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Reverse transcription converts mRNA into cDNA.


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Reverse transcription converts mRNA into cDNA.
cDNA is made from mRNA.

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Through laboratory techniques that track quantities of mRNA, scientists can identify which genes are “turned on” and which are “turned off” in an organism’s tissue at a given time, as well as how vigorously these genes are expressed. This enables scientists to understand the time course of gene expression and the impact of environmental conditions on multiple genes. In this page, you will learn about two techniques that track RNA, Northern blot and SAGE.

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