Antibody isolation and purification

Antibody isolation and purification is the concentration of an antibody or antibodies that recognize a specific target molecule and the removal of non-specific antibodies and contaminants. Pure isolated antibodies tend to have greater activity and are less likely to cross-react with non-target molecules than impure mixtures.

Latest Research and Reviews

  • Research |

    A collection of 1,406 high-quality, immunoprecipitation- and immunoblotting-grade monoclonal antibodies to 737 human transcription factors is made available as a community resource, along with all validation data.

    • Anand Venkataraman
    • , Kun Yang
    • , Jose Irizarry
    • , Mark Mackiewicz
    • , Paolo Mita
    • , Zheng Kuang
    • , Lin Xue
    • , Devlina Ghosh
    • , Shuang Liu
    • , Pedro Ramos
    • , Shaohui Hu
    • , Diane Bayron Kain
    • , Sarah Keegan
    • , Richard Saul
    • , Simona Colantonio
    • , Hongyan Zhang
    • , Florencia Pauli Behn
    • , Guang Song
    • , Edisa Albino
    • , Lillyann Asencio
    • , Leonardo Ramos
    • , Luvir Lugo
    • , Gloriner Morell
    • , Javier Rivera
    • , Kimberly Ruiz
    • , Ruth Almodovar
    • , Luis Nazario
    • , Keven Murphy
    • , Ivan Vargas
    • , Zully Ann Rivera-Pacheco
    • , Christian Rosa
    • , Moises Vargas
    • , Jessica McDade
    • , Brian S Clark
    • , Sooyeon Yoo
    • , Seva G Khambadkone
    • , Jimmy de Melo
    • , Milanka Stevanovic
    • , Lizhi Jiang
    • , Yana Li
    • , Wendy Y Yap
    • , Brittany Jones
    • , Atul Tandon
    • , Elliot Campbell
    • , Gaetano T Montelione
    • , Stephen Anderson
    • , Richard M Myers
    • , Jef D Boeke
    • , David Fenyö
    • , Gordon Whiteley
    • , Joel S Bader
    • , Ignacio Pino
    • , Daniel J Eichinger
    • , Heng Zhu
    •  & Seth Blackshaw
    Nature Methods 15, 330-338
  • Research | | open

    Bispecific antibodies allow for novel therapeutic approaches but industrial-scale production and immunogenicity represent significant challenges. Here Fischer et al. describe a unique human bispecific antibody format that exploits differing light chains to overcome these obstacles.

    • Nicolas Fischer
    • , Greg Elson
    • , Giovanni Magistrelli
    • , Elie Dheilly
    • , Nicolas Fouque
    • , Amélie Laurendon
    • , Franck Gueneau
    • , Ulla Ravn
    • , Jean-François Depoisier
    • , Valery Moine
    • , Sylvain Raimondi
    • , Pauline Malinge
    • , Laura Di Grazia
    • , François Rousseau
    • , Yves Poitevin
    • , Sébastien Calloud
    • , Pierre-Alexis Cayatte
    • , Mathias Alcoz
    • , Guillemette Pontini
    • , Séverine Fagète
    • , Lucile Broyer
    • , Marie Corbier
    • , Delphine Schrag
    • , Gérard Didelot
    • , Nicolas Bosson
    • , Nessie Costes
    • , Laura Cons
    • , Vanessa Buatois
    • , Zoe Johnson
    • , Walter Ferlin
    • , Krzysztof Masternak
    •  & Marie Kosco-Vilbois

News and Comment