Showing 1–21 of 21 results

  1. Reviews |

    • Proteinprotein interactions (PPIs) are of pivotal importance in the regulation of biological systems and are consequently... implicated in the development of disease states. Recent work... ...begun to show that, with the right tools, certain classes of PPI can yield to the efforts of medicinal...the
  2. Research | | Open

    • Natural selection shapes protein solubility to physiological requirements and recombinant... applications that require higher protein concentrations are often problematic.... This raises the question whether the solubility of natural protein sequences can be improved.... ...correlation between the...
  3. Research |

    • The potential utility of synthetic macrocycles (MCs) as... ...low-druggability targets such as protein-protein interactions, has been widely... ...information, however, to guide the design of MCs for good target protein–binding activity or bioavailability.... ...knowledge gap, we analyze the binding...
  4. Research | | Open

    • ...typically yield several candidates capable of engaging a given target. The development steps that follow,... ...be resource demanding, thereby increasing the risk of failure of the overall antibody discovery program.... In particular, insufficient solubility, which may lead to... ...hinders the...
  5. Research | | Open

  6. Reviews |

    • Historically, targeting proteinprotein interactions with small molecules was... not thought possible because the corresponding interfaces were considered mostly... ...peptides and antibodies. However, the past decade has seen encouraging... breakthroughs through the refinement of existing...
    • ...article begins with a discussion of fundamental differences between substrates and... inhibitors, and some of the assumptions and goals underlying the design of a new ligand to a... target protein. An overview is given of the methods currently used to locate... ...sites on protein surfaces,...
  7. | Open

    • Protein nanocages have been explored as... ...carriers in biomedicine. Formed by the self-assembly of protein subunits, the caged structure has three surfaces... that can be engineered: the interior, the exterior and the intersubunit. Therapeutic and diagnostic... ...have been loaded in the... ...of...
  8. Reviews |

    • ...nuclear lamins and those in the cytoplasm (keratins, vimentin,... ...neurofilaments and glial fibrillary acidic protein, among others), are... ...(PTMs). Proteomic advances highlight the enormous complexity and regulatory potential... of IF protein PTMs, which include phosphorylation,... ...for...
  9. Research |

    • ...lipids—a palmitoyl group at the N terminus and a cholesteroyl... group at the C terminus. While the palmitoyl group binds and inactivates... Patched 1 (PTCH1), the main receptor for HH ligands, the function of the cholesterol modification has remained mysterious... ...structural and biochemical...
  10. Research |

    • Expression of hypoxia-responsive genes is mediated... by the heterodimeric transcription factor hypoxia-inducible... ...HIF-1) in complex with the p300/CREB-binding protein (p300/CBP) transcriptional coactivator... . The protein CITED2, which binds p300/... ...negative regulator of HIF-1... ...the...
  11. Research | | Open

    • ...specific dsDNA sequences in vitro, the N-terminal WH1 domain of the plasmid DNA replication initiator RepA... ...amyloid fibres. These are bundles of single or double twisted tubular... ...distorted RepA-WH1 monomers are the building blocks. When expressed... ...-WH1 triggers the first... ...of...the
  12. Research |

    • ...on Earth. Two large membrane protein complexes, photosystem I and... ...act in series to catalyse the light-driven reactions in photosynthesis... . PSII catalyses the light-driven water splitting process... , which maintains the Earth’s oxygenic atmosphere1.... ..., the oxygen-evolving complex...
  13. Research | | Open

    • Mamba venoms contain a multiplicity of three-finger fold aminergic toxins... ...generate novel functions on this structural scaffold and to avoid the daunting task of producing and screening an overwhelming... number of variants generated by a classical... protein engineering strategy, we accepted
  14. Research |

    • ...(p53-induced RING-H2 domain protein; also known as Rchy1)... ...is a unique cysteine-rich protein comprising three modular domains. The protein binds nine zinc ions using... a variety of zinc coordination schemes, including... ...p53 interaction is dependent on the C-terminal zinc binding...
  15. Research |

    • BCL-2-associated X protein (BAX) is a critical... ...screens on this aggregation-prone protein. Here, we overcame prior... ...an NMR-based fragment screen of full-length human BAX.... ...to a pocket formed by the junction of the α3–α4 and α... ...6 hairpins. Biochemical and structural analyses...
  16. Reviews |

    • The heparan sulphate proteoglycan agrin is... ...known as a crucial organizer of postsynaptic differentiation at the neuromuscular junction, but it... ...recently also been implicated in the formation of the immunological synapse, the organization of the cytoskeleton and the amelioration of...
  17. Reviews |

    • The increasing use of recombinantly expressed therapeutic proteins in the pharmaceutical industry has highlighted issues... ...long-term storage and means of efficacious delivery that avoid adverse... ...fulfilled some but not all of their promises, while hydrogels... ...Strategies to curb the...
    • 183 feet long for saddle horses and hikers is... the unique feature that made practicable... ...a new trail to shorten the distance from Many Glacier Hotel... ...Lake in Glacier National Park. The improvement project provides an easier... and more interesting route for the tourist and shortens the...
    Scientific American 146 , 358–374
  18. Research |

    • AGONISTS that stimulate protein kinase C (PKC) induce... ...in cell morphology correlating with the reorganization of submembranous actin1,2, but... ...actin assembly has been identified3. The myristoylated, alanine-rich C... ...a predominant, specific substrate of PKC which is phosphorylated...protein
  19. Research |

    • Mediaeval Norse writings that describe the merman are considered accurate observations... of a natural phenomenon. Images of common sea mammals, severely... ...non-uniform atmospheric refraction, fit the mediaeval descriptions remarkably well.... ...: computer-generated images of a killer whale...