A Gate Hinge Controls the Epithelial Calcium Channel TRPV5

van der Wijst, Jenny; Leunissen, Elizabeth H.; Blanchard, Maxime G.; Venselaar, Hanka; Verkaart, Sjoerd; Paulsen, Candice E.; Bindels, René J.; Hoenderop, Joost G.

doi:10.1038/srep45489

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Published: 04 April 2017

Scientific Reports volume 7, Article number: 45489 (2017) Cite this article

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Abstract

TRPV5 is unique within the large TRP channel family for displaying a high Ca²⁺ selectivity together with Ca²⁺-dependent inactivation. Our study aims to uncover novel insights into channel gating through in-depth structure-function analysis. We identify an exceptional tryptophan (W583) at the terminus of the intracellular pore that is unique for TRPV5 (and TRPV6). A combination of site-directed mutagenesis, biochemical and electrophysiological analysis, together with homology modeling, demonstrates that W583 is part of the gate for Ca²⁺ permeation. The W583 mutants show increased cell death due to profoundly enhanced Ca²⁺ influx, resulting from altered channel function. A glycine residue above W583 might act as flexible linker to rearrange the tryptophan gate. Furthermore, we hypothesize functional crosstalk between the pore region and carboxy terminus, involved in Ca²⁺-calmodulin-mediated inactivation. This study proposes a unique channel gating mechanism and delivers detailed molecular insight into the Ca²⁺ permeation pathway that can be extrapolated to other Ca²⁺-selective channels.

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Introduction

The transient receptor potential (TRP) family comprises ion channels with similar structures but with diverse functional properties¹. TRP channels are composed of four subunits, each containing six transmembrane (TM) segments and intracellular amino (N)- and carboxyl (C)-termini^2,3, thereby resembling voltage-gated potassium (K_v) and bacterial sodium (Na_v) channels. Over the last two decades, structural studies on several K_v channels have given new insights into channel composition and function⁴. In contrast, research on TRP channel structure-function relation is still in its infancy due to the minimal structural information available.

Within the TRP family, TRPV5 (vanilloid type 5) and its close homologue TRPV6 form a distinct class as the most calcium (Ca²⁺)-selective channel members⁵. TRPV5 constitutes the apical gate for transepithelial Ca²⁺ reabsorption in the kidney and is primarily expressed in the late distal convoluted tubule and connecting tubule of the nephron^6,7. It is characterized as a constitutively active channel, with a substantial Ca²⁺ permeability at physiological membrane potentials^8,9. TRPV5 exhibits a selectivity filter sequence consisting of a ring of four aspartic acid residues (D542) that forms the main extracellular Ca²⁺-binding pocket^10,11. This residue is crucial to many channel characteristics including a high Ca²⁺ permeability, block by magnesium (Mg²⁺), and Ca²⁺-dependent current decay¹¹.

The rate of TRPV5 channel inactivation directly correlates with the Ca²⁺ flow through the channel. A high Ca²⁺ level in close vicinity to the intracellular channel mouth functions as a negative feedback mechanism and inhibits TRPV5 channel activity^12,13. Calmodulin (CaM), a ubiquitous Ca²⁺-sensor protein, mediates part of the Ca²⁺-dependent inactivation by binding to the C-terminus of TRPV5^14,15. High intracellular Ca²⁺ levels enhance the CaM binding¹⁵. Removal of the C-terminal fragment of TRPV5 (S698X) abolishes the sensitivity for CaM, resulting in enhanced Ca²⁺-influx due to decreased Ca²⁺-dependent inactivation^15,16,17. Despite these insights, the current knowledge about the gating mechanism of TRPV5 and other Ca²⁺-selective channels at the single molecule level is limited.

The recent elucidation of three TRPV channel structures (TRPV1, TRPV2, and TRPV6) has provided the first insight into channel architecture and possible gating mechanisms^18,19,20,21. These structures unveil a tetrameric channel topology that has a symmetrical arrangement of four subunits around the central ion conduction pathway. This pathway contains two constrictions (or gates): an upper residue that forms the selectivity filter in the outer pore region and a lower gate at the inner end of TM6^18,19,20. These two constrictions were also observed in the recent TRPA1 structure²². While the structure of the outer pore domain encompassing the selectivity filter has been well established for these TRPV channels, and likely explain the divergence in TRP channel activation¹⁹, there is still debate about the lower gate. This is formed by helical bundle crossing of TM6. In TRPV1, this constriction is formed by an isoleucine residue (TRPV1 I679) that is conserved among TRPV members (Fig. 1b)¹⁸. Mutation of this residue (I679A) resulted in decreased capsaicin currents²³. A structurally equivalent isoleucine residue also contributes to the lower constriction in TRPA1 suggesting conservation among other TRP channels²². A recent study on the TRPV2 structure postulates a methionine as the lower constriction point (TRPV2 M643), which is also shown by a later study demonstrating the TRPV6 crystal structure (TRPV6 M577)^19,20.

**Figure 1: Effect of W583 mutations on TRPV5 function.**

In the present study, we aligned this TM6 region in TRPV5 with all TRPV channels and screened for the functional role of the putative restriction points by mutation analysis. In contrast to the postulated lower gate residues, we identify a conserved (in TRPV5/6) tryptophan residue (W583) at the intracellular mouth of the pore that, when mutated, severely affects TRPV5 channel function. In addition, our data demonstrates a conserved glycine hinge in close proximity to W583 that provides flexibility to the lower tryptophan (W583) gate. Furthermore, we reveal crosstalk between the glycine hinge and the C-terminus that is involved in CaM-mediated channel inactivation. The present study provides novel mechanistic understanding of unique structural requirements for TRPV5 channel gating.

Results

Comparison of the TRPV channel pores

The flow of Ca²⁺ through TRPV5 is expected to involve two steps. First, Ca²⁺ ions are attracted by the negative charge of D542 at the outer surface of the pore, which serves as the selectivity filter¹¹. Second, the lower gate at the intracellular end of the pore region of TRPV5 can determine the influx of Ca²⁺. To determine whether homologous residues of the postulated lower gate (I575 and M578) play a role in TRPV5 Ca²⁺ permeation, we evaluated channel function of I575A and M578A mutants by ⁴⁵Ca²⁺ uptake assay. The I575A mutant did not show altered channel expression or function (Fig. 1a), which was expected if I575 is considered the channel gate. The M578A mutant did not demonstrate complete loss of function, pointing towards involvement of additional residues in the lower gate. As TRPV5 exhibits a unique high Ca²⁺-selectivity, its channel gating might be differently regulated compared to other TRPV channels. Interestingly, TRPV5 contains a histidine residue (H582) and tryptophan residue (W583) that are highly conserved among different species and in TRPV6, but not in other members of the TRPV family (Fig. 1b). The ⁴⁵Ca²⁺ uptake assay demonstrated a significantly reduced uptake of H582 and W583 compared to wildtype TRPV5. Given the hydrophobic nature of the latter amino acid, we hypothesized that the position of this bulky amino acid might be energetically unfavorable for ion permeation depending on side chain conformation.

Mutation of W583 increases the Ca²⁺ permeability of TRPV5

Site-directed mutagenesis was applied to substitute W583 for amino acids with different characteristics (aromatic: W583Y and W583F; non aromatic: W583Q, W583L and W583A). The activity of the mutant channels was first assessed by a ⁴⁵Ca²⁺ uptake assay. Ruthenium red (RR) was used to measure the TRPV5-mediated component of the ⁴⁵Ca²⁺ influx²⁴. A significantly diminished ⁴⁵Ca²⁺ uptake was observed in all mutants compared to wild type TRPV5 (Fig. 1c). However, TRPV5 protein expression was also strongly reduced in cells expressing the different mutants (Fig. 1d). This results from an observed decrease in cell survival, which is confirmed by a trypan blue assay demonstrating significantly increased cell mortality for all mutants compared to wild type TRPV5 (Fig. 1e). To establish a link between enhanced Ca²⁺ uptake and reduced cell survival, the basal intracellular Ca²⁺ levels were measured using fura-2-AM, a cell-permeant ratiometric fluorescent Ca²⁺ indicator. Using this strategy, a significant increase in baseline Ca²⁺ levels was detected in cells expressing mutant TRPV5 (Fig. 1f).

To demonstrate that the observed cell death is a result of increased TRPV5-mediated Ca²⁺ influx, we mutated the selectivity filter (D542A) that is known to ablate Ca²⁺ permeation of the channel¹¹. The ⁴⁵Ca²⁺ uptake of D542A/W583L was not different from D542A, confirming the block of Ca²⁺ permeation (Fig. 1g). In contrast to previous experiments, the ⁴⁵Ca²⁺ uptake was corrected for protein expression in order to demonstrate differences in TRPV5 activity since the expression of the single mutant W583L was nearly absent on the immunoblot, while the double mutant D542A/W583L is expressed at similar levels as wild type TRPV5 and D542A (Fig. 1g). To demonstrate channel expression at the plasma cell membrane, a cell surface biotinylation was performed using cells expressing wild type TRPV5, or either single or double mutants. Similar to Fig. 1d, the overall protein expression (in both biotin and input fraction) of W583L was severely diminished. This was rescued by the D542A mutation since the double D542A/W583L mutant showed equal plasma membrane abundance to wild type TRPV5 and D542A (Fig. 1h).

Mutation of W583 affects the intrinsic channel properties of TRPV5

Next, a non-aromatic and an aromatic TRPV5 mutant (W583L and W583F) were subjected to whole-cell patch clamp recording to examine their channel characteristics using a voltage step protocol (from −100 to 40 mV). Figure 2a–c show representative traces of wild type TRPV5, W583L and W583F at the voltage step to −100 mV. The current-voltage relationship is established from the voltage step protocol and is similar between mutant and wild type TRPV5 (Fig. 2d). Importantly, the mutant channels exhibit the characteristic Na⁺ inward-rectifying current in nominally divalent-free (nDVF) bath solution, which is enhanced upon addition of EGTA-containing solution (DVF, 100 μM EGTA) (Fig. 2a–c). The overall deleterious effect of mutant TRPV5 expression in HEK293 cells did not allow for accurate quantification and comparison of current densities between mutant and wild type TRPV5. Of note, the absolute currents of both mutants were 3–4 fold smaller than wild type TRPV5.

**Figure 2: Channel activity is altered in the TRPV5 W583 mutants.**

In order to assess putative changes in the intrinsic properties of the mutant TRPV5 channels, cells expressing wild type or mutant TRPV5 were subjected to cell-attached patch clamp. Briefly, TRPV5 activity was measured by applying a series of 10 s voltage steps to −80 mV from a holding voltage of 0 mV, using Na⁺ as the charge carrier. By applying this protocol, frequent patches containing one to three channels were obtained and analyzed for amplitude of openings and open probability. Representative channel openings and amplitude histograms of wild type, W583L and W583F are depicted in Fig. 2f–h. While the aromatic W583F showed a decreased open probability compared to wild type TRPV5, the non-aromatic W583L mutant presented a tendency of increased single channel activity (Fig. 2e). Analysis of other mutant recordings also revealed a trend towards increased open probability for W583Y, W583A, W583Q (significant) compared to wild type TRPV5 (Fig. S1A,B). The amplitude of opening at −80 mV was not significantly different between wild type TRPV5 and the mutants (Fig. S1C).

In order to provide more insight into the kinetics of the single channel behavior, we compared wild type, W583F and W583L in our model-based fitting of the duration histograms and subsequent dwell-time analysis of the open and closed states. We hypothesized a three-state model (O ↔ C2 ↔ C1): an open state (O), closed state (C1), and pre-open closed state (C2), as detailed analysis demonstrated that one open and two closed exponential components are required to describe the open and closed dwell-time distributions. The average time constants of the open and closed states were significantly reduced in W583L compared to wild type TRPV5, which indicates that the channel switches faster between states (Fig. 2i). This was confirmed by a significantly increased closing rate constant for W583L (Fig. S1D). The W583F mutant was not significantly different from wild type TRPV5 apart from an increased time constant in the closed state (C1) that could explain the decreased open probability (Fig. 2e,i).

Detailed insight into the TRPV5 pore

To investigate a structural basis for TRPV5 channel gating, we built a homology model of TRPV5 using the recently determined structure of rat TRPV1 (16) (Fig. 3a, Fig. S2A). According to the Sander-Schneider plot, the 29% identity between rabbit TRPV5 and rat TRPV1 is sufficient to perform homology modeling²⁵. The model represents the tetrameric structure of TRPV5 with the putative transmembrane core surrounding a central cavity for ion permeation. First, the molecular surface representation shows the localization and orientation of the D542 residue as a negatively charged ring at the entry of the channel (Fig. S2B)^11,26. Interestingly, the inner pore region contains the tryptophan residue (W583) residue at the intracellular end of TM6, with its side chain pointing towards the ion permeation pathway (Fig. 3b and d, Fig. S2B,C). Given the hydrophobic nature of the water facing tryptophan side chain, this conformation appears energetically unfavorable. However, the side chain of a tryptophan can convert to different rotameric positions, which often depends on the backbone conformation²⁷. The YASARA & WHAT IF Twinset was used to extract the preferred rotamers and the associated probabilities, depicted as different colors in Fig. 3c (left panel). The three main rotameric positions, with the side chains either facing up, down or towards each other, demonstrate a considerable degree of variation in ion permeation space. When the W583 side chains are facing each other, the estimated distance between them is ~3.1 Å, and therefore, the estimated passage size is around 0.8 Å, while the other two rotamers form a wider pore (Fig. 3c).

G579 forms an inner pore helix hinge in TRPV5

Based on the altered function of the W583 mutants, we hypothesize that the tryptophan serves as channel gate similar to what is observed in some voltage-gated K⁺ channels²⁸. Interestingly, prokaryotic K⁺ channel gating is often mediated by glycine helix kinking in combination with an aromatic gate²⁹. In TRPV5, a glycine (G579), one α-helical turn above W583, could serve as a flexible hinge point for movement of the lower TM6 helix allowing the W583 residues to adopt different conformations (Fig. 4a). In order to test this hypothesis, G579 was mutated into the more rigid alanine residue. The Ca²⁺ permeability of G579A is significantly reduced (100 ± 2% and 41 ± 3% for wild type and G579A mutant, respectively) as demonstrated by a ⁴⁵Ca²⁺ uptake assay (Fig. 4b). Total protein expression was not altered compared to wild type (Fig. 4b). In addition, cell surface biotinylation assays showed that TRPV5 G579A is present at the plasma membrane, albeit at slightly reduced levels (100 ± 4% and 72 ± 16% for wild type and G57A mutant, respectively) (Fig. S3A). Whole-cell patch clamp recordings demonstrated a significantly diminished current density for G579A, with no difference in the shape of the current/voltage relationship or the EGTA-sensitive current (Fig. 4c,d and Fig. S3B). Cell-attached single channel analysis of the G579A mutant revealed a decreased open probability (3.0 ± 0.6% and 21 ± 4% for G579A and wild type, respectively) (Fig. 4e). Of note, the amplitude of opening was not significantly altered (Fig. 4e, 5.5 ± 0.3 pA for wild type vs 4.8 ± 0.9 pA for G579A). The G579A mutation renders the channel less functional and we hypothesized that it could reduce the severe phenotype of the W583 mutants.

**Figure 4: Analysis of the G579A mutation on TRPV5 activity.**

To investigate whether G579A is able to rescue the cell death caused by mutation of W583, three TRPV5 double mutants (G579A-W583Y, G579A-W583F, G579A-W583A) were generated. Interestingly, TRPV5 protein expression of the double mutants containing the aromatic substitutions (G579A-W583Y and G579A-W583F) was noticeably enhanced compared to the single W583 mutants, whereas the phenotype of W583A could not be rescued by addition of the G579A mutation (Fig. 4f, upper panel). Though it seems that Ca²⁺ permeability was not restored, as the ⁴⁵Ca²⁺ uptake was still significantly reduced compared to wild type (WT) TRPV5, similarly as the single W583 mutants (Fig. 4f, lower panel).

Linking the carboxy-terminus and the pore

Removal of the TRPV5 C-terminal domain (S698X) or ablating its CaM binding site (W702A) is also known to result in enhanced Ca²⁺ influx as a result of diminished CaM-mediated Ca²⁺-dependent channel inactivation^15,16. Hence, we aimed to investigate whether there is a structural crosstalk between the C-terminus and the pore. The G579A mutation was introduced in the TRPV5 S698X and W702A mutants. Protein expression of both the G579A-S698X and G579A-W702A mutants was increased compared to their single mutant counterparts (Fig. 5b). Moreover, it normalized TRPV5 function since ⁴⁵Ca²⁺ uptake levels of both double mutants were comparable to wild type, while the single mutants demonstrated significantly lower ⁴⁵Ca²⁺ uptake due to the reduced cell survival (Fig. 5a). Protein expression and function could not be restored in the G579A-W583A mutant. In line with previous findings, the basal intracellular Ca²⁺ levels were significantly increased in the TRPV5 S698X and W702A mutants^15,17 and we now demonstrated that addition of G579A to these mutants also led to normal Ca²⁺ levels as measured using fura-2-AM (Fig. 5c). Interestingly, this seems independent from CaM binding as functionalized CaM agarose beads were able to bind wild type TRPV5 and the G579A mutant, but not S698X and W702A single or G579A-S698X and G579A-W702A double mutants (Fig. 5d). Of note, binding to CaM beads occurred only in the presence of Ca²⁺, as EGTA-containing buffer will prevent CaM interaction (Fig. 5d). The D542A and D542A-W583L single and double mutants were used as controls to demonstrate that mutation of W583 does not affect the CaM interaction.

**Figure 5: Rescue the TRPV5 CaM binding mutants by the G579A mutation.**

Discussion

This study presents a novel residue (W583) in TRPV5, which affects channel gating and is postulated as an aromatic gate that functions as the final determinant in Ca²⁺ permeation through the channel. Structural movement of this lower gate could be mediated by a nearby glycine hinge (G579), which in turn might play a role in CaM-dependent channel inactivation. Based on our TRPV5 homology model, the rotameric conformations of W583 were analyzed and three major side chain positions were detected: pointing down, pointing up or facing each other. Often, aromatic residues are localized at the interfacial regions of a TM α-helix and the solvent. These residues have a role in anchoring proteins into the cell membrane through interactions with the lipid head groups³⁰. In contrast, the current TRPV5 model shows that W583 is located at the intracellular face and it is pointing away from the lipids. This observation, together with the present findings, suggests that W583 does not participate in the interaction of the α-helix with lipids, but may play a role in channel gating. As a result of their size and shape, a small rotation in the aromatic residue can lead to a large change in width of the gate²⁸. When the W583 side chains are facing each other, the selective passage of Ca²⁺ is hampered since the estimated pore diameter in this conformation is ~0.8 Å and dehydrated Ca²⁺ has a diameter of 0.99 Å³¹. Upon conformational changes, the side chains can be placed into the other rotameric positions, widening the gate radius to ~11.2 Å, and thereby allowing Ca²⁺ passage. When we characterize the ion conduction pathway of our TRPV5 model, we find that the conserved I575 does not form a constriction point contributing to the lower gate. In contrast, the homologous I679 in TRPV1 forms a constriction (5.3 Å) that blocks ion permeation in the closed state^18,32. Also M578, which is the homologue of the postulated lower gate (M577) in TRPV6, was not identified as major contribution to pore constriction in our model. To approve our data in the context of the recently resolved TRPV6 structure, we have also generated a TRPV5 homology model based on TRPV6 structure (Fig. S2D,E). In both models, we see that W583 lies at the intracellular mouth of the ion conduction pore, and propose that by rotation of its side chain into the ion conduction pathway could form a tight constriction. Homology modeling is a valuable method for structure prediction in the absence of a resolved protein structure, but it is important to remain cautious for potential inaccuracies. Our conclusions about the roles of amino acids are qualitative and not quantitative. Energy refinement methods during model building might force the channel into artificial positions. Hence, the homology model serves as supportive structural material for our experimental data that highlight the importance of W583 in TRPV5 channel function.

A gating function for aromatic residues has been studied in other ion channels. The proton-selective influenza virus M₂ channel is regulated by a so-called tryptophan gate³³. This tryptophan gate is stabilized by aspartate residues through a network of hydrogen bonds, which support the closed state conformation of the channel²⁹. Upon lowering the outside pH, four histidine residues in this network are protonated, leading to a conformation change of the gate. It has been described for the prokaryotic K⁺ channel KcsA that step-wise conformational changes occur at F103, as a function of channel opening. The position of the side chain of F103 changes upon inner helix movement and is associated with opening of the upper activation gate³⁴. Moreover, mutations at this phenylalanine gate have a size-dependent effect on the gating kinetics. Small side-chain substitutions result in impaired inactivation kinetics, while substitution with larger side chain residues has minor effects on channel characteristics³⁴. In line with this finding, our study demonstrated that substitution of W583 with a non-aromatic residue (W583Q, W583L, and W583A) results in massive ⁴⁵Ca²⁺ influx and cell death, while change to an aromatic residue (W583F and W583Y) have a milder phenotype. Interestingly, the channel’s open probability of W583F is decreased compared to wild type TRPV5. It should be noted that cell-attached currents are recorded in Ca²⁺-free solution containing Na⁺ as charge carrier. Our hypothesis is that the side chain of the phenylalanine can still close the channel gate to some extent as apparent from the dwell time analysis, thereby allowing passage of the smaller Ca²⁺ ions but rendering the channel less permeable for Na⁺.

Several mechanisms have been proposed for the gating of voltage-gated K⁺ channels²⁸: (i) rotation of the inner pore helix around the pore axis, (ii) kinking of the inner pore helix, and (iii) helix kinking in combination with an aromatic gate. Most eukaryotic K⁺ channels are regulated by kinking of the inner pore helix at a proline-valine-proline (PVP) sequence³⁵, a motif that is absent in TRPV5. Prokaryotic K⁺ channel gating is often mediated through an aromatic gate in combination with helix kinking by a flexible hinge point at the bottom of the α-helix that is composed of a glycine^28,35. The prokaryotic K⁺ inwardly rectifying KirBac 1.1 channel functions through four phenylalanine residues at the cytoplasmic end of the pore³⁶, which are displaced by bending a so-called gating hinge (a glycine) in the inner pore helices, thereby allowing channel opening^28,37. A similar gating mechanism has been proposed for the closely related KirBac 3.1 channel, using a tyrosine residue³⁸. The current TRPV5 homology model reveals a highly conserved glycine (G579), located one α-helical turn above the aromatic gate that may produce such a helix kink. Mutation of G579 into an alanine completely blocked the ⁴⁵Ca²⁺ uptake by reducing the open probability, leading to the hypothesis that G579 is essential for proper channel gating. Substitution of this residue is suggested to hold the channel into a closed state. On contrary, mutation to proline could potentially bend the helix and keep the channel open, however, a rather flickering single channel behavior was observed (data not shown).

Importantly, future studies will aim at determining the 3D structure of TRPV5 to resolve this potentially unique pore and provide further insight into channel gating mechanisms. A key question is which stimuli trigger the conformational change that precedes channel gating. For example, the TRPV5 TM6 α-helix changes its conformation upon extracellular acidification³⁹. In addition, the pore helix is rotated along its axis as a result of intra- or extracellular acidification⁴⁰. A pH-sensitive histidine (582) is located directly above the tryptophan gate. Protonation of the histidine residue might cause the hinge to change the relative positions of the tryptophan residues, thereby opening or closing the channel. In addition, studies on other ion channels have suggested a link between the channel pore and C-terminal regulatory regions via transducing conformational changes to the channel gate^41,42,43. External factors such as phosphatidylinositol 4,5-bisphosphate (PIP₂) can potentially regulate the TRPV5 gating mechanism via binding to the TRP domain in the C-terminus⁴⁴. PIP₂ stabilizes TRPV5 in its open conformation⁴⁵. Interestingly, TRPV5, TRPV6 and the K⁺ channels, which display a similar gating mechanism, are all stimulated by PIP₂^28,46. In contrast, TRPV1 shows no conservation of the tryptophan gate and is inhibited by PIP₂⁴⁴. Our study now demonstrates a link between CaM binding and the channel pore, since the G579A mutation rescues the Ca²⁺ overloading phenotype of the CaM-binding mutants W702A and G698X. Alternatively, loss of CaM binding may transform the inactive G579A mutant into a functional channel through structural rearrangements. Hence, we suggest that C-terminal CaM binding is an essential component of TRPV5 gating by inducing a conformational change of the channel pore and initiating Ca²⁺-dependent channel inactivation. We were unable to test this hypothesis with our current model as the corresponding region was unresolved in the TRPV1 structure. Future TRPV5 models and structures may facilitate viewing this region of the protein and assessing interactions between TM6 and the CaM binding domain.

In conclusion, our study demonstrates a unique mechanism of channel gating for TRPV5 and provides novel insight into TRP channel structure-function relationship, as the tryptophan gate is not conserved in other TRPV family members.

Methods

Buffers

Lysis buffer for cell lysis contained 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% (v/v) Triton X-100, 1 mM sodium orthovanadate, 10 mM Na-β-glycerophosphate, 50 mM Na⁺ fluoride, 10 mM Na⁺ pyrophosphate, 0.27 M sucrose, and the protease inhibitors 1 mM phenylmethanesulfonylfluoride (PMSF), 10 μg/ml leupeptin, 10 μg/ml pepstatin A, 5 μg/ml aprotinin. Buffer A contained 50 mM Tris-HCl (pH 7.5) and 0.1 mM EGTA. TBS-Tween (TBS-T) was Tris-HCl (pH 7.5), 0.15 M NaCl and 0.1% (v/v) Tween-20. Laemmli sample buffer (5X) contained 250 mM Tris-HCl pH 6.8, 10% (w/v) SDS, 30% (v/v) Glycerol, 5% (v/v) β-mercaptoethanol, and 0.02% (w/v) bromophenol blue. KHB buffer for ⁴⁵Ca²⁺ uptake contained 110 mM NaCl, 5 mM KCl, 1.2 mM MgCl₂, 0.1 mM CaCl₂, 10 mM Na-acetate, 2 mM NaH₂PO₄, and 20 mM HEPES (pH 7.4, NaOH). Stop buffer for ⁴⁵Ca²⁺ uptake contained 110 mM NaCl, 5 mM KCl, 1.2 mM MgCl₂, 10 mM Na-acetate, 0.5 mM CaCl₂, 1.5 mM LaCl₃, and 20 mM HEPES (pH 7.4, NaOH).

Generation of a TRPV5 homology model

The YASARA⁴⁷ & WHAT IF⁴⁸ Twinset was used to build a homology model of TRPV5, based on the TRPV1 and TRPV6 structures as a modeling template (29% sequence identity, PDB files 3j5p and 3j5q^18,32, 60% sequence identity, PDB file 5iwk¹⁹). An automatic script with standard parameters was used. The model contained residue 1–644 of rabbit TRPV5. YASARA was used for detailed evaluation of the models.

The generated homology model was further visualized in COOT⁴⁹ and pore radii were analyzed using the HOLE program⁵⁰.

Cell culture

HEK293 cells were purchased from ATCC (LGC Standards GmbH, Wesel, Germany) and grown in Dulbecco’s modified eagle’s medium (DMEM, Bio Whittakker-Europe, Verviers, Belgium) containing 10% (v/v) fetal calf serum (PAA, Liz Australia), 2 mM L-glutamine and 10 μg/ml non-essential amino acids at 37 °C in a humidity-controlled incubator with 5% (v/v) CO₂. HEK293 cells were transiently transfected with the respective DNA construct using polyethyleneimine (PEI, Brunswig/Polysciences Inc) with a DNA:PEI ratio of 1:6 for the Fura-2 imaging and all biochemical experiments. For the patch clamp experiments, plasmids were transiently transfected using lipofectamine 2000 (Invitrogen, Breda, The Netherlands) in a DNA:lipofectamine ratio of 1:2. Cells were used 24–36 hours after transfection.

DNA constructs

The pCINeo/IRES-GFP plasmid encompassing HA-tagged rabbit TRPV5 was generated as described previously^14,51. Restriction enzyme digestions, DNA ligations and other recombinant DNA procedures were performed according to standard protocols. Mutations were introduced using the QuikChange site-directed mutagenesis method (Stratagene, La Jolla, USA), following the manufacturer’s protocol. All constructs were verified by DNA sequencing.

Cell lysis and immunoblotting

Cells were disrupted in ice-cold lysis buffer, and the lysates were clarified by centrifugation at 4°C for 15 minutes at 16,000 g. Protein concentration was measured using the Bradford method, according to standard protocol. Cell lysates in Laemmli sample buffer were subjected to 8% (w/v) SDS-PAGE, transferred to polyvinylidene fluoride (PDVF) membranes. Membranes were blocked for 30 minutes with 5% (w/v) non-fat dry milk (in TBS-T) and immunoblotted overnight at 4 °C using anti-HA antibody (1:5,000, Cell Signalling Technology, Beverly, MA, USA) or anti-beta-Actin (1:10,000, Sigma A5441). Subsequently, the blots were washed with TBS-T, incubated with secondary peroxidase-labelled goat anti-mouse IgG (1:10,000, Chemie Brunschwig, Basel, Switzerland) for 1 hour at room temperature. After subsequent washes, they were visualized with enhanced chemiluminescence reagent using the Bio-Rad ChemiDoc XRS imaging system.

Cell surface biotinylation assay

One day after transfection, HEK293 cells were reseeded on poly-L-lysine (Sigma, St Louis, MO, USA) coated (0.1 mg/ml) 6-well plates. Subsequently, cells were biotinylated as described previously⁵² and disrupted in 0.5 ml lysis buffer. Lysates were clarified by centrifugation at 4 °C for 15 minutes at 16,000 g and the biotinylated proteins were precipitated via incubation with neutravidin beads (Pierce, Ettenleur, the Netherlands) for 2 hours at 4 °C. The precipitates were washed three times with lysis buffer and two times with buffer A. After elution by 2X Laemmli sample buffer, TRPV5 protein expression at the cell surface and in total cell lysate was determined by immunoblotting using anti-HA antibody.

CaM binding assay

The lysis buffer for cell lysis contained either 1 mM Ca²⁺ or 5 mM EGTA (negative control). Following, 1 mg of clarified cell lysate was incubated with CaM agarose beads (Sigma) for 2 hours at 4 °C under gentle agitation. Subsequently, the immunoprecipitates were washed three times with lysis buffer containing 1 mM Ca²⁺ or 5 mM EGTA and then twice with Buffer A. Proteins were eluted by re-suspending the immunoprecipitates in 30 μl of 2X Laemmli sample buffer.

⁴⁵Ca²⁺ uptake assay

One day after transfection, HEK293 cells were reseeded on poly-L-lysine coated (0.1 mg/ml) 24-well plates. Radioactive ⁴⁵Ca²⁺ uptake was performed as previously described⁵². In short, cells were pretreated for 30 min with 25 μM BAPTA-AM at 37 °C, subsequently washed twice with warm KHB buffer, and then incubated for 10 min at 37 °C with ⁴⁵Ca (1 μCi/ml) in KHB buffer containing the voltage-gated Ca²⁺ channel blockers felodipine (10 μM) and verapamil (10 μM). Ruthenium red (10 μM) was used for blocking the TRPV5-mediated ⁴⁵Ca²⁺ uptake. After 10 min, the cells were washed three times with ice-cold stop buffer, and lysed in 0,05% SDS. This was transferred to tubes containing scintillation solution and the amount of ⁴⁵Ca was measured using a scintillation counter.

Intracellular Ca²⁺ measurements using fura-2-AM

Cells were seeded on fibronectin (Roche, Indianapolis, USA) coated coverslips (diameter 25 mm) and transfected with the appropriate TRPV5 constructs. The next day, cells were loaded for 20 minutes with 3 μM fura-2-acetoxymethyl ester (fura-2-AM; Molecular Probes) and 0.01% (v/v) Pluronic F-129 (Molecular Probes) in Krebs solution (5.5 mM KCl, 147 mM NaCl, 1.2 mM MgCl₂, 1.5 mM CaCl₂, 10 mM glucose, and 10 mM HEPES/NaOH, pH 7.4) at 37 °C. Subsequently, the cells were washed once with Krebs solution and allowed to equilibrate at 37 °C for another 10 minutes. Details of microscopy procedures and quantitative image analysis have been described previously¹⁷. In short, fura-2-loaded cells were placed on an inverted microscope using an incubation chamber containing Krebs solution and intracellular Ca²⁺ levels were calculated from the fluorescence emission ratio of 340 and 380 nm excitation. Our data shows basal Ca²⁺ levels that were measured immediately at t = 0, the start of the experiment. All measurements were performed at room temperature.

Electrophysiology

All patch clamp recordings were acquired using an EPC-9 amplifier and the Patchmaster software (HEKA electronics, Lambrecht, Germany). The sampling interval was set to 200 μs for whole-cell recordings and 100 μs for single-channel recordings. A low-pass filter set at 3.6 kHz was used for whole-cell recordings, and single-channel recordings had a low-pass filter set at 5 kHz. The Humbug 50/60 Hz noise eliminator (Quest Scientific, Vancouver, Canada) was used for reduction of electrical noise. Pipettes for whole-cell recordings were pulled from thin-walled borosilicate glass (Harvard Apparatus, March-Hugstetten, Germany) and had resistances between 1 and 3 MΩ. Series resistance compensation was set to 75–95% in all whole-cell experiments. Pipettes for single-channel recordings were pulled from thick-walled borosilicate glass (Harvard Apparatus) and had resistances between 8 and 11 MΩ. The extracellular solutions for whole-cell recordings were: 1) nominally divalent-free (nDVF) solution containing (in mM) 150 NaCl, 6 CsCl, 10 glucose, 10 HEPES/NaOH, pH 7.4; and 2) divalent-free (DVF) solution, which is nDVF solution with addition of 100 μM EGTA. Hence, the EGTA-sensitive whole-cell current was obtained by perfusion of DVF. The pipette solution is composed of (in mM) 20 CsCl, 100 CsAspartate, 1 MgCl₂, 10 BAPTA, 4 Na₂ATP, 10 HEPES/CsOH, pH 7.2. A voltage step protocol, consisting of voltage steps from −100 to +40 mV, was applied from a holding potential of +20 mV. The extracellular solution for cell-attached measurements contained (in mM) 150 NaCl, 6 CsCl, 10 glucose, 10 HEPES/NaOH, pH 7.4. The pipette solution comprised (in mM) 140 NaCl, 10 EGTA, 10 HEPES/NaOH, pH 7.2. Single channel activity was monitored upon a 10 s step to −80 mV from a holding potential 0 mV. All experiments were performed at room temperature. The analysis and display of whole-cell recordings were performed using Igor Pro software (WaveMetrics, Lake Oswego, USA). Single channel recordings and dwell time analysis were analyzed using the Qub software package (www.qub.buffalo.edu). The distribution of the channel open and closed states was fitted to an exponential fit and he time constant of each exponential is related to the rate of kinetic state transitions.

Trypan blue assay

Two days after transfection, cells were resuspended in phosphate buffered saline (PBS), pH 7.4, adding 4% (w/v) trypan blue (Sigma) to a final concentration of 0.08% (w/v). The number of non-viable cells was counted using a hemocytometer. Cell death is measured as the number of non-viable cells divided by the total number of cells within the grids on the hemocytometer.

Ethics statement

Research was carried out in accordance with the relevant guidelines and regulations of the Radboud Institute for Molecular Life Sciences, Radboudumc. Experimental protocols were approved by the Radboud Institute for Molecular Life Sciences, Radboudumc.

Statistical analysis

All data are shown as mean ± standard error of the mean (SEM). Statistical significance (p < 0.05) was determined by a one-way ANOVA and a Dunnett Multiple Comparison post-hoc test. Data were analyzed using Prims 6 software (Graphpad Software Inc, La Jolla, CA, USA).

Additional Information

How to cite this article: van der Wijst, J. et al. A Gate Hinge Controls the Epithelial Calcium Channel TRPV5. Sci. Rep. 7, 45489; doi: 10.1038/srep45489 (2017).

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References

Nilius, B. & Voets, T. TRP channels: a TR(I)P through a world of multifunctional cation channels. Pflugers Arch. 451, 1–10 (2005).
Article CAS PubMed Google Scholar
Ramsey, I. S., Delling, M. & Clapham, D. E. An introduction to TRP channels. Annu. Rev. Physiol. 68, 619–647 (2006).
Article CAS PubMed Google Scholar
Venkatachalam, K. & Montell, C. TRP channels. Annu. Rev. Biochem. 76, 387–417 (2007).
Article CAS PubMed PubMed Central Google Scholar
Stock, L., Souza, C. & Treptow, W. Structural basis for activation of voltage-gated cation channels. Biochemistry 52, 1501–1513 (2013).
Article CAS PubMed Google Scholar
Hoenderop, J. G. J. & Bindels, R. J. M. Calciotropic and magnesiotropic TRP channels. Physiology (Bethesda) 23, 32–40 (2008).
CAS Google Scholar
Hoenderop, J. G. J., Nilius, B. & Bindels, R. J. M. ECaC: the gatekeeper of transepithelial Ca2+ transport. Biochim. Biophys. Acta 1600, 6–11 (2002).
Article CAS PubMed Google Scholar
Boros, S., Bindels, R. J. M. & Hoenderop, J. G. J. Active Ca(2+) reabsorption in the connecting tubule. Pflugers Arch. 458, 99–109 (2009).
Article CAS PubMed Google Scholar
Vennekens, R. et al. Permeation and gating properties of the novel epithelial Ca(2+) channel. J. Biol. Chem. 275, 3963–3969 (2000).
Article CAS PubMed Google Scholar
Hoenderop, J. G. et al. The epithelial calcium channel, ECaC, is activated by hyperpolarization and regulated by cytosolic calcium. Biochem. Biophys. Res. Commun. 261, 488–492 (1999).
Article CAS PubMed Google Scholar
Jean, K. et al. Role of aspartate residues in Ca(2+) affinity and permeation of the distal ECaC1. Am. J. Physiol., Cell Physiol. 282, C665–72 (2002).
Article CAS Google Scholar
Nilius, B. et al. The single pore residue Asp542 determines Ca2+ permeation and Mg2+ block of the epithelial Ca2+ channel. J. Biol. Chem. 276, 1020–1025 (2001).
Article CAS PubMed Google Scholar
Nilius, B. et al. Modulation of the epithelial calcium channel, ECaC, by intracellular Ca2+ . Cell Calcium 29, 417–428 (2001).
Article CAS PubMed Google Scholar
Nilius, B. et al. Fast and slow inactivation kinetics of the Ca2+ channels ECaC1 and ECaC2 (TRPV5 and TRPV6). Role of the intracellular loop located between transmembrane segments 2 and 3. J. Biol. Chem. 277, 30852–30858 (2002).
Article CAS PubMed Google Scholar
Lambers, T. T., Weidema, A. F., Nilius, B., Hoenderop, J. G. J. & Bindels, R. J. M. Regulation of the mouse epithelial Ca2(+) channel TRPV6 by the Ca(2+)-sensor calmodulin. J. Biol. Chem. 279, 28855–28861 (2004).
Article CAS PubMed Google Scholar
de Groot, T. et al. Molecular mechanisms of calmodulin action on TRPV5 and modulation by parathyroid hormone. Mol. Cell. Biol. 31, 2845–2853 (2011).
Article CAS PubMed PubMed Central Google Scholar
Nilius, B. et al. The carboxyl terminus of the epithelial Ca(2+) channel ECaC1 is involved in Ca(2+)-dependent inactivation. Pflugers Arch. 445, 584–588 (2003).
Article CAS PubMed Google Scholar
de Groot, T., Verkaart, S., Xi, Q., Bindels, R. J. M. & Hoenderop, J. G. J. The identification of Histidine 712 as a critical residue for constitutive TRPV5 internalization. J. Biol. Chem. 285, 28481–28487 (2010).
Article CAS PubMed PubMed Central Google Scholar
Liao, M., Cao, E., Julius, D. & Cheng, Y. Structure of the TRPV1 ion channel determined by electron cryo-microscopy. Nature 504, 107–112 (2013).
Article ADS CAS PubMed PubMed Central Google Scholar
Saotome, K., Singh, A. K., Yelshanskaya, M. V. & Sobolevsky, A. I. Crystal structure of the epithelial calcium channel TRPV6. Nature 534, 506–511 (2016).
Article ADS CAS PubMed PubMed Central Google Scholar
Zubcevic, L. et al. Cryo-electron microscopy structure of the TRPV2 ion channel. Nat. Struct. Mol. Biol., doi: 10.1038/nsmb.3159 (2016).
Huynh, K. W. et al. Structure of the full-length TRPV2 channel by cryo-EM. Nat Commun 7, 11130 (2016).
Article ADS CAS PubMed PubMed Central Google Scholar
Paulsen, C. E., Armache, J.-P., Gao, Y., Cheng, Y. & Julius, D. Structure of the TRPA1 ion channel suggests regulatory mechanisms. Nature 520, 511–517 (2015).
Article ADS CAS PubMed PubMed Central Google Scholar
Susankova, K., Ettrich, R., Vyklicky, L., Teisinger, J. & Vlachova, V. Contribution of the putative inner-pore region to the gating of the transient receptor potential vanilloid subtype 1 channel (TRPV1). J. Neurosci. 27, 7578–7585 (2007).
Article CAS PubMed PubMed Central Google Scholar
Nilius, B. et al. Pharmacological modulation of monovalent cation currents through the epithelial Ca2+ channel ECaC1. Br. J. Pharmacol. 134, 453–462 (2001).
Article CAS PubMed PubMed Central Google Scholar
Sander, C. & Schneider, R. Database of homology-derived protein structures and the structural meaning of sequence alignment. Proteins 9, 56–68 (1991).
Article CAS PubMed Google Scholar
Dodier, Y., Dionne, F., Raybaud, A., Sauvé, R. & Parent, L. Topology of the selectivity filter of a TRPV channel: rapid accessibility of contiguous residues from the external medium. Am. J. Physiol., Cell Physiol. 293, C1962–70 (2007).
Article CAS Google Scholar
Chinea, G., Padron, G., Hooft, R. W., Sander, C. & Vriend, G. The use of position-specific rotamers in model building by homology. Proteins 23, 415–421 (1995).
Article CAS PubMed Google Scholar
Zhou, H.-X. & McCammon, J. A. The gates of ion channels and enzymes. Trends Biochem. Sci. 35, 179–185 (2010).
Article CAS PubMed Google Scholar
Ma, C. et al. Asp44 Stabilizes the Trp41 Gate of the M2 Proton Channel of Influenza A Virus. Structure 21, 2033–2041 (2013).
Article CAS PubMed PubMed Central Google Scholar
Pilpel, Y., Ben-Tal, N. & Lancet, D. kPROT: a knowledge-based scale for the propensity of residue orientation in transmembrane segments. Application to membrane protein structure prediction. J. Mol. Biol. 294, 921–935 (1999).
CAS PubMed Google Scholar
Maguire, M. E. & Cowan, J. A. Magnesium chemistry and biochemistry. Biometals 15, 203–210 (2002).
Article CAS PubMed Google Scholar
Cao, E., Liao, M., Cheng, Y. & Julius, D. TRPV1 structures in distinct conformations reveal activation mechanisms. Nature 504, 113–118 (2013).
Article ADS CAS PubMed PubMed Central Google Scholar
Tang, Y., Zaitseva, F., Lamb, R. A. & Pinto, L. H. The gate of the influenza virus M2 proton channel is formed by a single tryptophan residue. J. Biol. Chem. 277, 39880–39886 (2002).
Article CAS PubMed Google Scholar
Cuello, L. G. et al. Structural basis for the coupling between activation and inactivation gates in K+ channels. Nature 466, 272–275 (2010).
Article ADS CAS PubMed PubMed Central Google Scholar
Elinder, F., Nilsson, J. & Århem, P. On the opening of voltage-gated ion channels. Physiology & Behavior 92, 1–7 (2007).
Article CAS Google Scholar
Kuo, A. et al. Crystal structure of the potassium channel KirBac1.1 in the closed state. Science 300, 1922–1926 (2003).
Article ADS CAS PubMed Google Scholar
Vemparala, S., Domene, C. & Klein, M. L. Interaction of Anesthetics with Open and Closed Conformations of a Potassium Channel Studied via Molecular Dynamics and Normal Mode Analysis. Biophys. J. 94, 4260–4269 (2008).
Article ADS CAS PubMed PubMed Central Google Scholar
Kuo, A., Domene, C., Johnson, L. N., Doyle, D. A. & Vénien-Bryan, C. Two Different Conformational States of the KirBac3.1 Potassium Channel Revealed by Electron Crystallography. Structure 13, 1463–1472 (2005).
Article CAS PubMed Google Scholar
Yeh, B. I., Sun, T.-J., Lee, J. Z., Chen, H.-H. & Huang, C.-L. Mechanism and molecular determinant for regulation of rabbit transient receptor potential type 5 (TRPV5) channel by extracellular pH. J. Biol. Chem. 278, 51044–51052 (2003).
Article CAS PubMed Google Scholar
Yeh, B. I., Kim, Y. K., Jabbar, W. & Huang, C.-L. Conformational changes of pore helix coupled to gating of TRPV5 by protons. EMBO J. 24, 3224–3234 (2005).
Article CAS PubMed PubMed Central Google Scholar
Schumacher, M. A., Rivard, A. F., Bächinger, H. P. & Adelman, J. P. Structure of the gating domain of a Ca2+-activated K+ channel complexed with Ca2+/calmodulin. Nature 410, 1120–1124 (2001).
Article ADS CAS PubMed Google Scholar
Paoletti, P., Young, E. C. & Siegelbaum, S. A. C-Linker of cyclic nucleotide-gated channels controls coupling of ligand binding to channel gating. J. Gen. Physiol. 113, 17–34 (1999).
Article CAS PubMed PubMed Central Google Scholar
García-Sanz, N. et al. Identification of a tetramerization domain in the C terminus of the vanilloid receptor. J. Neurosci. 24, 5307–5314 (2004).
Article PubMed PubMed Central CAS Google Scholar
Rohács, T., Lopes, C. M. B., Michailidis, I. & Logothetis, D. E. PI(4,5)P2 regulates the activation and desensitization of TRPM8 channels through the TRP domain. Nat. Neurosci. 8, 626–634 (2005).
Article PubMed CAS Google Scholar
Lee, J., Cha, S.-K., Sun, T.-J. & Huang, C.-L. PIP2 activates TRPV5 and releases its inhibition by intracellular Mg2+. J. Gen. Physiol. 126, 439–451 (2005).
Article CAS PubMed PubMed Central Google Scholar
Suh, B.-C. & Hille, B. Regulation of ion channels by phosphatidylinositol 4,5-bisphosphate. Current Opinion in Neurobiology 15, 370–378 (2005).
Article CAS PubMed Google Scholar
Krieger, E., Koraimann, G. & Vriend, G. Increasing the precision of comparative models with YASARA NOVA–a self-parameterizing force field. Proteins 47, 393–402 (2002).
Article CAS PubMed Google Scholar
Vriend, G. WHAT IF: a molecular modeling and drug design program. J Mol Graph 8, 52–6–29 (1990).
Article CAS PubMed Google Scholar
Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. Features and development of Coot. Acta Crystallogr. D Biol. Crystallogr. 66, 486–501 (2010).
Article CAS PubMed PubMed Central Google Scholar
Smart, O. S., Neduvelil, J. G., Wang, X., Wallace, B. A. & Sansom, M. S. HOLE: a program for the analysis of the pore dimensions of ion channel structural models. J Mol Graph 14, 354–60–376 (1996).
van de Graaf, S. F. J. et al. Functional expression of the epithelial Ca(2+) channels (TRPV5 and TRPV6) requires association of the S100A10-annexin 2 complex. EMBO J. 22, 1478–1487 (2003).
Article CAS PubMed PubMed Central Google Scholar
de Groot, T. et al. Parathyroid hormone activates TRPV5 via PKA-dependent phosphorylation. Journal of the American Society of Nephrology 20, 1693–1704 (2009).
Article CAS PubMed PubMed Central Google Scholar

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Acknowledgements

The authors thank Drs. Gert Vriend and Yifan Cheng for helpful suggestions and discussions. This study was financially supported by the Netherlands Organization for Scientific Research (VENI 863.13.010 and VICI 016.130.668) and the European Union’s Seventh Framework Programme (FP7/2007–2013, agreement n° 305608).

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Authors and Affiliations

Department of Physiology, Radboud Institute for Molecular Life Sciences, Radboud university medical center, Nijmegen, The Netherlands,
Jenny van der Wijst, Elizabeth H. Leunissen, Maxime G. Blanchard, Sjoerd Verkaart, René J. Bindels & Joost G. Hoenderop
Centre for Molecular and Biomolecular Informatics, Radboud Institute for Molecular Life Sciences, Radboud university medical center, Nijmegen, The Netherlands
Hanka Venselaar
Department of Physiology, University of California, San Francisco, California, 94158-2517, USA
Candice E. Paulsen

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Jenny van der Wijst
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Elizabeth H. Leunissen
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Contributions

J.W., E.H.L., M.G.B., S.V., R.J.B. and J.G.H. designed, carried out and analyzed the experiments. H.V. and C.E.P. prepared and analyzed the homology model. J.W., R.J.B., and J.G.H wrote the manuscript with input from all authors. Funding acquisition is done by J.W., R.J.B. and J.G.H.

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Correspondence to Joost G. Hoenderop.

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van der Wijst, J., Leunissen, E., Blanchard, M. et al. A Gate Hinge Controls the Epithelial Calcium Channel TRPV5. Sci Rep 7, 45489 (2017). https://doi.org/10.1038/srep45489

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Received: 19 October 2016
Accepted: 27 February 2017
Published: 04 April 2017
DOI: https://doi.org/10.1038/srep45489

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