    The design of protein assemblies is a major thrust for biomolecular engineering and nanobiotechnology. Here the authors demonstrate a general mechanism for designing allosteric macromolecular assemblies and showcase a proof of concept for engineered allosteric protein assembly.

    • Luis A. Campos
    • , Rajendra Sharma
    • , Sara Alvira
    • , Federico M. Ruiz
    • , Beatriz Ibarra-Molero
    • , Mourad Sadqi
    • , Carlos Alfonso
    • , Germán Rivas
    • , Jose M. Sanchez-Ruiz
    • , Antonio Romero Garrido
    • , José M. Valpuesta
    •  & Victor Muñoz
  • Article
    | Open Access

    miRNA142-3p and Tet2 are separately known to regulate Treg. Here the authors show that miRNA142-3p targets Tet2 and by this opposes Treg differentiation in autoimmune diabetes.

    • Martin G. Scherm
    • , Isabelle Serr
    • , Adam M. Zahm
    • , Jonathan Schug
    • , Saverio Bellusci
    • , Rossella Manfredini
    • , Victoria K. Salb
    • , Katharina Gerlach
    • , Benno Weigmann
    • , Anette-Gabriele Ziegler
    • , Klaus H. Kaestner
    •  & Carolin Daniel
  • Article
    | Open Access

    Graft versus host disease (GvHD) still hinders allogeneic hematopoietic stem cell transplantation. Here, the authors use metabolomics to analyze two cohorts of paired transplant recipients and donors, identifying significant differences in both host- and microbiota-derived metabolites.

    • David Michonneau
    • , Eleonora Latis
    • , Emmanuel Curis
    • , Laetitia Dubouchet
    • , Sivapriya Ramamoorthy
    • , Brian Ingram
    • , Régis Peffault de Latour
    • , Marie Robin
    • , Flore Sicre de Fontbrune
    • , Sylvie Chevret
    • , Lars Rogge
    •  & Gérard Socié
  • Article
    | Open Access

    Cell senescence involves stable arrest of cell proliferation and changes in gene expression and 3D genome reorganization. Here, the authors show that human condensin II complex participates in reorganization of the chromatin compartments, primarily through switching from heterochromatic B to euchromatic A compartments.

    • Osamu Iwasaki
    • , Hideki Tanizawa
    • , Kyoung-Dong Kim
    • , Andrew Kossenkov
    • , Timothy Nacarelli
    • , Sanki Tashiro
    • , Sonali Majumdar
    • , Louise C. Showe
    • , Rugang Zhang
    •  & Ken-ichi Noma
  • Article
    | Open Access

    UVRAG is involved in autophagy, which loses its tumour suppressor functions when in its truncated form in cancers. Here, the authors use a mouse model that inducibly express this truncated protein and show impaired autophagy, enhanced inflammation and β-catenin stabilisation, which promotes spontaneous tumorigenesis.

    • Christine Quach
    • , Ying Song
    • , Hongrui Guo
    • , Shun Li
    • , Hadi Maazi
    • , Marshall Fung
    • , Nathaniel Sands
    • , Douglas O’Connell
    • , Sara Restrepo-Vassalli
    • , Billy Chai
    • , Dali Nemecio
    • , Vasu Punj
    • , Omid Akbari
    • , Gregory E. Idos
    • , Shannon M. Mumenthaler
    • , Nancy Wu
    • , Sue Ellen Martin
    • , Ashley Hagiya
    • , James Hicks
    • , Hengmin Cui
    •  & Chengyu Liang
  • Article
    | Open Access

    Single-molecule in vitro assays require dedicated confocal microscopes equipped with fluorescence correlation spectroscopy (FCS) modules. Here the authors present a compact, cheap and open-source 3D-printed confocal microscope for single photon counting and FCS measurements, and use it to detect α-synuclein aggregation.

    • James W. P. Brown
    • , Arnaud Bauer
    • , Mark E Polinkovsky
    • , Akshay Bhumkar
    • , Dominic J. B. Hunter
    • , Katharina Gaus
    • , Emma Sierecki
    •  & Yann Gambin
  • Article
    | Open Access

    XACT is a primate-specific TE-derived lncRNA that coats active X chromosomes in pluripotent cells and may contribute to species-specific regulation of X chromosome inactivation. Here, the authors investigate TEs associated with the XACT locus and identify a critical enhancer for its regulation, which evolved from an ancestral group of mammalian endogenous retroviruses, prior to the emergence of XACT.

    • Miguel Casanova
    • , Madeleine Moscatelli
    • , Louis Édouard Chauvière
    • , Christophe Huret
    • , Julia Samson
    • , Tharvesh Moideen Liyakat Ali
    • , Olga Rosspopoff
    •  & Claire Rougeulle
  • Article
    | Open Access

    Earth’s carbon cycle and oceanic magnesium cycle are controlled by processes such as weathering, volcanism and precipitation of carbonates, such as dolomite. Here, the authors contradict the view that modern dolomite formation is rare and suggest instead that dolomite accounts for ~40–60% of the global oceanic Mg output in the last 20 Ma.

    • Netta Shalev
    • , Tomaso R. R. Bontognali
    • , C. Geoffrey Wheat
    •  & Derek Vance
  • Article
    | Open Access

    While the cellular recycling process autophagy has been linked to aging, the impact of selective autophagy on lifespan remains unclear. Here Kumsta et al. show that the autophagy receptor p62/SQSTM1 is required for hormetic benefits and p62/SQSTM1 overexpression is sufficient to extend C. elegans lifespan and improve proteostasis.

    • Caroline Kumsta
    • , Jessica T. Chang
    • , Reina Lee
    • , Ee Phie Tan
    • , Yongzhi Yang
    • , Rute Loureiro
    • , Elizabeth H. Choy
    • , Shaun H. Y. Lim
    • , Isabel Saez
    • , Alexander Springhorn
    • , Thorsten Hoppe
    • , David Vilchez
    •  & Malene Hansen
  • Article
    | Open Access

    It was known that prolonged TRMPL1 activation induces TFEB translocation and upregulates autophagic gene regulation. Here, the authors show that acute TRMPL1 activation also induces autophagy through VPS34 and by lysosomal calcium release independent of TFEB.

    • A. Scotto Rosato
    • , S. Montefusco
    • , C. Soldati
    • , S. Di Paola
    • , A. Capuozzo
    • , J. Monfregola
    • , E. Polishchuk
    • , A. Amabile
    • , C. Grimm
    • , A. Lombardo
    • , M. A. De Matteis
    • , A. Ballabio
    •  & D. L. Medina
  • Article
    | Open Access

    Plasmodium growth is adapted to the reproductive cycle of mosquitoes, but underlying mechanisms are unclear. Here, Lampe et al. show that the blood-meal induced miR-276 balances the termination of the mosquito amino acid catabolism and egg development, providing nutrients for Plasmodium sporozoite development.

    • Lena Lampe
    • , Marius Jentzsch
    • , Sylwia Kierszniowska
    •  & Elena A. Levashina
  • Article
    | Open Access

    The contribution of symbiotic dinitrogen fixation to the forest carbon sink could change throughout forest succession. Here the authors model nitrogen cycling and light competition between trees based on data from Panamanian forest plots, showing that fixation contributes substantially to the carbon sink in early successional stages.

    • Jennifer H. Levy-Varon
    • , Sarah A. Batterman
    • , David Medvigy
    • , Xiangtao Xu
    • , Jefferson S. Hall
    • , Michiel van Breugel
    •  & Lars O. Hedin
  • Article
    | Open Access

    Myeloid-derived growth factor (MYDGF) is an endoplasmic reticulum protein of therapeutic interest because it promotes tissue repair in a murine model of myocardial infarction. Here the authors present the NMR structure of human MYDGF and attribute function to a set of residues conserved in MYDGFs but not the vanin base domain, which has a similar fold.

    • Valeriu Bortnov
    • , Marco Tonelli
    • , Woonghee Lee
    • , Ziqing Lin
    • , Douglas S. Annis
    • , Omar N. Demerdash
    • , Alex Bateman
    • , Julie C. Mitchell
    • , Ying Ge
    • , John L. Markley
    •  & Deane F. Mosher
  • Article
    | Open Access

    Here, Bhattacharya et al. chemically mutagenize Leishmania and identify genes associated with resistance to miltefosine and paromomycin by next generation sequencing. The study shows that a protein kinase (CDPK1) can mediate resistance to paromomycin by affecting translation.

    • Arijit Bhattacharya
    • , Philippe Leprohon
    • , Sophia Bigot
    • , Prasad Kottayil Padmanabhan
    • , Angana Mukherjee
    • , Gaétan Roy
    • , Hélène Gingras
    • , Anais Mestdagh
    • , Barbara Papadopoulou
    •  & Marc Ouellette
  • Article
    | Open Access

    The mRNA-surveillance pathway of no-go decay (NGD) is a eukaryotic ribosome-based-quality-control process that targets transcripts that stall the ribosome. Here the authors show no-go decay (NGD) and ribosome-quality control (RQC) pathways are activated by mRNAs damaged by alkylation and oxidation stress.

    • Liewei L. Yan
    • , Carrie L. Simms
    • , Fionn McLoughlin
    • , Richard D. Vierstra
    •  & Hani S. Zaher
  • Article
    | Open Access

    Glycolysis is elevated in many cancers. In this study, the authors show that lactoylglutathione, a by-product of methylglyoxal produced from increased glycolysis, is elevated in lung cancer in mouse models and humans, arguing reactive metabolite production can be a liability for cancers.

    • Alba Luengo
    • , Keene L. Abbott
    • , Shawn M. Davidson
    • , Aaron M. Hosios
    • , Brandon Faubert
    • , Sze Ham Chan
    • , Elizaveta Freinkman
    • , Lauren G. Zacharias
    • , Thomas P. Mathews
    • , Clary B. Clish
    • , Ralph J. DeBerardinis
    • , Caroline A. Lewis
    •  & Matthew G. Vander Heiden
  • Article
    | Open Access

    Bacterial tRNA is modified by thiolation of nucleosides. Here the authors identify 2-methylthiocytidine in bacterial tRNA using nucleic acid isotope labeling coupled mass spectrometry. Exposure to methylating agents converts 2-thiocytidine to 2-methylthiocytidine, which is repaired by demethylase AlkB in vivo.

    • Valentin F. Reichle
    • , Dimitar P. Petrov
    • , Verena Weber
    • , Kirsten Jung
    •  & Stefanie Kellner
  • Article
    | Open Access

    Cysteinyl leukotriene G protein-coupled receptors CysLT1 and CysLT2 regulate pro-inflammatory responses associated with allergic disorders. Here, authors describe four crystal structures of CysLT2R in complex with three dual CysLT1R/CysLT2R antagonists, which shed light on CysLTR ligand selectivity.

    • Anastasiia Gusach
    • , Aleksandra Luginina
    • , Egor Marin
    • , Rebecca L. Brouillette
    • , Élie Besserer-Offroy
    • , Jean-Michel Longpré
    • , Andrii Ishchenko
    • , Petr Popov
    • , Nilkanth Patel
    • , Taku Fujimoto
    • , Toru Maruyama
    • , Benjamin Stauch
    • , Margarita Ergasheva
    • , Daria Romanovskaia
    • , Anastasiia Stepko
    • , Kirill Kovalev
    • , Mikhail Shevtsov
    • , Valentin Gordeliy
    • , Gye Won Han
    • , Vsevolod Katritch
    • , Valentin Borshchevskiy
    • , Philippe Sarret
    • , Alexey Mishin
    •  & Vadim Cherezov
  • Article
    | Open Access

    SPOR domains are present in bacterial proteins that recognize cell-wall peptidoglycan strands stripped of the peptide stems. Here, Alcorlo et al. show that, indeed, the presence of peptide stems abrogates binding to a SPOR domain, and provide insights into the molecular basis for recognition.

    • Martín Alcorlo
    • , David A. Dik
    • , Stefania De Benedetti
    • , Kiran V. Mahasenan
    • , Mijoon Lee
    • , Teresa Domínguez-Gil
    • , Dusan Hesek
    • , Elena Lastochkin
    • , Daniel López
    • , Bill Boggess
    • , Shahriar Mobashery
    •  & Juan A. Hermoso
  • Article
    | Open Access

    MLL family histone methyltransferases deposit histone H3 Lys4 mono-/di-/tri-methylation and regulate gene expression in mammals. Here the authors report the single-particle cryo-EM structure of the NCP-bound human MLL1 core complex, shedding light on how the MLL1 complex engages chromatin and how chromatin binding promotes MLL1 tri-methylation activity.

    • Sang Ho Park
    • , Alex Ayoub
    • , Young-Tae Lee
    • , Jing Xu
    • , Hanseong Kim
    • , Wei Zheng
    • , Biao Zhang
    • , Liang Sha
    • , Sojin An
    • , Yang Zhang
    • , Michael A. Cianfrocco
    • , Min Su
    • , Yali Dou
    •  & Uhn-Soo Cho
  • Article
    | Open Access

    RNA polymerase I (Pol I) catalyses the transcription of pre-ribosomal RNA and for transcription initiation Pol I assembles with core factor and Rrn3 on the rDNA core promoter. Here the authors provide insights into the molecular mechanism of promoter opening by Pol I by determining the cryo-EM structures of two closed, two intermediate and two open Pol I pre-initiation complexes.

    • Yashar Sadian
    • , Florence Baudin
    • , Lucas Tafur
    • , Brice Murciano
    • , Rene Wetzel
    • , Felix Weis
    •  & Christoph W. Müller
  • Article
    | Open Access

    DNA composite materials have potential for biomedical sciences; however, control over the materials can be an issue. Here, the authors report on a carbon-nanotube reinforced DNA-silica gel with controllable mechanical properties to steer the attachment, proliferation, migration and release of cells.

    • Yong Hu
    • , Carmen M. Domínguez
    • , Jens Bauer
    • , Simone Weigel
    • , Alessa Schipperges
    • , Claude Oelschlaeger
    • , Norbert Willenbacher
    • , Stephan Keppler
    • , Martin Bastmeyer
    • , Stefan Heißler
    • , Christof Wöll
    • , Tim Scharnweber
    • , Kersten S. Rabe
    •  & Christof M. Niemeyer
  • Article
    | Open Access

    Help from CD4+ T cells is important to induce CD8+ T cell memory responses, but mechanistic insights are lacking. Here, the authors show, by transcriptomics and epigenetics, how CD4+ T cells help program memory CD8+ T cells for help-independent recall by antigens, as well as for innate-like recall responses by IL-12/IL-18 and promoting survival by IL-15.

    • Tomasz Ahrends
    • , Julia Busselaar
    • , Tesa M. Severson
    • , Nikolina Bąbała
    • , Evert de Vries
    • , Astrid Bovens
    • , Lodewyk Wessels
    • , Fred van Leeuwen
    •  & Jannie Borst
  • Article
    | Open Access

    Parkinson’s disease (PD) and Multiple System Atrophy (MSA) are characterized by the pathological accumulation of α-synuclein. Here the authors employ fluorescent probes, electron microscopy and NMR spectroscopy to study the properties of α-synuclein aggregates that were amplified from patient brain extracts and observe a greater structural diversity among PD patients compared to MSA patients.

    • Timo Strohäker
    • , Byung Chul Jung
    • , Shu-Hao Liou
    • , Claudio O. Fernandez
    • , Dietmar Riedel
    • , Stefan Becker
    • , Glenda M. Halliday
    • , Marina Bennati
    • , Woojin S. Kim
    • , Seung-Jae Lee
    •  & Markus Zweckstetter
  • Article
    | Open Access

    The conformational heterogeneity of RNA molecules makes their structure determination by X-ray crystallography and NMR challenging. Here the authors show that RNA structures can be solved by cryo-EM and present the structures of a 40 kDa SAM-IV riboswitch in the apo form and bound to its ligand S-adenosylmethionine.

    • Kaiming Zhang
    • , Shanshan Li
    • , Kalli Kappel
    • , Grigore Pintilie
    • , Zhaoming Su
    • , Tung-Chung Mou
    • , Michael F. Schmid
    • , Rhiju Das
    •  & Wah Chiu
  • Article
    | Open Access

    LRIG1 is a tumour suppressor in several cancers. Here, the authors show that androgen receptor directly transactivates LRIG1 which then antagonises ERBB signalling and c-Myc expression to inhibit prostate tumorigenesis.

    • Qiuhui Li
    • , Bigang Liu
    • , Hsueh-Ping Chao
    • , Yibing Ji
    • , Yue Lu
    • , Rashid Mehmood
    • , Collene Jeter
    • , Taiping Chen
    • , John R. Moore
    • , Wenqian Li
    • , Can Liu
    • , Kiera Rycaj
    • , Amanda Tracz
    • , Jason Kirk
    • , Tammy Calhoun-Davis
    • , Jie Xiong
    • , Qu Deng
    • , Jiaoti Huang
    • , Barbara A. Foster
    • , Abhiram Gokhale
    • , Xin Chen
    •  & Dean G. Tang
  • Article
    | Open Access

    Type VI secretion systems (T6SSs) are used by bacteria to inject toxic effector proteins into neighbouring cells. Here, Mariano et al. show that an antibacterial effector from Serratia marcescens forms cation-selective pores that lead to inner-membrane depolarisation and increased outer-membrane permeability.

    • Giuseppina Mariano
    • , Katharina Trunk
    • , David J. Williams
    • , Laura Monlezun
    • , Henrik Strahl
    • , Samantha J. Pitt
    •  & Sarah J. Coulthurst
  • Article
    | Open Access

    The activation mechanism of integrin αVβ8 differs from other integrins. Combining X-ray crystallography, hydrogen deuterium exchange mass spectrometry and mutation, the authors reveal structural features responsible for these differences and provide insights into how typical integrins are regulated.

    • Jianchuan Wang
    • , Yang Su
    • , Roxana E. Iacob
    • , John R. Engen
    •  & Timothy A. Springer
  • Article
    | Open Access

    Crosstalk between protein oxidation and other post-translational modifications remains unexplored. Here, the authors map the phosphoproteome, cysteine redox proteome and total proteome of adipocytes under acute oxidative stress and reveal crosstalk between cysteine oxidation and phosphorylation-based signalling.

    • Zhiduan Su
    • , James G. Burchfield
    • , Pengyi Yang
    • , Sean J. Humphrey
    • , Guang Yang
    • , Deanne Francis
    • , Sabina Yasmin
    • , Sung-Young Shin
    • , Dougall M. Norris
    • , Alison L. Kearney
    • , Miro A. Astore
    • , Jonathan Scavuzzo
    • , Kelsey H. Fisher-Wellman
    • , Qiao-Ping Wang
    • , Benjamin L. Parker
    • , G. Gregory Neely
    • , Fatemeh Vafaee
    • , Joyce Chiu
    • , Reichelle Yeo
    • , Philip J. Hogg
    • , Daniel J. Fazakerley
    • , Lan K. Nguyen
    • , Serdar Kuyucak
    •  & David E. James
  • Article
    | Open Access

    Higher organisms regulate cellular iron concentrations through Iron Regulatory Proteins (IRPs), which regulate specific messenger RNAs. Here Huynh et al. show that IRP1 requires a Glycogen Branching Enzyme for proper function, and that IRP1 has additional regulatory roles in cell nuclei.

    • Nhan Huynh
    • , Qiuxiang Ou
    • , Pendleton Cox
    • , Roland Lill
    •  & Kirst King-Jones
  • Article
    | Open Access

    Liquid-liquid phase separation (LLPS) of intrinsically disordered proteins plays an important part in the formation of extracellular biological materials. Here, the authors show that repeats of the peptide motif GHGLY are necessary for the LLPS of pH-responsive histidine-rich squid beak proteins.

    • Bartosz Gabryelczyk
    • , Hao Cai
    • , Xiangyan Shi
    • , Yue Sun
    • , Piet J. M. Swinkels
    • , Stefan Salentinig
    • , Konstantin Pervushin
    •  & Ali Miserez
  • Article
    | Open Access

    Viral genomic DNA is often modified to evade the host bacterial restriction system. Here the authors identified 2′-deoxy-7-deazaguanine modifications on phage DNA by comparative genomics and experimental validation, showing their role in genome protection.

    • Geoffrey Hutinet
    • , Witold Kot
    • , Liang Cui
    • , Roman Hillebrand
    • , Seetharamsingh Balamkundu
    • , Shanmugavel Gnanakalai
    • , Ramesh Neelakandan
    • , Alexander B. Carstens
    • , Chuan Fa Lui
    • , Denise Tremblay
    • , Deborah Jacobs-Sera
    • , Mandana Sassanfar
    • , Yan-Jiun Lee
    • , Peter Weigele
    • , Sylvain Moineau
    • , Graham F. Hatfull
    • , Peter C. Dedon
    • , Lars H. Hansen
    •  & Valérie de Crécy-Lagard
  • Article
    | Open Access

    How genome stability is maintained at regions of active transcription is currently not entirely clear. Here, the authors reveal an association between base excision repair factors and transcription elongation to modulate DNA repair.

    • Nicola P. Montaldo
    • , Diana L. Bordin
    • , Alessandro Brambilla
    • , Marcel Rösinger
    • , Sarah L. Fordyce Martin
    • , Karine Øian Bjørås
    • , Stefano Bradamante
    • , Per Arne Aas
    • , Antonia Furrer
    • , Lene C. Olsen
    • , Nicolas Kunath
    • , Marit Otterlei
    • , Pål Sætrom
    • , Magnar Bjørås
    • , Leona D. Samson
    •  & Barbara van Loon
  • Article
    | Open Access

    DNA ligases catalyze the joining of DNA strands to complete DNA replication, recombination and repair transactions. Here the authors present X-ray structures and kinetic analyses of LIG1 complexes with undamaged and oxidatively damaged DNA that unveil determinants of LIG1 substrate recognition and enzymatic fidelity.

    • Percy P. Tumbale
    • , Thomas J. Jurkiw
    • , Matthew J. Schellenberg
    • , Amanda A. Riccio
    • , Patrick J O’Brien
    •  & R. Scott Williams
  • Article
    | Open Access

    It remains challenging to estimate carbon accumulation rates in tidal wetlands on a scale as large as the conterminous US. Here, the authors find that mean C accumulation rates vary greatly among watershed regions but not among vegetation types, and that tidal wetlands’ C sequestration capability will remain or increase by 2100, suggesting a resilience to sea level rise.

    • Faming Wang
    • , Xiaoliang Lu
    • , Christian J. Sanders
    •  & Jianwu Tang
  • Article
    | Open Access

    Bacterial type II secretion systems (T2SSs) translocate virulence factors, toxins and enzymes across the cell outer membrane. Here, Chernyatina and Low use negative stain and cryo-electron microscopy to reveal the core architecture of an assembled T2SS from the pathogen Klebsiella pneumoniae.

    • Anastasia A. Chernyatina
    •  & Harry H. Low
  • Article
    | Open Access

    Polar-faceted nanocrystals often have complex atomic surface configurations that are very difficult to characterize. Here, the authors are able to determine detailed structural information about the polar facets of ceria nanocubes by using 17O and 1H solid-state NMR spectroscopy, in combination with density functional theory calculations.

    • Junchao Chen
    • , Xin-Ping Wu
    • , Michael A. Hope
    • , Kun Qian
    • , David M. Halat
    • , Tao Liu
    • , Yuhong Li
    • , Li Shen
    • , Xiaokang Ke
    • , Yujie Wen
    • , Jia-Huan Du
    • , Pieter C. M. M. Magusin
    • , Subhradip Paul
    • , Weiping Ding
    • , Xue-Qing Gong
    • , Clare P. Grey
    •  & Luming Peng
  • Article
    | Open Access

    The human ATP-binding cassette transporter ABCG2 plays critical roles in anticancer resistance but the molecular mechanism of ABCG2-mediated substrate transport remains enigmatic. Here authors use extensive mutagenesis and molecular dynamics simulations to reveal a mechanistic basis for the function of the di-leucine valve and the roof organization in the transport cycle.

    • Narakorn Khunweeraphong
    • , Daniel Szöllősi
    • , Thomas Stockner
    •  & Karl Kuchler
  • Article
    | Open Access

    Established bacterial glycoengineering platforms limit access to protein and glycan substrates. Here the authors design a cytoplasmic protein glycosylation system, Glycoli, to generate a variety of multivalent glycostructures.

    • Hanne L. P. Tytgat
    • , Chia-wei Lin
    • , Mikail D. Levasseur
    • , Markus B. Tomek
    • , Christoph Rutschmann
    • , Jacqueline Mock
    • , Nora Liebscher
    • , Naohiro Terasaka
    • , Yusuke Azuma
    • , Michael Wetter
    • , Martin F. Bachmann
    • , Donald Hilvert
    • , Markus Aebi
    •  & Timothy G. Keys
  • Article
    | Open Access

    Constructing biosynthetic pathways to study and engineer glycoprotein structures is difficult. Here, the authors use GlycoPRIME, a cell-free workflow for mixing-and-matching glycosylation enzymes, to evaluate 37 putative glycosylation pathways and discover routes to 18 new glycoprotein structures

    • Weston Kightlinger
    • , Katherine E. Duncker
    • , Ashvita Ramesh
    • , Ariel H. Thames
    • , Aravind Natarajan
    • , Jessica C. Stark
    • , Allen Yang
    • , Liang Lin
    • , Milan Mrksich
    • , Matthew P. DeLisa
    •  & Michael C. Jewett
  • Article
    | Open Access

    Cytotoxic T lymphocytes (CTLs) eliminate virus-infected and cancerous cells by secreting the pore-forming protein (perforin) and pro-apoptotic serine proteases (granzymes). Here authors show that two mechanisms protect the membranes of CTLs from disruption by perforin and granzymes.

    • Jesse A. Rudd-Schmidt
    • , Adrian W. Hodel
    • , Tahereh Noori
    • , Jamie A. Lopez
    • , Hyun-Jung Cho
    • , Sandra Verschoor
    • , Annette Ciccone
    • , Joseph A. Trapani
    • , Bart W. Hoogenboom
    •  & Ilia Voskoboinik
  • Article
    | Open Access

    The coordination of interactions between multiple regulatory elements and genes within a chromatin domain remains poorly understood. Here, the authors use a method to detect multi-way chromatin interactions in a mouse model in which the α-globin domain is extended to include several additional genes, finding that the promoters do not form mutually exclusive interactions with the enhancers, but all interact simultaneously in a single complex.

    • A. Marieke Oudelaar
    • , Caroline L. Harrold
    • , Lars L. P. Hanssen
    • , Jelena M. Telenius
    • , Douglas R. Higgs
    •  & Jim R. Hughes
  • Article
    | Open Access

    Myeloid-derived growth factor (MYDGF) is an angiogenic growth factor with therapeutic potential for ischemic tissue repair and the receptor is still unknown. Here the authors present the crystal structure of human MYDGF and identify its functional epitope through mutagenesis studies.

    • Rebecca Ebenhoch
    • , Abbas Akhdar
    • , Marc R. Reboll
    • , Mortimer Korf-Klingebiel
    • , Priyanka Gupta
    • , Julie Armstrong
    • , Yining Huang
    • , Lee Frego
    • , Irina Rybina
    • , John Miglietta
    • , Anton Pekcec
    • , Kai C. Wollert
    •  & Herbert Nar
  • Article
    | Open Access

    Here, the authors identify a broadly neutralizing antibody from an HIV-infected person that recognizes the membrane-proximal external region (MPER) of HIV envelope glycoprotein (Env) and has a short CDRH3 and low polyreactivity. Structural analysis shows how the antibody binds the MPER and Env on the viral membrane.

    • Lei Zhang
    • , Adriana Irimia
    • , Lingling He
    • , Elise Landais
    • , Kimmo Rantalainen
    • , Daniel P. Leaman
    • , Thomas Vollbrecht
    • , Armando Stano
    • , Daniel I. Sands
    • , Arthur S. Kim
    • , George Miiro
    • , Jennifer Serwanga
    • , Anton Pozniak
    • , Dale McPhee
    • , Oliver Manigart
    • , Lawrence Mwananyanda
    • , Etienne Karita
    • , André Inwoley
    • , Walter Jaoko
    • , Jack DeHovitz
    • , Linda-Gail Bekker
    • , Punnee Pitisuttithum
    • , Robert Paris
    • , Susan Allen
    • , Pascal Poignard
    • , Dennis R. Burton
    • , Ben Murrell
    • , Andrew B. Ward
    • , Jiang Zhu
    • , Ian A. Wilson
    •  & Michael B. Zwick