Biochemistry

Definition

Biochemistry is the study of the structure and function of biological molecules such as proteins, nucleic acids, carbohydrates and lipids. Biochemistry is also used to describe techniques suited to understanding the interactions and functions of biological molecules, including traditional techniques such as Western blotting, co-immunoprecipitation, and chromatography methods.

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Latest Research and Reviews

  • Protocols |

    This protocol enables the study of protein complexes by trapping them in virus-like particles and thereby preserving cellular integrity. The Virotrap protocol is complemented by SFINX, an online data analysis tool for interactomics studies.

    • Kevin Titeca
    • , Emmy Van Quickelberghe
    • , Noortje Samyn
    • , Delphine De Sutter
    • , Annick Verhee
    • , Kris Gevaert
    • , Jan Tavernier
    •  & Sven Eyckerman
    Nature Protocols 12, 881–898
  • Protocols |

    Li et al. provide a protocol for long-read ChIA-PET, a technique for mapping chromatin interactions. The longer paired-end tags, which are generated by tagmentation, provide sufficient coverage to determine haplotype-specific chromatin interactions at single-nucleotide resolution.

    • Xingwang Li
    • , Oscar Junhong Luo
    • , Ping Wang
    • , Meizhen Zheng
    • , Danjuan Wang
    • , Emaly Piecuch
    • , Jacqueline Jufen Zhu
    • , Simon Zhongyuan Tian
    • , Zhonghui Tang
    • , Guoliang Li
    •  & Yijun Ruan
    Nature Protocols 12, 899–915
  • Reviews |

    Lipid rafts are relatively ordered membrane domains that are enriched in cholesterol and saturated lipids, and selectively recruit other lipids and proteins. They are dynamic and heterogeneous in composition and are thus challenging to visualize in vivo. New technologies are providing novel insights into the formation, organization and functions of these membrane domains.

    • Erdinc Sezgin
    • , Ilya Levental
    • , Satyajit Mayor
    •  & Christian Eggeling
  • Research |

    Using a single-nucleus Hi-C protocol, the authors find that spatial organization of chromatin during oocyte-to-zygote transition differs between paternal and maternal nuclei within a single-cell zygote.

    • Ilya M. Flyamer
    • , Johanna Gassler
    • , Maxim Imakaev
    • , Hugo B. Brandão
    • , Sergey V. Ulianov
    • , Nezar Abdennur
    • , Sergey V. Razin
    • , Leonid A. Mirny
    •  & Kikuë Tachibana-Konwalski
  • Research | | open

    Allostery and drug-drug synergism can yield potential novel therapies with existing molecules. Here, the authors provide evidence that two unrelated drugs have increased cytotoxicity in cancer cells, which coincides with increased formation of chromatin adducts.

    • Zenita Adhireksan
    • , Giulia Palermo
    • , Tina Riedel
    • , Zhujun Ma
    • , Reyhan Muhammad
    • , Ursula Rothlisberger
    • , Paul J. Dyson
    •  & Curt A. Davey

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